Induction of acyl-CoA-binding protein and its mRNA in 3T3-L1 cells by insulin during preadipocyte-to-adipocyte differentiation
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The induction of acyl-CoA-binding protein (ACBP) and ACBP mRNA was investigated in 3T3-L1 cells during growth and insulin-induced differentiation. The level of ACBP relative to both total soluble protein and DNA increased during insulin-stimulated conversion of 3T3-L1 cells from preadipocytes into fully developed adipocytes. So did the total rate of lipogenesis, as measured by incorporation of [1-14C]acetate. A similar increase in ACBP mRNA relative to total RNA was observed. These results therefore suggest that ACBP plays a specific role in the lipogenic process. However, this role might be indirect, as the increase in lipogenesis preceded the increase in ACBP. The significance of this finding is discussed.
Original language | English |
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Journal | Biochemical Journal |
Volume | 277 ( Pt 2) |
Pages (from-to) | 341-4 |
Number of pages | 3 |
ISSN | 0264-6021 |
Publication status | Published - 1991 |
Bibliographical note
Keywords: Acetates; Adipose Tissue; Animals; Carrier Proteins; Cell Differentiation; Cell Line; Cloning, Molecular; Dexamethasone; Diazepam Binding Inhibitor; Gene Library; Kinetics; Lipids; Liver; Mice; RNA, Messenger; Rats; Rats, Inbred Strains; Time Factors
ID: 11255484