Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Standard

Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells. / Arora, Pankaj; Moll, Janne Marie; Andersen, Daniel; Workman, Christopher Thomas; Williams, Andrew R.; Kristiansen, Karsten; Brix, Susanne.

I: Immunology, Bind 159, Nr. 3, 2020, s. 322-334.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Arora, P, Moll, JM, Andersen, D, Workman, CT, Williams, AR, Kristiansen, K & Brix, S 2020, 'Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells', Immunology, bind 159, nr. 3, s. 322-334. https://doi.org/10.1111/imm.13151

APA

Arora, P., Moll, J. M., Andersen, D., Workman, C. T., Williams, A. R., Kristiansen, K., & Brix, S. (2020). Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells. Immunology, 159(3), 322-334. https://doi.org/10.1111/imm.13151

Vancouver

Arora P, Moll JM, Andersen D, Workman CT, Williams AR, Kristiansen K o.a. Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells. Immunology. 2020;159(3):322-334. https://doi.org/10.1111/imm.13151

Author

Arora, Pankaj ; Moll, Janne Marie ; Andersen, Daniel ; Workman, Christopher Thomas ; Williams, Andrew R. ; Kristiansen, Karsten ; Brix, Susanne. / Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells. I: Immunology. 2020 ; Bind 159, Nr. 3. s. 322-334.

Bibtex

@article{1893d48714b748fe95bea1f7a6c71e9a,
title = "Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells",
abstract = "Dendritic cells (DCs) are essential for generating T‐cell‐based immune responses through sensing of potential inflammatory and metabolic cues in the local environment. However, there is still limited insight into the processes defining the resultant DC phenotype, including the type of early transcriptional changes in pro‐inflammatory cues towards regulatory or type 2 immune‐based cues induced by a variety of exogenous and endogenous molecules. Here we compared the ability of a selected number of molecules to modulate the pro‐inflammatory phenotype of lipopolysaccharide (LPS) and interferon‐γ (IFN‐γ)‐stimulated human monocyte‐derived DCs towards an anti‐inflammatory or regulatory phenotype, including Ascaris suum body fluid [helminth pseudocoelomic fluid (PCF)], the metabolites succinate and butyrate, and the type 2 cytokines thymic stromal lymphopoietin and interleukin‐25. Our data show that helminth PCF and butyrate treatment suppress the T helper type 1 (Th1)‐inducing pro‐inflammatory DC phenotype through induction of different transcriptional programs in DCs. RNA sequencing indicated that helminth PCF treatment strongly inhibited the Th1 and Th17 polarizing ability of LPS + IFN‐γ‐matured DCs by down‐regulating myeloid differentiation primary response gene 88 (MyD88)‐dependent and MyD88‐independent pathways in Toll‐like receptor 4 signaling. By contrast, butyrate treatment had a strong Th1‐inhibiting action, and transcripts encoding important gut barrier defending factors such as IL18, IL1B and CXCL8 were up‐regulated. Collectively, our results further understanding of how compounds from parasites and gut microbiota‐derived butyrate may exert immunomodulatory effects on the host immune system.",
keywords = "Ascaris suum, dendritic cells, type 2 immune response",
author = "Pankaj Arora and Moll, {Janne Marie} and Daniel Andersen and Workman, {Christopher Thomas} and Williams, {Andrew R.} and Karsten Kristiansen and Susanne Brix",
year = "2020",
doi = "10.1111/imm.13151",
language = "English",
volume = "159",
pages = "322--334",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells

AU - Arora, Pankaj

AU - Moll, Janne Marie

AU - Andersen, Daniel

AU - Workman, Christopher Thomas

AU - Williams, Andrew R.

AU - Kristiansen, Karsten

AU - Brix, Susanne

PY - 2020

Y1 - 2020

N2 - Dendritic cells (DCs) are essential for generating T‐cell‐based immune responses through sensing of potential inflammatory and metabolic cues in the local environment. However, there is still limited insight into the processes defining the resultant DC phenotype, including the type of early transcriptional changes in pro‐inflammatory cues towards regulatory or type 2 immune‐based cues induced by a variety of exogenous and endogenous molecules. Here we compared the ability of a selected number of molecules to modulate the pro‐inflammatory phenotype of lipopolysaccharide (LPS) and interferon‐γ (IFN‐γ)‐stimulated human monocyte‐derived DCs towards an anti‐inflammatory or regulatory phenotype, including Ascaris suum body fluid [helminth pseudocoelomic fluid (PCF)], the metabolites succinate and butyrate, and the type 2 cytokines thymic stromal lymphopoietin and interleukin‐25. Our data show that helminth PCF and butyrate treatment suppress the T helper type 1 (Th1)‐inducing pro‐inflammatory DC phenotype through induction of different transcriptional programs in DCs. RNA sequencing indicated that helminth PCF treatment strongly inhibited the Th1 and Th17 polarizing ability of LPS + IFN‐γ‐matured DCs by down‐regulating myeloid differentiation primary response gene 88 (MyD88)‐dependent and MyD88‐independent pathways in Toll‐like receptor 4 signaling. By contrast, butyrate treatment had a strong Th1‐inhibiting action, and transcripts encoding important gut barrier defending factors such as IL18, IL1B and CXCL8 were up‐regulated. Collectively, our results further understanding of how compounds from parasites and gut microbiota‐derived butyrate may exert immunomodulatory effects on the host immune system.

AB - Dendritic cells (DCs) are essential for generating T‐cell‐based immune responses through sensing of potential inflammatory and metabolic cues in the local environment. However, there is still limited insight into the processes defining the resultant DC phenotype, including the type of early transcriptional changes in pro‐inflammatory cues towards regulatory or type 2 immune‐based cues induced by a variety of exogenous and endogenous molecules. Here we compared the ability of a selected number of molecules to modulate the pro‐inflammatory phenotype of lipopolysaccharide (LPS) and interferon‐γ (IFN‐γ)‐stimulated human monocyte‐derived DCs towards an anti‐inflammatory or regulatory phenotype, including Ascaris suum body fluid [helminth pseudocoelomic fluid (PCF)], the metabolites succinate and butyrate, and the type 2 cytokines thymic stromal lymphopoietin and interleukin‐25. Our data show that helminth PCF and butyrate treatment suppress the T helper type 1 (Th1)‐inducing pro‐inflammatory DC phenotype through induction of different transcriptional programs in DCs. RNA sequencing indicated that helminth PCF treatment strongly inhibited the Th1 and Th17 polarizing ability of LPS + IFN‐γ‐matured DCs by down‐regulating myeloid differentiation primary response gene 88 (MyD88)‐dependent and MyD88‐independent pathways in Toll‐like receptor 4 signaling. By contrast, butyrate treatment had a strong Th1‐inhibiting action, and transcripts encoding important gut barrier defending factors such as IL18, IL1B and CXCL8 were up‐regulated. Collectively, our results further understanding of how compounds from parasites and gut microbiota‐derived butyrate may exert immunomodulatory effects on the host immune system.

KW - Ascaris suum

KW - dendritic cells

KW - type 2 immune response

U2 - 10.1111/imm.13151

DO - 10.1111/imm.13151

M3 - Journal article

C2 - 31705653

VL - 159

SP - 322

EP - 334

JO - Immunology

JF - Immunology

SN - 0019-2805

IS - 3

ER -

ID: 233584380