Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids

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Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids. / Pinilla-Redondo, Rafael; Mayo-Muñoz, David; Russel, Jakob; Garrett, Roger A.; Randau, Lennart; Sørensen, Søren J.; Shah, Shiraz A.

In: Nucleic Acids Research, Vol. 48, No. 4, 2020, p. 2000-2012.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pinilla-Redondo, R, Mayo-Muñoz, D, Russel, J, Garrett, RA, Randau, L, Sørensen, SJ & Shah, SA 2020, 'Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids', Nucleic Acids Research, vol. 48, no. 4, pp. 2000-2012. https://doi.org/10.1093/nar/gkz1197

APA

Pinilla-Redondo, R., Mayo-Muñoz, D., Russel, J., Garrett, R. A., Randau, L., Sørensen, S. J., & Shah, S. A. (2020). Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(4), 2000-2012. https://doi.org/10.1093/nar/gkz1197

Vancouver

Pinilla-Redondo R, Mayo-Muñoz D, Russel J, Garrett RA, Randau L, Sørensen SJ et al. Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Research. 2020;48(4):2000-2012. https://doi.org/10.1093/nar/gkz1197

Author

Pinilla-Redondo, Rafael ; Mayo-Muñoz, David ; Russel, Jakob ; Garrett, Roger A. ; Randau, Lennart ; Sørensen, Søren J. ; Shah, Shiraz A. / Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids. In: Nucleic Acids Research. 2020 ; Vol. 48, No. 4. pp. 2000-2012.

Bibtex

@article{8034966892ee4d419c89ac7c0ca3b9ea,
title = "Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids",
abstract = "CRISPR-Cas systems provide prokaryotes with adaptive immune functions against viruses and other genetic parasites. In contrast to all other types of CRISPR-Cas systems, type IV has remained largely overlooked. Here, we describe a previously uncharted diversity of type IV gene cassettes, primarily encoded by plasmid-like elements from diverse prokaryotic taxa. Remarkably, via a comprehensive analysis of their CRISPR spacer content, these systems were found to exhibit a strong bias towards the targeting of other plasmids. Our data indicate that the functions of type IV systems have diverged from those of other host-related CRISPR-Cas immune systems to adopt a role in mediating conflicts between plasmids. Furthermore, we find evidence for cross-talk between certain type IV and type I CRISPR-Cas systems that co-exist intracellularly, thus providing a simple answer to the enigmatic absence of type IV adaptation modules. Collectively, our results lead to the expansion and reclassification of type IV systems and provide novel insights into the biological function and evolution of these elusive systems.",
author = "Rafael Pinilla-Redondo and David Mayo-Mu{\~n}oz and Jakob Russel and Garrett, {Roger A.} and Lennart Randau and S{\o}rensen, {S{\o}ren J.} and Shah, {Shiraz A.}",
note = "{\textcopyright} The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2020",
doi = "10.1093/nar/gkz1197",
language = "English",
volume = "48",
pages = "2000--2012",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "4",

}

RIS

TY - JOUR

T1 - Type IV CRISPR-Cas systems are highly diverse and involved in competition between plasmids

AU - Pinilla-Redondo, Rafael

AU - Mayo-Muñoz, David

AU - Russel, Jakob

AU - Garrett, Roger A.

AU - Randau, Lennart

AU - Sørensen, Søren J.

AU - Shah, Shiraz A.

N1 - © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2020

Y1 - 2020

N2 - CRISPR-Cas systems provide prokaryotes with adaptive immune functions against viruses and other genetic parasites. In contrast to all other types of CRISPR-Cas systems, type IV has remained largely overlooked. Here, we describe a previously uncharted diversity of type IV gene cassettes, primarily encoded by plasmid-like elements from diverse prokaryotic taxa. Remarkably, via a comprehensive analysis of their CRISPR spacer content, these systems were found to exhibit a strong bias towards the targeting of other plasmids. Our data indicate that the functions of type IV systems have diverged from those of other host-related CRISPR-Cas immune systems to adopt a role in mediating conflicts between plasmids. Furthermore, we find evidence for cross-talk between certain type IV and type I CRISPR-Cas systems that co-exist intracellularly, thus providing a simple answer to the enigmatic absence of type IV adaptation modules. Collectively, our results lead to the expansion and reclassification of type IV systems and provide novel insights into the biological function and evolution of these elusive systems.

AB - CRISPR-Cas systems provide prokaryotes with adaptive immune functions against viruses and other genetic parasites. In contrast to all other types of CRISPR-Cas systems, type IV has remained largely overlooked. Here, we describe a previously uncharted diversity of type IV gene cassettes, primarily encoded by plasmid-like elements from diverse prokaryotic taxa. Remarkably, via a comprehensive analysis of their CRISPR spacer content, these systems were found to exhibit a strong bias towards the targeting of other plasmids. Our data indicate that the functions of type IV systems have diverged from those of other host-related CRISPR-Cas immune systems to adopt a role in mediating conflicts between plasmids. Furthermore, we find evidence for cross-talk between certain type IV and type I CRISPR-Cas systems that co-exist intracellularly, thus providing a simple answer to the enigmatic absence of type IV adaptation modules. Collectively, our results lead to the expansion and reclassification of type IV systems and provide novel insights into the biological function and evolution of these elusive systems.

U2 - 10.1093/nar/gkz1197

DO - 10.1093/nar/gkz1197

M3 - Journal article

C2 - 31879772

VL - 48

SP - 2000

EP - 2012

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 4

ER -

ID: 234078187