Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

Research output: Contribution to journalJournal articlepeer-review

Standard

Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa. / Kirpekar, F; Nordhoff, E; Kristiansen, K; Roepstorff, P; Lezius, A; Hahner, S; Karas, M; Hillenkamp, F.

In: Nucleic Acids Research, Vol. 22, No. 19, 1994, p. 3866-70.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kirpekar, F, Nordhoff, E, Kristiansen, K, Roepstorff, P, Lezius, A, Hahner, S, Karas, M & Hillenkamp, F 1994, 'Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa', Nucleic Acids Research, vol. 22, no. 19, pp. 3866-70. <http://nar.oxfordjournals.org/cgi/content/abstract/22/19/3866>

APA

Kirpekar, F., Nordhoff, E., Kristiansen, K., Roepstorff, P., Lezius, A., Hahner, S., Karas, M., & Hillenkamp, F. (1994). Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa. Nucleic Acids Research, 22(19), 3866-70. http://nar.oxfordjournals.org/cgi/content/abstract/22/19/3866

Vancouver

Kirpekar F, Nordhoff E, Kristiansen K, Roepstorff P, Lezius A, Hahner S et al. Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa. Nucleic Acids Research. 1994;22(19):3866-70.

Author

Kirpekar, F ; Nordhoff, E ; Kristiansen, K ; Roepstorff, P ; Lezius, A ; Hahner, S ; Karas, M ; Hillenkamp, F. / Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 19. pp. 3866-70.

Bibtex

@article{f2d055100f0b11de8478000ea68e967b,
title = "Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa",
abstract = "Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.",
author = "F Kirpekar and E Nordhoff and K Kristiansen and P Roepstorff and A Lezius and S Hahner and M Karas and F Hillenkamp",
note = "Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA",
year = "1994",
language = "English",
volume = "22",
pages = "3866--70",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "19",

}

RIS

TY - JOUR

T1 - Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

AU - Kirpekar, F

AU - Nordhoff, E

AU - Kristiansen, K

AU - Roepstorff, P

AU - Lezius, A

AU - Hahner, S

AU - Karas, M

AU - Hillenkamp, F

N1 - Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA

PY - 1994

Y1 - 1994

N2 - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

AB - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

M3 - Journal article

C2 - 7524029

VL - 22

SP - 3866

EP - 3870

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 19

ER -

ID: 11231720