Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms

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Standard

Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms. / Kang, Dingrong; Huang, Yuhong; Nesme, Joseph; Herschend, Jakob; Jacquiod, Samuel; Kot, Witold; Hansen, Lars Hestbjerg; Lange, Lene; Sørensen, Søren J.

I: Science of the Total Environment, Bind 761, 143281, 2021.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kang, D, Huang, Y, Nesme, J, Herschend, J, Jacquiod, S, Kot, W, Hansen, LH, Lange, L & Sørensen, SJ 2021, 'Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms', Science of the Total Environment, bind 761, 143281. https://doi.org/10.1016/j.scitotenv.2020.143281

APA

Kang, D., Huang, Y., Nesme, J., Herschend, J., Jacquiod, S., Kot, W., ... Sørensen, S. J. (2021). Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms. Science of the Total Environment, 761, [143281]. https://doi.org/10.1016/j.scitotenv.2020.143281

Vancouver

Kang D, Huang Y, Nesme J, Herschend J, Jacquiod S, Kot W o.a. Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms. Science of the Total Environment. 2021;761. 143281. https://doi.org/10.1016/j.scitotenv.2020.143281

Author

Kang, Dingrong ; Huang, Yuhong ; Nesme, Joseph ; Herschend, Jakob ; Jacquiod, Samuel ; Kot, Witold ; Hansen, Lars Hestbjerg ; Lange, Lene ; Sørensen, Søren J. / Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms. I: Science of the Total Environment. 2021 ; Bind 761.

Bibtex

@article{953516739d014cfbab77dcfeb5b285d5,
title = "Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms",
abstract = "Keratin is an insoluble fibrous protein from natural environments, which can be recycled to value-added products by keratinolytic microorganisms. A microbial consortium with efficient keratinolytic activity was previously enriched from soil, but the genetic basis behind its remarkable degradation properties was not investigated yet. To identify the metabolic pathways involved in keratinolysis and clarify the observed synergy among community members, shotgun metagenomic sequencing was performed to reconstruct metagenome-assembled genomes. More than 90{\%} genera of the enriched bacterial consortium was affiliated to Chryseobacterium, Stenotrophomonas, and Pseudomonas. Metabolic potential and putative keratinases were predicted from the metagenomic annotation, providing the genetic basis of keratin degradation. Furthermore, metabolic pathways associated with keratinolytic processes such as amino acid metabolism, disulfide reduction and urea cycle were investigated from seven high-quality metagenome-assembled genomes, revealing the potential metabolic cooperation related to keratin degradation. This knowledge deepens the understanding of microbial keratinolytic mechanisms at play in a complex community, pinpointing the significance of synergistic interactions, which could be further used to optimize industrial keratin degradation processes.",
author = "Dingrong Kang and Yuhong Huang and Joseph Nesme and Jakob Herschend and Samuel Jacquiod and Witold Kot and Hansen, {Lars Hestbjerg} and Lene Lange and S{\o}rensen, {S{\o}ren J.}",
note = "Copyright {\circledC} 2020 Elsevier B.V. All rights reserved.",
year = "2021",
doi = "10.1016/j.scitotenv.2020.143281",
language = "English",
volume = "761",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Metagenomic analysis of a keratin-degrading bacterial consortium provides insight into the keratinolytic mechanisms

AU - Kang, Dingrong

AU - Huang, Yuhong

AU - Nesme, Joseph

AU - Herschend, Jakob

AU - Jacquiod, Samuel

AU - Kot, Witold

AU - Hansen, Lars Hestbjerg

AU - Lange, Lene

AU - Sørensen, Søren J.

N1 - Copyright © 2020 Elsevier B.V. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Keratin is an insoluble fibrous protein from natural environments, which can be recycled to value-added products by keratinolytic microorganisms. A microbial consortium with efficient keratinolytic activity was previously enriched from soil, but the genetic basis behind its remarkable degradation properties was not investigated yet. To identify the metabolic pathways involved in keratinolysis and clarify the observed synergy among community members, shotgun metagenomic sequencing was performed to reconstruct metagenome-assembled genomes. More than 90% genera of the enriched bacterial consortium was affiliated to Chryseobacterium, Stenotrophomonas, and Pseudomonas. Metabolic potential and putative keratinases were predicted from the metagenomic annotation, providing the genetic basis of keratin degradation. Furthermore, metabolic pathways associated with keratinolytic processes such as amino acid metabolism, disulfide reduction and urea cycle were investigated from seven high-quality metagenome-assembled genomes, revealing the potential metabolic cooperation related to keratin degradation. This knowledge deepens the understanding of microbial keratinolytic mechanisms at play in a complex community, pinpointing the significance of synergistic interactions, which could be further used to optimize industrial keratin degradation processes.

AB - Keratin is an insoluble fibrous protein from natural environments, which can be recycled to value-added products by keratinolytic microorganisms. A microbial consortium with efficient keratinolytic activity was previously enriched from soil, but the genetic basis behind its remarkable degradation properties was not investigated yet. To identify the metabolic pathways involved in keratinolysis and clarify the observed synergy among community members, shotgun metagenomic sequencing was performed to reconstruct metagenome-assembled genomes. More than 90% genera of the enriched bacterial consortium was affiliated to Chryseobacterium, Stenotrophomonas, and Pseudomonas. Metabolic potential and putative keratinases were predicted from the metagenomic annotation, providing the genetic basis of keratin degradation. Furthermore, metabolic pathways associated with keratinolytic processes such as amino acid metabolism, disulfide reduction and urea cycle were investigated from seven high-quality metagenome-assembled genomes, revealing the potential metabolic cooperation related to keratin degradation. This knowledge deepens the understanding of microbial keratinolytic mechanisms at play in a complex community, pinpointing the significance of synergistic interactions, which could be further used to optimize industrial keratin degradation processes.

U2 - 10.1016/j.scitotenv.2020.143281

DO - 10.1016/j.scitotenv.2020.143281

M3 - Journal article

C2 - 33190895

VL - 761

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

M1 - 143281

ER -

ID: 251737961