TY - JOUR

T1 - Once in a lifetime

T2 - strategies for preventing re-replication in prokaryotic and eukaryotic cells.

AU - Nielsen, Olaf

AU - Løbner-Olesen, Anders

N1 - Keywords: DNA Replication; Escherichia coli; Eukaryotic Cells; Nucleotides; Prokaryotic Cells; Schizosaccharomyces

PY - 2008

Y1 - 2008

N2 - DNA replication is an extremely accurate process and cells have evolved intricate control mechanisms to ensure that each region of their genome is replicated only once during S phase. Here, we compare what is known about the processes that prevent re-replication in prokaryotic and eukaryotic cells by using the model organisms Escherichia coli and Schizosaccharomyces pombe as examples. Although the underlying molecular details are different, the logic behind the control mechanisms is similar. For example, after initiation, crucial molecules required for the loading of replicative helicases in both prokaryotes and eukaryotes are inactivated until the next cell cycle. Furthermore, in both systems the beta-clamp of the replicative polymerase associates with enzymatic activities that contribute to the inactivation of the helicase loaders. Finally, recent studies suggest that the control mechanism that prevents re-replication in both systems also increases the synthesis of DNA building blocks.

AB - DNA replication is an extremely accurate process and cells have evolved intricate control mechanisms to ensure that each region of their genome is replicated only once during S phase. Here, we compare what is known about the processes that prevent re-replication in prokaryotic and eukaryotic cells by using the model organisms Escherichia coli and Schizosaccharomyces pombe as examples. Although the underlying molecular details are different, the logic behind the control mechanisms is similar. For example, after initiation, crucial molecules required for the loading of replicative helicases in both prokaryotes and eukaryotes are inactivated until the next cell cycle. Furthermore, in both systems the beta-clamp of the replicative polymerase associates with enzymatic activities that contribute to the inactivation of the helicase loaders. Finally, recent studies suggest that the control mechanism that prevents re-replication in both systems also increases the synthesis of DNA building blocks.

U2 - 10.1038/sj.embor.2008.2

DO - 10.1038/sj.embor.2008.2

M3 - Review

C2 - 18246107

VL - 9

SP - 151

EP - 156

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 2

ER -