Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics – Biologisk Institut - Københavns Universitet

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Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics

Speaker: Postdoc Mireya Plass, Max-Delbrück Center for Molecular Medicine, Germany

Abstract
Flatworms of the species Schmidtea mediterranea are immortal—adult animals contain a large pool of pluripotent stem cells that continuously differentiate to all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from the transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterize gene expression changes during differentiation and discover cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.