Monitoring of biogenic sulfide is important because acid volatile sulfides (AVS) represent a reactive pool responsible for immobilization of toxic metals. We propose a new sulfide paper sensor method for semiquantitative determination of AVS in which developed color is compared to a reference chart. The method was validated against the ion-selective microelectrode and the purge-and-trap methods. For fieldwork, readings should fall within 1 to 10 μmoles S ^2– . Considering that the volume of soil used ranged between 1 and 16 cm ³ , the corresponding soil sulfides concentration range spans from 0.06 to 10 mmoles S ^2– cm ⁻³ . The sulfide paper sensor method is highly suitable for field screening and has sensitivity levels comparable to laboratory methods. Environ Toxicol Chem 2018;37:3025–3031.