UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity. / Madsen, Lise; Pedersen, Lone M; Lillefosse, Haldis Haukaas; Fjaere, Even; Bronstad, Ingeborg; Hao, Qin; Petersen, Rasmus Koefoed; Hallenborg, Philip; Ma, Tao; De Matteis, Rita; Araujo, Pedro; Mercader, Josep; Bonet, M Luisa; Hansen, Jacob B; Cannon, Barbara; Nedergaard, Jan; Wang, Jun; Cinti, Saverio; Voshol, Peter; Døskeland, Stein Ove; Kristiansen, Karsten.

In: PLoS ONE, Vol. 5, No. 6, 30.01.2010, p. 1-13.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Madsen, L, Pedersen, LM, Lillefosse, HH, Fjaere, E, Bronstad, I, Hao, Q, Petersen, RK, Hallenborg, P, Ma, T, De Matteis, R, Araujo, P, Mercader, J, Bonet, ML, Hansen, JB, Cannon, B, Nedergaard, J, Wang, J, Cinti, S, Voshol, P, Døskeland, SO & Kristiansen, K 2010, 'UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity', PLoS ONE, vol. 5, no. 6, pp. 1-13. https://doi.org/10.1371/journal.pone.0011391

APA

Madsen, L., Pedersen, L. M., Lillefosse, H. H., Fjaere, E., Bronstad, I., Hao, Q., Petersen, R. K., Hallenborg, P., Ma, T., De Matteis, R., Araujo, P., Mercader, J., Bonet, M. L., Hansen, J. B., Cannon, B., Nedergaard, J., Wang, J., Cinti, S., Voshol, P., ... Kristiansen, K. (2010). UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity. PLoS ONE, 5(6), 1-13. https://doi.org/10.1371/journal.pone.0011391

Vancouver

Madsen L, Pedersen LM, Lillefosse HH, Fjaere E, Bronstad I, Hao Q et al. UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity. PLoS ONE. 2010 Jan 30;5(6):1-13. https://doi.org/10.1371/journal.pone.0011391

Author

Madsen, Lise ; Pedersen, Lone M ; Lillefosse, Haldis Haukaas ; Fjaere, Even ; Bronstad, Ingeborg ; Hao, Qin ; Petersen, Rasmus Koefoed ; Hallenborg, Philip ; Ma, Tao ; De Matteis, Rita ; Araujo, Pedro ; Mercader, Josep ; Bonet, M Luisa ; Hansen, Jacob B ; Cannon, Barbara ; Nedergaard, Jan ; Wang, Jun ; Cinti, Saverio ; Voshol, Peter ; Døskeland, Stein Ove ; Kristiansen, Karsten. / UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity. In: PLoS ONE. 2010 ; Vol. 5, No. 6. pp. 1-13.

Bibtex

@article{9ba75c60a62d11df928f000ea68e967b,
title = "UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity",
abstract = "BACKGROUND: The uncoupling protein 1 (UCP1) is a hallmark of brown adipocytes and pivotal for cold- and diet-induced thermogenesis. METHODOLOGY/PRINCIPAL FINDINGS: Here we report that cyclooxygenase (COX) activity and prostaglandin E(2) (PGE(2)) are crucially involved in induction of UCP1 expression in inguinal white adipocytes, but not in classic interscapular brown adipocytes. Cold-induced expression of UCP1 in inguinal white adipocytes was repressed in COX2 knockout (KO) mice and by administration of the COX inhibitor indomethacin in wild-type mice. Indomethacin repressed beta-adrenergic induction of UCP1 expression in primary inguinal adipocytes. The use of PGE(2) receptor antagonists implicated EP(4) as a main PGE(2) receptor, and injection of the stable PGE(2) analog (EP(3/4) agonist) 16,16 dm PGE(2) induced UCP1 expression in inguinal white adipose tissue. Inhibition of COX activity attenuated diet-induced UCP1 expression and increased energy efficiency and adipose tissue mass in obesity-resistant mice kept at thermoneutrality. CONCLUSIONS/SIGNIFICANCE: Our findings provide evidence that induction of UCP1 expression in white adipose tissue, but not in classic interscapular brown adipose tissue is dependent on cyclooxygenase activity. Our results indicate that cyclooxygenase-dependent induction of UCP1 expression in white adipose tissues is important for diet-induced thermogenesis providing support for a surprising role of COX activity in the control of energy balance and obesity development.",
author = "Lise Madsen and Pedersen, {Lone M} and Lillefosse, {Haldis Haukaas} and Even Fjaere and Ingeborg Bronstad and Qin Hao and Petersen, {Rasmus Koefoed} and Philip Hallenborg and Tao Ma and {De Matteis}, Rita and Pedro Araujo and Josep Mercader and Bonet, {M Luisa} and Hansen, {Jacob B} and Barbara Cannon and Jan Nedergaard and Jun Wang and Saverio Cinti and Peter Voshol and D{\o}skeland, {Stein Ove} and Karsten Kristiansen",
year = "2010",
month = jan,
day = "30",
doi = "10.1371/journal.pone.0011391",
language = "English",
volume = "5",
pages = "1--13",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity

AU - Madsen, Lise

AU - Pedersen, Lone M

AU - Lillefosse, Haldis Haukaas

AU - Fjaere, Even

AU - Bronstad, Ingeborg

AU - Hao, Qin

AU - Petersen, Rasmus Koefoed

AU - Hallenborg, Philip

AU - Ma, Tao

AU - De Matteis, Rita

AU - Araujo, Pedro

AU - Mercader, Josep

AU - Bonet, M Luisa

AU - Hansen, Jacob B

AU - Cannon, Barbara

AU - Nedergaard, Jan

AU - Wang, Jun

AU - Cinti, Saverio

AU - Voshol, Peter

AU - Døskeland, Stein Ove

AU - Kristiansen, Karsten

PY - 2010/1/30

Y1 - 2010/1/30

N2 - BACKGROUND: The uncoupling protein 1 (UCP1) is a hallmark of brown adipocytes and pivotal for cold- and diet-induced thermogenesis. METHODOLOGY/PRINCIPAL FINDINGS: Here we report that cyclooxygenase (COX) activity and prostaglandin E(2) (PGE(2)) are crucially involved in induction of UCP1 expression in inguinal white adipocytes, but not in classic interscapular brown adipocytes. Cold-induced expression of UCP1 in inguinal white adipocytes was repressed in COX2 knockout (KO) mice and by administration of the COX inhibitor indomethacin in wild-type mice. Indomethacin repressed beta-adrenergic induction of UCP1 expression in primary inguinal adipocytes. The use of PGE(2) receptor antagonists implicated EP(4) as a main PGE(2) receptor, and injection of the stable PGE(2) analog (EP(3/4) agonist) 16,16 dm PGE(2) induced UCP1 expression in inguinal white adipose tissue. Inhibition of COX activity attenuated diet-induced UCP1 expression and increased energy efficiency and adipose tissue mass in obesity-resistant mice kept at thermoneutrality. CONCLUSIONS/SIGNIFICANCE: Our findings provide evidence that induction of UCP1 expression in white adipose tissue, but not in classic interscapular brown adipose tissue is dependent on cyclooxygenase activity. Our results indicate that cyclooxygenase-dependent induction of UCP1 expression in white adipose tissues is important for diet-induced thermogenesis providing support for a surprising role of COX activity in the control of energy balance and obesity development.

AB - BACKGROUND: The uncoupling protein 1 (UCP1) is a hallmark of brown adipocytes and pivotal for cold- and diet-induced thermogenesis. METHODOLOGY/PRINCIPAL FINDINGS: Here we report that cyclooxygenase (COX) activity and prostaglandin E(2) (PGE(2)) are crucially involved in induction of UCP1 expression in inguinal white adipocytes, but not in classic interscapular brown adipocytes. Cold-induced expression of UCP1 in inguinal white adipocytes was repressed in COX2 knockout (KO) mice and by administration of the COX inhibitor indomethacin in wild-type mice. Indomethacin repressed beta-adrenergic induction of UCP1 expression in primary inguinal adipocytes. The use of PGE(2) receptor antagonists implicated EP(4) as a main PGE(2) receptor, and injection of the stable PGE(2) analog (EP(3/4) agonist) 16,16 dm PGE(2) induced UCP1 expression in inguinal white adipose tissue. Inhibition of COX activity attenuated diet-induced UCP1 expression and increased energy efficiency and adipose tissue mass in obesity-resistant mice kept at thermoneutrality. CONCLUSIONS/SIGNIFICANCE: Our findings provide evidence that induction of UCP1 expression in white adipose tissue, but not in classic interscapular brown adipose tissue is dependent on cyclooxygenase activity. Our results indicate that cyclooxygenase-dependent induction of UCP1 expression in white adipose tissues is important for diet-induced thermogenesis providing support for a surprising role of COX activity in the control of energy balance and obesity development.

U2 - 10.1371/journal.pone.0011391

DO - 10.1371/journal.pone.0011391

M3 - Journal article

C2 - 20613988

VL - 5

SP - 1

EP - 13

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

ER -

ID: 21338239