Guillermo Montoya
Professor, forskningschef, Professor
Professor Guillermo Montoya is Research Director and Group Leader at the Protein Structure and Function program at Novo Nordisk Foundation Center for Protein Research (CPR) at University of Copenhagen.
Guillermo Montoya’s research aims to understand basic cellular mechanisms at the atomic level, and he firmly believes that the detailed unravelling of these mechanisms will be essential for future biomedical research.
The approach of the Montoya Group is to use advanced methodology, combining X-ray crystallography and cryo-electron microscopy with cell biology to study the structure and function of macromolecules involved in cell cycle progression, genome integrity and its manipulation.
Key discoveries
Montoya solved the first crystal structure of the 1 MDa TRiC/CCT chaperonin in complex with tubulin, shedding light on the folding mechanism that is essential for cell cycle progression and chromosome segregation. His group has recently provided molecular evidence of how key guardians of genome integrity such as the kinase TLK2 or the XMAP215 microtubule polymerase work to protect the genome and provide faithful cell division.
Montoya is also systematically pursuing the structure-function analysis of endonucleases, which are of great interest because of their applications in genome editing. His seminal work in homing endonucleases has shown that these proteins were amenable of redesign in order to target mutations in human monogenic diseases.
His studies elucidating the structure of CRISPR-Cas12a interference ribonucleoprotein have unveiled the mechanism of recognition, unzipping and catalytic activation in order to cleavage target DNA. This finding has opened new avenues in genome modification for biomedicine and biotechnology.
Udvalgte publikationer
- Udgivet
Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas
Molina, R., Stella, S., Feng, M., Sofos, N., Jauniskis, V., Pozdnyakova, I., López-Méndez, B., She, Q. & Montoya, Guillermo, 2019, I: Nature Communications. 10, 14 s., 4302.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
- Udgivet
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity
Stella, S., Mesa, Pablo, Thomsen, J., Paul, B., Alcón, P., Jensen, S. B., Saligram, B., Moses, M. E., Hatzakis, Nikos & Montoya, Guillermo, 13 dec. 2018, I: Cell. 175, 7, s. 1856-1871.e21 16 s.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
- Udgivet
Molecular basis of Tousled-Like Kinase 2 activation
Mortuza, G. B., Hermida, D., Pedersen, Anna-Kathrine, Segura-Bayona, S., López-Méndez, B., Redondo, P., Rüther, P., Pozdnyakova, I., Garrote, A. M., Muñoz, I. G., Villamor-Payà, M., Jauset, C., Olsen, Jesper Velgaard, Stracker, T. H. & Montoya, Guillermo, 2018, I: Nature Communications. 9, 1, s. 2535 2535.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
- Udgivet
Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing
Stella, S., Alcón, P. & Montoya, Guillermo, nov. 2017, I: Nature Structural and Molecular Biology. 24, 11, s. 882-892 11 s.Publikation: Bidrag til tidsskrift › Review › fagfællebedømt
- Udgivet
Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
Stella, S., Alcón, P. & Montoya, Guillermo, 22 jun. 2017, I: Nature. 546, 7659, s. 559-563 5 s.Publikation: Bidrag til tidsskrift › Letter › fagfællebedømt
- Udgivet
Visualizing phosphodiester-bond hydrolysis by an endonuclease
Molina, R., Stella, S., Redondo, P., Gomez, H., Marcaida, M. J., Orozco, M., Prieto, J. & Montoya, Guillermo, jan. 2015, I: Nature Structural and Molecular Biology. 22, 1, s. 65-72 8 s.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
- Udgivet
XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation
Mortuza, G. B., Cavazza, T., Garcia-Mayoral, M. F., Hermida, D., Peset, I., Pedrero, J. G., Merino, N., Blanco, F. J., Lyngsø, J., Bruix, M., Pedersen, J. S., Vernos, I. & Montoya, Guillermo, 29 sep. 2014, I: Nature Communications. 5, s. 1-12 12 s., 5072.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
Directly from the source: endogenous preparations of molecular machines
Mesa, Pablo, Deniaud, A., Montoya, Guillermo & Schaffitzel, C., jun. 2013, I: Current Opinion in Structural Biology. 23, 3, s. 319-25 7 s.Publikation: Bidrag til tidsskrift › Review › fagfællebedømt
- Udgivet
Crystal structure of the open conformation of the mammalian chaperonin CCT in complex with tubulin
Muñoz, I. G., Yébenes, H., Zhou, M., Mesa, Pablo, Serna, M., Park, A. Y., Bragado-Nilsson, E., Beloso, A., de Cárcer, G., Malumbres, M., Robinson, C. V., Valpuesta, J. M. & Montoya, Guillermo, jan. 2011, I: Nature Structural & Molecular Biology. 18, 1, s. 14-9 6 s.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases
Redondo, P., Prieto, J., Muñoz, I. G., Alibés, A., Stricher, F., Serrano, L., Cabaniols, J., Daboussi, F., Arnould, S., Perez, C., Duchateau, P., Pâques, F., Blanco, F. J. & Montoya, Guillermo, 6 nov. 2008, I: Nature. 456, 7218, s. 107-11 5 s.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
ID: 93716435
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Molecular basis of Tousled-Like Kinase 2 activation
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TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress
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Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites
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