Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation

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Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation. / Holst, Dorte; Luquet, Serge; Kristiansen, Karsten; Grimaldi, Paul A.

In: Experimental Cell Research, Vol. 288, No. 1, 2003, p. 168-76.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Holst, D, Luquet, S, Kristiansen, K & Grimaldi, PA 2003, 'Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation', Experimental Cell Research, vol. 288, no. 1, pp. 168-76. https://doi.org/10.1016/S0014-4827(03)00179-4

APA

Holst, D., Luquet, S., Kristiansen, K., & Grimaldi, P. A. (2003). Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation. Experimental Cell Research, 288(1), 168-76. https://doi.org/10.1016/S0014-4827(03)00179-4

Vancouver

Holst D, Luquet S, Kristiansen K, Grimaldi PA. Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation. Experimental Cell Research. 2003;288(1):168-76. https://doi.org/10.1016/S0014-4827(03)00179-4

Author

Holst, Dorte ; Luquet, Serge ; Kristiansen, Karsten ; Grimaldi, Paul A. / Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation. In: Experimental Cell Research. 2003 ; Vol. 288, No. 1. pp. 168-76.

Bibtex

@article{290113100efb11de8478000ea68e967b,
title = "Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation",
abstract = "Dietary long chain fatty acids and thiazolidinediones act as potent activators of adipogenesis in established preadipose cell lines. High concentrations of thiazolidinediones have also been shown to induce terminal differentiation of non-preadipose cells, such as fibroblasts and myoblasts, into adipose-like cells. This transdifferentiation was observed in both rodent and human myoblasts. In this report, we show that PPARdelta mediates some of the effects exerted by long chain fatty acids on myogenesis and adipogenesis. Activation of PPARdelta by long chain fatty acids impairs the expression of the determination factor MyoD1 and alpha-actin, abolishes the development of multinucleated myotubes, and in parallel induces the expression of PPARgamma gene, a master regulator of adipogenesis. Ectopic expression of PPARdelta in C2C12 myoblasts potentiated the fatty acid-induced expression of adipogenic markers, while expression of a dominant negative PPARdelta mutant exerted opposite effects. Furthermore, a sequential activation of first PPARdelta with long chain fatty acids and then PPARgamma with thiazolidinediones is required for adipogenesis in C2C12 myoblasts. This study demonstrates that PPARdelta, at least in part, is responsible for the dual effects of long chain fatty acids as inhibitors of myogenesis and inducers of transdifferentiation into preadipose-like cells.",
author = "Dorte Holst and Serge Luquet and Karsten Kristiansen and Grimaldi, {Paul A}",
note = "Keywords: Adipocytes; Animals; Cell Differentiation; Cell Line; Fatty Acids; Gene Expression Regulation; Mice; Muscle Development; Mutation; MyoD Protein; Myoblasts; Receptors, Cytoplasmic and Nuclear; Transcription Factors; Transfection",
year = "2003",
doi = "10.1016/S0014-4827(03)00179-4",
language = "English",
volume = "288",
pages = "168--76",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation

AU - Holst, Dorte

AU - Luquet, Serge

AU - Kristiansen, Karsten

AU - Grimaldi, Paul A

N1 - Keywords: Adipocytes; Animals; Cell Differentiation; Cell Line; Fatty Acids; Gene Expression Regulation; Mice; Muscle Development; Mutation; MyoD Protein; Myoblasts; Receptors, Cytoplasmic and Nuclear; Transcription Factors; Transfection

PY - 2003

Y1 - 2003

N2 - Dietary long chain fatty acids and thiazolidinediones act as potent activators of adipogenesis in established preadipose cell lines. High concentrations of thiazolidinediones have also been shown to induce terminal differentiation of non-preadipose cells, such as fibroblasts and myoblasts, into adipose-like cells. This transdifferentiation was observed in both rodent and human myoblasts. In this report, we show that PPARdelta mediates some of the effects exerted by long chain fatty acids on myogenesis and adipogenesis. Activation of PPARdelta by long chain fatty acids impairs the expression of the determination factor MyoD1 and alpha-actin, abolishes the development of multinucleated myotubes, and in parallel induces the expression of PPARgamma gene, a master regulator of adipogenesis. Ectopic expression of PPARdelta in C2C12 myoblasts potentiated the fatty acid-induced expression of adipogenic markers, while expression of a dominant negative PPARdelta mutant exerted opposite effects. Furthermore, a sequential activation of first PPARdelta with long chain fatty acids and then PPARgamma with thiazolidinediones is required for adipogenesis in C2C12 myoblasts. This study demonstrates that PPARdelta, at least in part, is responsible for the dual effects of long chain fatty acids as inhibitors of myogenesis and inducers of transdifferentiation into preadipose-like cells.

AB - Dietary long chain fatty acids and thiazolidinediones act as potent activators of adipogenesis in established preadipose cell lines. High concentrations of thiazolidinediones have also been shown to induce terminal differentiation of non-preadipose cells, such as fibroblasts and myoblasts, into adipose-like cells. This transdifferentiation was observed in both rodent and human myoblasts. In this report, we show that PPARdelta mediates some of the effects exerted by long chain fatty acids on myogenesis and adipogenesis. Activation of PPARdelta by long chain fatty acids impairs the expression of the determination factor MyoD1 and alpha-actin, abolishes the development of multinucleated myotubes, and in parallel induces the expression of PPARgamma gene, a master regulator of adipogenesis. Ectopic expression of PPARdelta in C2C12 myoblasts potentiated the fatty acid-induced expression of adipogenic markers, while expression of a dominant negative PPARdelta mutant exerted opposite effects. Furthermore, a sequential activation of first PPARdelta with long chain fatty acids and then PPARgamma with thiazolidinediones is required for adipogenesis in C2C12 myoblasts. This study demonstrates that PPARdelta, at least in part, is responsible for the dual effects of long chain fatty acids as inhibitors of myogenesis and inducers of transdifferentiation into preadipose-like cells.

U2 - 10.1016/S0014-4827(03)00179-4

DO - 10.1016/S0014-4827(03)00179-4

M3 - Journal article

C2 - 12878168

VL - 288

SP - 168

EP - 176

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -

ID: 11230725