Spatial transcriptome unveils a discontinuous inflammatory pattern in proficient mismatch repair colorectal adenocarcinoma

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  • Rongxin Zhang
  • Yu Feng
  • Wenjuan Ma
  • Yanying Guo
  • Mei Luo
  • Young Li
  • Yupeng Zang
  • Xuan Dong
  • Shixun Lu
  • Qiang Guo
  • Qumiao Xu
  • Huanyi Chen
  • Yijian Li
  • Longqi Liu
  • CHEN, Ao
  • Gong Chen
  • Xun Xu

The preexistence of immune cells in the tumor microenvironment substantiates the efficacy of immunotherapy in cancer patients. Although the complex intratumoral immune heterogeneity has been extensively studied in single cell resolution, hi-res spatial investigations are limited. In this study, we performed a spatial transcriptome analysis of 4 colorectal adenocarcinoma specimens and 2 paired distant normal specimens to identify the molecular pattern involved in a discontinuous inflammatory response in pathologically annotated cancer regions. Based on the location of spatially varied gene expression, we unmasked the spatially-varied immune ecosystem and identified the locoregional “warmed-up” immune response in predefined “cold” tumor with substantial infiltration of immune components. This “warmed-up” immune profile was found to be associated with the in-situ copy number variance and the tissue remodeling process. Further, “warmed-up” signature genes indicated improved overall survival in CRC patients obtained from TCGA database.

Original languageEnglish
JournalFundamental Research
Volume3
Issue number4
Number of pages7
ISSN2667-3258
DOIs
Publication statusPublished - 2023

Bibliographical note

Publisher Copyright:
© 2022

    Research areas

  • Colorectal adenocarcinoma, Inflammation, Mismatch repair, Spatial transcriptomics, Tumor microenvironment

ID: 310558072