Genomic organization of the mouse peroxisome proliferator-activatedreceptor b/d gene: alternative promoter usage and splicing yield transcriptsexhibiting differential translational efficiency
Research output: Contribution to journal › Journal article › peer-review
Standard
Genomic organization of the mouse peroxisome proliferator-activatedreceptor b/d gene: alternative promoter usage and splicing yield transcriptsexhibiting differential translational efficiency. / Larsen, L.K.; Amri, E.-Z.; Mandrup, S.; Pacot, C.; Kristiansen, K.
In: Biochemical Journal, Vol. 366, 2002, p. 767-75.Research output: Contribution to journal › Journal article › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Genomic organization of the mouse peroxisome proliferator-activatedreceptor b/d gene: alternative promoter usage and splicing yield transcriptsexhibiting differential translational efficiency
AU - Larsen, L.K.
AU - Amri, E.-Z.
AU - Mandrup, S.
AU - Pacot, C.
AU - Kristiansen, K.
N1 - Key words: expression, nuclear hormone receptor, translation.
PY - 2002
Y1 - 2002
N2 - Peroxisome proliferator-activated receptor (PPAR) b}d is ubiquitouslyexpressed, but the level of expression differs markedlybetween different cell types. In order to determine the molecularmechanisms governing PPARb}d gene expression, wehave isolated and characterized the mouse gene encodingPPARb}d. The gene spans approx. 41 kb and comprises 11 exonsof which the six exons located in the 3«-end of the gene areincluded in all transcripts. Primer-extension and 5«-rapid ampli-®cation of cDNA ends experiments revealed the presence ofmultiple transcription start points and splice variants, originatingfrom the use of at least four different promoters. One of these transcription start points was found to be used predominantly inall tissues examined. Initiation from this major transcriptionstart point gives rise to a transcript with a 548 nt 5«-untranslatedleader containing eight upstream AUG codons. We show thatthe presence of the 548 nt leader resulted in a low translationalefficiency of the corresponding PPARb}d mRNA and propose,based on structural features of the 5«-untranslated region, thattranslational initiation may be mediated via an internal ribosomeentry site-dependent mechanism.
AB - Peroxisome proliferator-activated receptor (PPAR) b}d is ubiquitouslyexpressed, but the level of expression differs markedlybetween different cell types. In order to determine the molecularmechanisms governing PPARb}d gene expression, wehave isolated and characterized the mouse gene encodingPPARb}d. The gene spans approx. 41 kb and comprises 11 exonsof which the six exons located in the 3«-end of the gene areincluded in all transcripts. Primer-extension and 5«-rapid ampli-®cation of cDNA ends experiments revealed the presence ofmultiple transcription start points and splice variants, originatingfrom the use of at least four different promoters. One of these transcription start points was found to be used predominantly inall tissues examined. Initiation from this major transcriptionstart point gives rise to a transcript with a 548 nt 5«-untranslatedleader containing eight upstream AUG codons. We show thatthe presence of the 548 nt leader resulted in a low translationalefficiency of the corresponding PPARb}d mRNA and propose,based on structural features of the 5«-untranslated region, thattranslational initiation may be mediated via an internal ribosomeentry site-dependent mechanism.
M3 - Journal article
VL - 366
SP - 767
EP - 775
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
ER -
ID: 10242320