Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1

Research output: Contribution to journalJournal articlepeer-review

Standard

Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1. / Kang, Sona; Bajnok, Laszlo; Longo, Kenneth A.; Petersen, Rasmus K.; Hansen, Jacob B.; Kristiansen, Karsten; MacDougald, Ormond A.

In: Molecular and Cellular Biology, Vol. 25, No. 4, 2005, p. 1272-1282.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kang, S, Bajnok, L, Longo, KA, Petersen, RK, Hansen, JB, Kristiansen, K & MacDougald, OA 2005, 'Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1', Molecular and Cellular Biology, vol. 25, no. 4, pp. 1272-1282. https://doi.org/10.1128/MCB.25.4.1272-1282.2005

APA

Kang, S., Bajnok, L., Longo, K. A., Petersen, R. K., Hansen, J. B., Kristiansen, K., & MacDougald, O. A. (2005). Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1. Molecular and Cellular Biology, 25(4), 1272-1282. https://doi.org/10.1128/MCB.25.4.1272-1282.2005

Vancouver

Kang S, Bajnok L, Longo KA, Petersen RK, Hansen JB, Kristiansen K et al. Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1. Molecular and Cellular Biology. 2005;25(4):1272-1282. https://doi.org/10.1128/MCB.25.4.1272-1282.2005

Author

Kang, Sona ; Bajnok, Laszlo ; Longo, Kenneth A. ; Petersen, Rasmus K. ; Hansen, Jacob B. ; Kristiansen, Karsten ; MacDougald, Ormond A. / Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 4. pp. 1272-1282.

Bibtex

@article{aef8e1d0a8ef11debc73000ea68e967b,
title = "Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1",
abstract = "Activation of canonical Wnt signaling inhibits brown adipogenesis of cultured cells by impeding induction of PPAR and C/EBP. Although enforced expression of these adipogenic transcription factors restores lipid accumulation and expression of FABP4 in Wnt-expressing cells, additional expression of PGC-1 is required for activation of uncoupling protein 1 (UCP1). Wnt10b blocks brown adipose tissue development and expression of UCP1 when expressed from the fatty acid binding protein 4 promoter, even when mice are administered a {\ss}3-agonist. In differentiated brown adipocytes, activation of Wnt signaling suppresses expression of UCP1 through repression of PGC-1. Consistent with these in vitro observations, UCP1-Wnt10b transgenic mice, which express Wnt10b in interscapular tissue, lack functional brown adipose tissue. While interscapular tissue of UCP1-Wnt10b mice lacks expression of PGC-1 and UCP1, the presence of unilocular lipid droplets and expression of white adipocyte genes suggest conversion of brown adipose tissue to white. Reciprocal expression of Wnt10b with UCP1 and PGC-1 in interscapular tissue from cold-challenged or genetically obese mice provides further evidence for regulation of brown adipocyte metabolism by Wnt signaling. Taken together, these data suggest that activation of canonical Wnt signaling early in differentiation blocks brown adipogenesis, whereas activating Wnt signaling in mature brown adipocytes stimulates their conversion to white adipocytes. ",
author = "Sona Kang and Laszlo Bajnok and Longo, {Kenneth A.} and Petersen, {Rasmus K.} and Hansen, {Jacob B.} and Karsten Kristiansen and MacDougald, {Ormond A.}",
year = "2005",
doi = "10.1128/MCB.25.4.1272-1282.2005",
language = "English",
volume = "25",
pages = "1272--1282",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "4",

}

RIS

TY - JOUR

T1 - Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1

AU - Kang, Sona

AU - Bajnok, Laszlo

AU - Longo, Kenneth A.

AU - Petersen, Rasmus K.

AU - Hansen, Jacob B.

AU - Kristiansen, Karsten

AU - MacDougald, Ormond A.

PY - 2005

Y1 - 2005

N2 - Activation of canonical Wnt signaling inhibits brown adipogenesis of cultured cells by impeding induction of PPAR and C/EBP. Although enforced expression of these adipogenic transcription factors restores lipid accumulation and expression of FABP4 in Wnt-expressing cells, additional expression of PGC-1 is required for activation of uncoupling protein 1 (UCP1). Wnt10b blocks brown adipose tissue development and expression of UCP1 when expressed from the fatty acid binding protein 4 promoter, even when mice are administered a ß3-agonist. In differentiated brown adipocytes, activation of Wnt signaling suppresses expression of UCP1 through repression of PGC-1. Consistent with these in vitro observations, UCP1-Wnt10b transgenic mice, which express Wnt10b in interscapular tissue, lack functional brown adipose tissue. While interscapular tissue of UCP1-Wnt10b mice lacks expression of PGC-1 and UCP1, the presence of unilocular lipid droplets and expression of white adipocyte genes suggest conversion of brown adipose tissue to white. Reciprocal expression of Wnt10b with UCP1 and PGC-1 in interscapular tissue from cold-challenged or genetically obese mice provides further evidence for regulation of brown adipocyte metabolism by Wnt signaling. Taken together, these data suggest that activation of canonical Wnt signaling early in differentiation blocks brown adipogenesis, whereas activating Wnt signaling in mature brown adipocytes stimulates their conversion to white adipocytes.

AB - Activation of canonical Wnt signaling inhibits brown adipogenesis of cultured cells by impeding induction of PPAR and C/EBP. Although enforced expression of these adipogenic transcription factors restores lipid accumulation and expression of FABP4 in Wnt-expressing cells, additional expression of PGC-1 is required for activation of uncoupling protein 1 (UCP1). Wnt10b blocks brown adipose tissue development and expression of UCP1 when expressed from the fatty acid binding protein 4 promoter, even when mice are administered a ß3-agonist. In differentiated brown adipocytes, activation of Wnt signaling suppresses expression of UCP1 through repression of PGC-1. Consistent with these in vitro observations, UCP1-Wnt10b transgenic mice, which express Wnt10b in interscapular tissue, lack functional brown adipose tissue. While interscapular tissue of UCP1-Wnt10b mice lacks expression of PGC-1 and UCP1, the presence of unilocular lipid droplets and expression of white adipocyte genes suggest conversion of brown adipose tissue to white. Reciprocal expression of Wnt10b with UCP1 and PGC-1 in interscapular tissue from cold-challenged or genetically obese mice provides further evidence for regulation of brown adipocyte metabolism by Wnt signaling. Taken together, these data suggest that activation of canonical Wnt signaling early in differentiation blocks brown adipogenesis, whereas activating Wnt signaling in mature brown adipocytes stimulates their conversion to white adipocytes.

U2 - 10.1128/MCB.25.4.1272-1282.2005

DO - 10.1128/MCB.25.4.1272-1282.2005

M3 - Journal article

C2 - 15684380

VL - 25

SP - 1272

EP - 1282

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 4

ER -

ID: 14667044