Regulation of ribosome synthesis in Tetrahymena pyriformis. 2. Coordination of synthesis of ribosomal proteins and ribosomal RNA during nutritional shift-up
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Regulation of ribosome synthesis in Tetrahymena pyriformis. 2. Coordination of synthesis of ribosomal proteins and ribosomal RNA during nutritional shift-up. / Dreisig, H; Andreasen, P H; Kristiansen, K.
In: European Journal of Biochemistry, Vol. 140, No. 3, 1984, p. 477-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Regulation of ribosome synthesis in Tetrahymena pyriformis. 2. Coordination of synthesis of ribosomal proteins and ribosomal RNA during nutritional shift-up
AU - Dreisig, H
AU - Andreasen, P H
AU - Kristiansen, K
N1 - Keywords: Animals; Culture Media; Dactinomycin; Kinetics; Polyribosomes; RNA, Ribosomal; Ribosomal Proteins; Ribosomes; Tetrahymena pyriformis
PY - 1984
Y1 - 1984
N2 - The addition of nutrients to long-time-starved cells of Tetrahymena pyriformis leads to a 50-60-fold increase in the rate of synthesis of ribosomal proteins (r-proteins). This is achieved by a 6-fold increase in the relative rate of r-protein synthesis and a 8-10-fold increase in the rate of total protein synthesis. Synthesis of r-proteins constitutes one third of total cellular protein synthesis 2-4 h after refeeding and the absolute rate of r-protein synthesis is approximately three-times greater than in exponentially growing cells. The synthesis of the individual r-proteins is coordinately regulated during a nutritional shift-up, and de novo synthesized r-proteins are stable. Addition of actinomycin D prevents the increase in the rate of r-protein synthesis. The rates of synthesis of rRNA and r-protein increase in concert, implying coordinate regulation. Furthermore, a comparison of the observed accumulation of r-proteins with the predicted accumulation based on the accumulation of rRNA suggests that rRNA and r-protein are synthesized in a stoichiometrically balanced way during the entire refeeding period.
AB - The addition of nutrients to long-time-starved cells of Tetrahymena pyriformis leads to a 50-60-fold increase in the rate of synthesis of ribosomal proteins (r-proteins). This is achieved by a 6-fold increase in the relative rate of r-protein synthesis and a 8-10-fold increase in the rate of total protein synthesis. Synthesis of r-proteins constitutes one third of total cellular protein synthesis 2-4 h after refeeding and the absolute rate of r-protein synthesis is approximately three-times greater than in exponentially growing cells. The synthesis of the individual r-proteins is coordinately regulated during a nutritional shift-up, and de novo synthesized r-proteins are stable. Addition of actinomycin D prevents the increase in the rate of r-protein synthesis. The rates of synthesis of rRNA and r-protein increase in concert, implying coordinate regulation. Furthermore, a comparison of the observed accumulation of r-proteins with the predicted accumulation based on the accumulation of rRNA suggests that rRNA and r-protein are synthesized in a stoichiometrically balanced way during the entire refeeding period.
U2 - 10.1111/j.1432-1033.1984.tb08127.x
DO - 10.1111/j.1432-1033.1984.tb08127.x
M3 - Journal article
C2 - 6426954
VL - 140
SP - 477
EP - 483
JO - FEBS Journal
JF - FEBS Journal
SN - 1742-464X
IS - 3
ER -
ID: 11368491