DNA Double-Strand Break-Induced Gene Amplification in Yeast

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Standard

DNA Double-Strand Break-Induced Gene Amplification in Yeast. / Strucko, Tomas; Lisby, Michael; Mortensen, Uffe Hasbro.

Homologous Recombination: Methods and Protocols. red. / Andrés Aguilera; Aura Carreira. Humana Press, 2021. s. 239-252 (Methods in Molecular Biology, Bind 2153).

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Harvard

Strucko, T, Lisby, M & Mortensen, UH 2021, DNA Double-Strand Break-Induced Gene Amplification in Yeast. i A Aguilera & A Carreira (red), Homologous Recombination: Methods and Protocols. Humana Press, Methods in Molecular Biology, bind 2153, s. 239-252. https://doi.org/10.1007/978-1-0716-0644-5_17

APA

Strucko, T., Lisby, M., & Mortensen, U. H. (2021). DNA Double-Strand Break-Induced Gene Amplification in Yeast. I A. Aguilera, & A. Carreira (red.), Homologous Recombination: Methods and Protocols (s. 239-252). Humana Press. Methods in Molecular Biology Bind 2153 https://doi.org/10.1007/978-1-0716-0644-5_17

Vancouver

Strucko T, Lisby M, Mortensen UH. DNA Double-Strand Break-Induced Gene Amplification in Yeast. I Aguilera A, Carreira A, red., Homologous Recombination: Methods and Protocols. Humana Press. 2021. s. 239-252. (Methods in Molecular Biology, Bind 2153). https://doi.org/10.1007/978-1-0716-0644-5_17

Author

Strucko, Tomas ; Lisby, Michael ; Mortensen, Uffe Hasbro. / DNA Double-Strand Break-Induced Gene Amplification in Yeast. Homologous Recombination: Methods and Protocols. red. / Andrés Aguilera ; Aura Carreira. Humana Press, 2021. s. 239-252 (Methods in Molecular Biology, Bind 2153).

Bibtex

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title = "DNA Double-Strand Break-Induced Gene Amplification in Yeast",
abstract = "Precise control of the gene copy number in the model yeast Saccharomyces cerevisiae may facilitate elucidation of enzyme functions or, in cell factory design, can be used to optimize production of proteins and metabolites. Currently, available methods can provide high gene-expression levels but fail to achieve accurate gene dosage. Moreover, strains generated using these methods often suffer from genetic instability resulting in loss of gene copies during prolonged cultivation. Here we present a method, CASCADE, which enables construction of strains with defined gene copy number. With our present system, gene(s) of interest can be amplified up to nine copies, but the upper copy limit of the system can be expanded. Importantly, the resulting strains can be stably propagated in selection-free media.",
keywords = "CEN.PK, DNA double-strand break, Gene amplification, Gene targeting, Homology-directed recombination, I-SceI nuclease, Metabolic engineering, Saccharomyces cerevisiae",
author = "Tomas Strucko and Michael Lisby and Mortensen, {Uffe Hasbro}",
year = "2021",
doi = "10.1007/978-1-0716-0644-5_17",
language = "English",
isbn = "978-1-0716-0643-8",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "239--252",
editor = "Andr{\'e}s Aguilera and Aura Carreira",
booktitle = "Homologous Recombination",
address = "United States",

}

RIS

TY - CHAP

T1 - DNA Double-Strand Break-Induced Gene Amplification in Yeast

AU - Strucko, Tomas

AU - Lisby, Michael

AU - Mortensen, Uffe Hasbro

PY - 2021

Y1 - 2021

N2 - Precise control of the gene copy number in the model yeast Saccharomyces cerevisiae may facilitate elucidation of enzyme functions or, in cell factory design, can be used to optimize production of proteins and metabolites. Currently, available methods can provide high gene-expression levels but fail to achieve accurate gene dosage. Moreover, strains generated using these methods often suffer from genetic instability resulting in loss of gene copies during prolonged cultivation. Here we present a method, CASCADE, which enables construction of strains with defined gene copy number. With our present system, gene(s) of interest can be amplified up to nine copies, but the upper copy limit of the system can be expanded. Importantly, the resulting strains can be stably propagated in selection-free media.

AB - Precise control of the gene copy number in the model yeast Saccharomyces cerevisiae may facilitate elucidation of enzyme functions or, in cell factory design, can be used to optimize production of proteins and metabolites. Currently, available methods can provide high gene-expression levels but fail to achieve accurate gene dosage. Moreover, strains generated using these methods often suffer from genetic instability resulting in loss of gene copies during prolonged cultivation. Here we present a method, CASCADE, which enables construction of strains with defined gene copy number. With our present system, gene(s) of interest can be amplified up to nine copies, but the upper copy limit of the system can be expanded. Importantly, the resulting strains can be stably propagated in selection-free media.

KW - CEN.PK

KW - DNA double-strand break

KW - Gene amplification

KW - Gene targeting

KW - Homology-directed recombination

KW - I-SceI nuclease

KW - Metabolic engineering

KW - Saccharomyces cerevisiae

U2 - 10.1007/978-1-0716-0644-5_17

DO - 10.1007/978-1-0716-0644-5_17

M3 - Book chapter

C2 - 32840784

AN - SCOPUS:85089925285

SN - 978-1-0716-0643-8

T3 - Methods in Molecular Biology

SP - 239

EP - 252

BT - Homologous Recombination

A2 - Aguilera, Andrés

A2 - Carreira, Aura

PB - Humana Press

ER -

ID: 249857300