Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice
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Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice. / Rebelo, Kemilla Sarmento; Nunez, Carla Evelyn Coimbra; Cazarin, Cinthia Baú Betim; Maróstica Júnior, Mário Roberto; Kristiansen, Karsten; Danneskiold-Samsøe, Niels Banhos.
In: Food & Function, Vol. 13, No. 7, 2022, p. 3982-3992.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Pot-pollen supplementation reduces fasting glucose and modulates the gut microbiota in high-fat/high-sucrose fed C57BL/6 mice
AU - Rebelo, Kemilla Sarmento
AU - Nunez, Carla Evelyn Coimbra
AU - Cazarin, Cinthia Baú Betim
AU - Maróstica Júnior, Mário Roberto
AU - Kristiansen, Karsten
AU - Danneskiold-Samsøe, Niels Banhos
PY - 2022
Y1 - 2022
N2 - Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots. As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity. Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks. In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity. Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia. Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose. Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.
AB - Pot-pollen is a mixture of pollen and nectar from flowers combined with salivary substances of stingless bees, which together are fermented inside cerumen pots. As pot-pollen is rich in polyphenols, we hypothesized that dietary ingestion could modulate obesity, glucose metabolism, and the gut microbiota in an animal model of diet-induced obesity. Male C57BL/6J mice were fed a low-fat/low-sucrose diet (LF/LS), a HF/HS diet or a HF/HS diet containing 0.1% pot-pollen (HF/HS-PP) for 12 weeks. In HF/HS-fed mice, pot-pollen supplementation decreased fasting blood glucose and increased glucose-stimulated insulin secretion without modifying weight gain, body composition, glucose tolerance, and insulin sensitivity. Intake of pot-pollen resulted in changes of the gut microbiota, including a decrease in the abundance of the Rikenellaceae RC9 gut group and Lactobacillus, and an increase in the abundance of Romboutsia. Correlations between genus abundances and metabolic changes in response to supplementation indicated that the gut microbiota contributed to the positive effects of pot-pollen ingestion on fasting glucose. Pot-pollen supplementation-associated changes in the gut microbiota composition correlated with the lowering of fasting glucose levels without modulating weight gain.
KW - Animals
KW - Diet, High-Fat/adverse effects
KW - Dietary Supplements
KW - Fasting
KW - Gastrointestinal Microbiome
KW - Glucose
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Pollen
KW - Sucrose/pharmacology
U2 - 10.1039/d1fo03019a
DO - 10.1039/d1fo03019a
M3 - Journal article
C2 - 35311861
VL - 13
SP - 3982
EP - 3992
JO - Food & Function
JF - Food & Function
SN - 2042-6496
IS - 7
ER -
ID: 309119738