Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa
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Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa. / Kirpekar, F; Nordhoff, E; Kristiansen, K; Roepstorff, P; Lezius, A; Hahner, S; Karas, M; Hillenkamp, F.
In: Nucleic Acids Research, Vol. 22, No. 19, 1994, p. 3866-70.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa
AU - Kirpekar, F
AU - Nordhoff, E
AU - Kristiansen, K
AU - Roepstorff, P
AU - Lezius, A
AU - Hahner, S
AU - Karas, M
AU - Hillenkamp, F
N1 - Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA
PY - 1994
Y1 - 1994
N2 - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.
AB - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.
M3 - Journal article
C2 - 7524029
VL - 22
SP - 3866
EP - 3870
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 19
ER -
ID: 11231720