The Retinoblastoma-Histone Deacetylase 3 Complex Inhibits PPAR¿ and Adipocyte Differentiation
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The retinoblastoma protein (RB) has previously been shown to facilitate adipocyte differentiation by inducing cell cycle arrest and enhancing the transactivation by the adipogenic CCAAT/enhancer binding proteins (C/EBP). We show here that the peroxisome proliferator-activated receptor ¿ (PPAR¿), a nuclear receptor pivotal for adipogenesis, promotes adipocyte differentiation more efficiently in the absence of RB. PPAR¿ and RB were shown to coimmunoprecipitate, and this PPAR¿-RB complex also contains the histone deacetylase HDAC3, thereby attenuating PPAR¿'s capacity to drive gene expression and adipocyte differentiation. Dissociation of the PPAR¿-RB-HDAC3 complex by RB phosphorylation or by inhibition of HDAC activity stimulates adipocyte differentiation. These observations underscore an important function of both RB and HDAC3 in fine-tuning PPAR¿ activity and adipocyte differentiation.
Original language | English |
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Journal | Developmental Cell |
Volume | 3 |
Issue number | 6 |
Pages (from-to) | 903-910 |
ISSN | 1534-5807 |
DOIs | |
Publication status | Published - 2002 |
Externally published | Yes |
ID: 14640168