The DNA methylome of human peripheral blood mononuclear cells
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The DNA methylome of human peripheral blood mononuclear cells. / Li, Yingrui; Zhu, Jingde; Tian, Geng; Li, Ning; Li, Qibin; Ye, Mingzhi; Zheng, Hancheng; Yu, Jian; Wu, Honglong; Sun, Jihua; Zhang, Hongyu; Chen, Quan; Luo, Ruibang; Chen, Minfeng; He, Yinghua; Jin, Xin; Zhang, Qinghui; Yu, Chang; Zhou, Guangyu; Sun, Jinfeng; Huang, Yebo; Zheng, Huisong; Cao, Hongzhi; Zhou, Xiaoyu; Guo, Shicheng; Hu, Xueda; Li, Xin; Kristiansen, Karsten; Bolund, Lars; Xu, Jiujin; Wang, Wen; Yang, Huanming; Wang, Jian; Li, Ruiqiang; Beck, Stephan; Wang, Jun; Zhang, Xiuqing.
In: PLoS - Biology, Vol. 8, No. 11, 01.01.2010, p. e1000533-e1000533.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The DNA methylome of human peripheral blood mononuclear cells
AU - Li, Yingrui
AU - Zhu, Jingde
AU - Tian, Geng
AU - Li, Ning
AU - Li, Qibin
AU - Ye, Mingzhi
AU - Zheng, Hancheng
AU - Yu, Jian
AU - Wu, Honglong
AU - Sun, Jihua
AU - Zhang, Hongyu
AU - Chen, Quan
AU - Luo, Ruibang
AU - Chen, Minfeng
AU - He, Yinghua
AU - Jin, Xin
AU - Zhang, Qinghui
AU - Yu, Chang
AU - Zhou, Guangyu
AU - Sun, Jinfeng
AU - Huang, Yebo
AU - Zheng, Huisong
AU - Cao, Hongzhi
AU - Zhou, Xiaoyu
AU - Guo, Shicheng
AU - Hu, Xueda
AU - Li, Xin
AU - Kristiansen, Karsten
AU - Bolund, Lars
AU - Xu, Jiujin
AU - Wang, Wen
AU - Yang, Huanming
AU - Wang, Jian
AU - Li, Ruiqiang
AU - Beck, Stephan
AU - Wang, Jun
AU - Zhang, Xiuqing
PY - 2010/1/1
Y1 - 2010/1/1
N2 - DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. PBMC constitute an important source for clinical blood tests world-wide. We found that 68.4% of CpG sites and 80% displayed allele-specific expression (ASE). These data demonstrate that ASM is a recurrent phenomenon and is highly correlated with ASE in human PBMCs. Together with recently reported similar studies, our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies.
AB - DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. PBMC constitute an important source for clinical blood tests world-wide. We found that 68.4% of CpG sites and 80% displayed allele-specific expression (ASE). These data demonstrate that ASM is a recurrent phenomenon and is highly correlated with ASE in human PBMCs. Together with recently reported similar studies, our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies.
KW - Alleles
KW - CpG Islands
KW - DNA Methylation
KW - Haploidy
KW - Humans
KW - Leukocytes, Mononuclear
KW - RNA, Untranslated
KW - Sequence Alignment
U2 - 10.1371/journal.pbio.1000533
DO - 10.1371/journal.pbio.1000533
M3 - Journal article
C2 - 21085693
VL - 8
SP - e1000533-e1000533
JO - PLoS Biology
JF - PLoS Biology
SN - 1544-9173
IS - 11
ER -
ID: 32642195