Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein

Research output: Contribution to journalJournal articlepeer-review

Standard

Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein. / Elholm, M; Bjerking, G; Knudsen, J; Kristiansen, K; Mandrup, S.

In: Gene, Vol. 173, No. 2, 1996, p. 233-8.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Elholm, M, Bjerking, G, Knudsen, J, Kristiansen, K & Mandrup, S 1996, 'Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein', Gene, vol. 173, no. 2, pp. 233-8. https://doi.org/10.1016/0378-1119(96)00213-2

APA

Elholm, M., Bjerking, G., Knudsen, J., Kristiansen, K., & Mandrup, S. (1996). Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein. Gene, 173(2), 233-8. https://doi.org/10.1016/0378-1119(96)00213-2

Vancouver

Elholm M, Bjerking G, Knudsen J, Kristiansen K, Mandrup S. Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein. Gene. 1996;173(2):233-8. https://doi.org/10.1016/0378-1119(96)00213-2

Author

Elholm, M ; Bjerking, G ; Knudsen, J ; Kristiansen, K ; Mandrup, S. / Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein. In: Gene. 1996 ; Vol. 173, No. 2. pp. 233-8.

Bibtex

@article{246665f00fa311de8478000ea68e967b,
title = "Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein",
abstract = "Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing the DR-1 element reduced transcriptional activity, and further indicated that three AP-2 sites and one NF-1/CTF site in the proximal promoter are of importance for basal promoter activity.",
author = "M Elholm and G Bjerking and J Knudsen and K Kristiansen and S Mandrup",
note = "Keywords: Animals; Binding Sites; Carrier Proteins; Diazepam Binding Inhibitor; Electrophoresis, Polyacrylamide Gel; Gene Expression; Phosphoproteins; Promoter Regions, Genetic; Pyrimidines; Rats; Rats, Sprague-Dawley; Transcription Factors; Transfection; Tumor Cells, Cultured",
year = "1996",
doi = "10.1016/0378-1119(96)00213-2",
language = "English",
volume = "173",
pages = "233--8",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Regulatory elements in the promoter region of the rat gene encoding the acyl-CoA-binding protein

AU - Elholm, M

AU - Bjerking, G

AU - Knudsen, J

AU - Kristiansen, K

AU - Mandrup, S

N1 - Keywords: Animals; Binding Sites; Carrier Proteins; Diazepam Binding Inhibitor; Electrophoresis, Polyacrylamide Gel; Gene Expression; Phosphoproteins; Promoter Regions, Genetic; Pyrimidines; Rats; Rats, Sprague-Dawley; Transcription Factors; Transfection; Tumor Cells, Cultured

PY - 1996

Y1 - 1996

N2 - Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing the DR-1 element reduced transcriptional activity, and further indicated that three AP-2 sites and one NF-1/CTF site in the proximal promoter are of importance for basal promoter activity.

AB - Acyl-CoA-binding protein (ACBP) is an ubiquitously expressed 10-kDa protein which is present in high amounts in cells involved in solute transport or secretion. Rat ACBP is encoded by a gene containing the typical hallmarks of a housekeeping gene. Analysis of the promoter region of the rat ACBP gene by electrophoretic mobility shift assay (EMSA) revealed specific binding of proteins from rat liver nuclear extracts to potential recognition sequences of NF-1/CTF, Sp1, AP-1, C/EBP and HNF-3. In addition, specific binding to a DR-1 type element was observed. By using in vitro translated peroxisome proliferator activated receptors (PPAR) and a retinoid X receptor alpha (RXRalpha), we demonstrated that this DR-1 element was capable of binding PPARalpha/RXRalpha, PPARdelta/RXRalpha and PPARgamma2/RXRalpha heterodimers. The PPARgamma2/RXRalpha heterodimer appeared to have the highest affinity for the ACBP DR-1 element. Addition of peroxisome proliferators (PP) to H4IIEC3 rat hepatoma cells led to an increase in the ACBP mRNA level, indicating that the DR-1 element could be a functional peroxisome proliferator responsive element (PPRE). Analysis of the ACBP promoter by transient transfection showed that deletion of the region containing the DR-1 element reduced transcriptional activity, and further indicated that three AP-2 sites and one NF-1/CTF site in the proximal promoter are of importance for basal promoter activity.

U2 - 10.1016/0378-1119(96)00213-2

DO - 10.1016/0378-1119(96)00213-2

M3 - Journal article

C2 - 8964505

VL - 173

SP - 233

EP - 238

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -

ID: 11253698