AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research

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Standard

AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research. / Munkager, Victoria; Vestergård, Mette; Priemé, Anders; Altenburger, Andreas; de Visser, Eva; Johansen, Jesper Liengaard; Ekelund, Flemming.

I: Plants, Bind 9, Nr. 3, 372, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Munkager, V, Vestergård, M, Priemé, A, Altenburger, A, de Visser, E, Johansen, JL & Ekelund, F 2020, 'AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research', Plants, bind 9, nr. 3, 372. https://doi.org/10.3390/plants9030372

APA

Munkager, V., Vestergård, M., Priemé, A., Altenburger, A., de Visser, E., Johansen, J. L., & Ekelund, F. (2020). AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research. Plants, 9(3), [372]. https://doi.org/10.3390/plants9030372

Vancouver

Munkager V, Vestergård M, Priemé A, Altenburger A, de Visser E, Johansen JL o.a. AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research. Plants. 2020;9(3). 372. https://doi.org/10.3390/plants9030372

Author

Munkager, Victoria ; Vestergård, Mette ; Priemé, Anders ; Altenburger, Andreas ; de Visser, Eva ; Johansen, Jesper Liengaard ; Ekelund, Flemming. / AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research. I: Plants. 2020 ; Bind 9, Nr. 3.

Bibtex

@article{25e0b5aeb456412ea461c737d2c27c57,
title = "AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research",
abstract = "To understand and manipulate the interactions between plants and microorganisms, sterile seeds are a necessity. The seed microbiome (inside and surface microorganisms) is unknown for most plant species and seed-borne microorganisms can persist and transfer to the seedling and rhizosphere, thereby obscuring the effects that purposely introduced microorganisms have on plants. This necessitates that these unidentified, seed-borne microorganisms are removed before seeds are used for studies on plant–microbiome interactions. Unfortunately, there is no single, standardized protocol for seed sterilization, hampering progress in experimental plant growth promotion and our study shows that commonly applied sterilization protocols for barley grains using H2O2, NaClO, and AgNO3 yielded insufficient sterilization. We therefore developed a sterilization protocol with AgNO3 by testing several concentrations of AgNO3 and added two additional steps: Soaking the grains in water before the sterilization and rinsing with salt water (1% (w/w) NaCl) after the sterilization. The most efficient sterilization protocol was to soak the grains, sterilize with 10% (w/w) AgNO3, and to rinse with salt water. By following those three steps, 97% of the grains had no culturable, viable microorganism after 21 days based on microscopic inspection. The protocol left small quantities of AgNO3 residue on the grain, maintained germination percentage similar to unsterilized grains, and plant biomass was unaltered. Hence, our protocol using AgNO3 can be used successfully for experiments on plant–microbiome interactions.",
keywords = "AgNO, Barley, Endophyte sterilization, Grain sterilization, Hordeum vulgare, Microbiome, Plant, Seed sterilization, Silver nitrate",
author = "Victoria Munkager and Mette Vesterg{\aa}rd and Anders Priem{\'e} and Andreas Altenburger and {de Visser}, Eva and Johansen, {Jesper Liengaard} and Flemming Ekelund",
year = "2020",
doi = "10.3390/plants9030372",
language = "English",
volume = "9",
journal = "Plants",
issn = "2223-7747",
publisher = "MDPI AG",
number = "3",

}

RIS

TY - JOUR

T1 - AgNO3 sterilizes grains of barley (Hordeum vulgare) without inhibiting germination - a necessary tool for plant–microbiome research

AU - Munkager, Victoria

AU - Vestergård, Mette

AU - Priemé, Anders

AU - Altenburger, Andreas

AU - de Visser, Eva

AU - Johansen, Jesper Liengaard

AU - Ekelund, Flemming

PY - 2020

Y1 - 2020

N2 - To understand and manipulate the interactions between plants and microorganisms, sterile seeds are a necessity. The seed microbiome (inside and surface microorganisms) is unknown for most plant species and seed-borne microorganisms can persist and transfer to the seedling and rhizosphere, thereby obscuring the effects that purposely introduced microorganisms have on plants. This necessitates that these unidentified, seed-borne microorganisms are removed before seeds are used for studies on plant–microbiome interactions. Unfortunately, there is no single, standardized protocol for seed sterilization, hampering progress in experimental plant growth promotion and our study shows that commonly applied sterilization protocols for barley grains using H2O2, NaClO, and AgNO3 yielded insufficient sterilization. We therefore developed a sterilization protocol with AgNO3 by testing several concentrations of AgNO3 and added two additional steps: Soaking the grains in water before the sterilization and rinsing with salt water (1% (w/w) NaCl) after the sterilization. The most efficient sterilization protocol was to soak the grains, sterilize with 10% (w/w) AgNO3, and to rinse with salt water. By following those three steps, 97% of the grains had no culturable, viable microorganism after 21 days based on microscopic inspection. The protocol left small quantities of AgNO3 residue on the grain, maintained germination percentage similar to unsterilized grains, and plant biomass was unaltered. Hence, our protocol using AgNO3 can be used successfully for experiments on plant–microbiome interactions.

AB - To understand and manipulate the interactions between plants and microorganisms, sterile seeds are a necessity. The seed microbiome (inside and surface microorganisms) is unknown for most plant species and seed-borne microorganisms can persist and transfer to the seedling and rhizosphere, thereby obscuring the effects that purposely introduced microorganisms have on plants. This necessitates that these unidentified, seed-borne microorganisms are removed before seeds are used for studies on plant–microbiome interactions. Unfortunately, there is no single, standardized protocol for seed sterilization, hampering progress in experimental plant growth promotion and our study shows that commonly applied sterilization protocols for barley grains using H2O2, NaClO, and AgNO3 yielded insufficient sterilization. We therefore developed a sterilization protocol with AgNO3 by testing several concentrations of AgNO3 and added two additional steps: Soaking the grains in water before the sterilization and rinsing with salt water (1% (w/w) NaCl) after the sterilization. The most efficient sterilization protocol was to soak the grains, sterilize with 10% (w/w) AgNO3, and to rinse with salt water. By following those three steps, 97% of the grains had no culturable, viable microorganism after 21 days based on microscopic inspection. The protocol left small quantities of AgNO3 residue on the grain, maintained germination percentage similar to unsterilized grains, and plant biomass was unaltered. Hence, our protocol using AgNO3 can be used successfully for experiments on plant–microbiome interactions.

KW - AgNO

KW - Barley

KW - Endophyte sterilization

KW - Grain sterilization

KW - Hordeum vulgare

KW - Microbiome

KW - Plant

KW - Seed sterilization

KW - Silver nitrate

U2 - 10.3390/plants9030372

DO - 10.3390/plants9030372

M3 - Journal article

C2 - 32192219

AN - SCOPUS:85082474881

VL - 9

JO - Plants

JF - Plants

SN - 2223-7747

IS - 3

M1 - 372

ER -

ID: 239859967