Ribosome synthesis in Tetrahymena: a quantitative analysis
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Ribosome synthesis in Tetrahymena: a quantitative analysis. / Larsen, L K; Andreasen, P H; Kristiansen, K.
In: Cell Biology International, Vol. 23, No. 11, 1999, p. 729-38.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Ribosome synthesis in Tetrahymena: a quantitative analysis
AU - Larsen, L K
AU - Andreasen, P H
AU - Kristiansen, K
N1 - Keywords: Animals; Cell Nucleus; Kinetics; RNA, Messenger; RNA, Ribosomal; Ribosomal Proteins; Ribosomes; Tetrahymena thermophila; Transcription, Genetic
PY - 1999
Y1 - 1999
N2 - We have performed a detailed quantitative analysis of the transcription and accumulation of ribosomal RNA and ribosomal protein mRNA in the ciliated protozoan Tetrahymena thermophila during changes in growth conditions, and found that: (1) nutritional downshifts lead to a rapid decrease in transcriptional activity whereas nutritional upshifts lead to rapid restoration of transcriptional activity, (2) starvation leads to decreased translation of ribosomal protein mRNA and (3) the rate of ribosomal protein mRNA degradation decreases after a nutritional upshift. We present evidence that the proximal promoters of two ribosomal protein genes and the ribosomal RNA gene compete for binding of nuclear factor(s) in vitro, suggesting that the coordinated regulation of these genes may involve a common set of transcriptional regulators.
AB - We have performed a detailed quantitative analysis of the transcription and accumulation of ribosomal RNA and ribosomal protein mRNA in the ciliated protozoan Tetrahymena thermophila during changes in growth conditions, and found that: (1) nutritional downshifts lead to a rapid decrease in transcriptional activity whereas nutritional upshifts lead to rapid restoration of transcriptional activity, (2) starvation leads to decreased translation of ribosomal protein mRNA and (3) the rate of ribosomal protein mRNA degradation decreases after a nutritional upshift. We present evidence that the proximal promoters of two ribosomal protein genes and the ribosomal RNA gene compete for binding of nuclear factor(s) in vitro, suggesting that the coordinated regulation of these genes may involve a common set of transcriptional regulators.
U2 - 10.1006/cbir.1999.0376
DO - 10.1006/cbir.1999.0376
M3 - Journal article
C2 - 10736197
VL - 23
SP - 729
EP - 738
JO - Cell Biology International
JF - Cell Biology International
SN - 1065-6995
IS - 11
ER -
ID: 11254307