Organismal benefits of transcription speed control at gene boundaries

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Organismal benefits of transcription speed control at gene boundaries. / Leng, Xueyuan; Ivanov, Maxim; Kindgren, Peter; Malik, Indranil; Thieffry, Axel; Brodersen, Peter; Sandelin, Albin; Kaplan, Craig D.; Marquardt, Sebastian.

I: EMBO Reports, Bind 21, Nr. 4, e49315, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Leng, X, Ivanov, M, Kindgren, P, Malik, I, Thieffry, A, Brodersen, P, Sandelin, A, Kaplan, CD & Marquardt, S 2020, 'Organismal benefits of transcription speed control at gene boundaries', EMBO Reports, bind 21, nr. 4, e49315. https://doi.org/10.15252/embr.201949315

APA

Leng, X., Ivanov, M., Kindgren, P., Malik, I., Thieffry, A., Brodersen, P., Sandelin, A., Kaplan, C. D., & Marquardt, S. (2020). Organismal benefits of transcription speed control at gene boundaries. EMBO Reports, 21(4), [e49315]. https://doi.org/10.15252/embr.201949315

Vancouver

Leng X, Ivanov M, Kindgren P, Malik I, Thieffry A, Brodersen P o.a. Organismal benefits of transcription speed control at gene boundaries. EMBO Reports. 2020;21(4). e49315. https://doi.org/10.15252/embr.201949315

Author

Leng, Xueyuan ; Ivanov, Maxim ; Kindgren, Peter ; Malik, Indranil ; Thieffry, Axel ; Brodersen, Peter ; Sandelin, Albin ; Kaplan, Craig D. ; Marquardt, Sebastian. / Organismal benefits of transcription speed control at gene boundaries. I: EMBO Reports. 2020 ; Bind 21, Nr. 4.

Bibtex

@article{aa2270a0a78045da81efa84c271a34ef,
title = "Organismal benefits of transcription speed control at gene boundaries",
abstract = "RNA polymerase II (RNAPII) transcription is crucial for gene expression. RNAPII density peaks at gene boundaries, associating these key regions for gene expression control with limited RNAPII movement. The connections between RNAPII transcription speed and gene regulation in multicellular organisms are poorly understood. Here, we directly modulate RNAPII transcription speed by point mutations in the second largest subunit of RNAPII in Arabidopsis thaliana. A RNAPII mutation predicted to decelerate transcription is inviable, while accelerating RNAPII transcription confers phenotypes resembling auto-immunity. Nascent transcription profiling revealed that RNAPII complexes with accelerated transcription clear stalling sites at both gene ends, resulting in read-through transcription. The accelerated transcription mutant NRPB2-Y732F exhibits increased association with 5' splice site (5'SS) intermediates and enhanced splicing efficiency. Our findings highlight potential advantages of RNAPII stalling through local reduction in transcription speed to optimize gene expression for the development of multicellular organisms.",
keywords = "NET-seq, speed, splicing, stalling, transcription",
author = "Xueyuan Leng and Maxim Ivanov and Peter Kindgren and Indranil Malik and Axel Thieffry and Peter Brodersen and Albin Sandelin and Kaplan, {Craig D.} and Sebastian Marquardt",
year = "2020",
doi = "10.15252/embr.201949315",
language = "English",
volume = "21",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - Organismal benefits of transcription speed control at gene boundaries

AU - Leng, Xueyuan

AU - Ivanov, Maxim

AU - Kindgren, Peter

AU - Malik, Indranil

AU - Thieffry, Axel

AU - Brodersen, Peter

AU - Sandelin, Albin

AU - Kaplan, Craig D.

AU - Marquardt, Sebastian

PY - 2020

Y1 - 2020

N2 - RNA polymerase II (RNAPII) transcription is crucial for gene expression. RNAPII density peaks at gene boundaries, associating these key regions for gene expression control with limited RNAPII movement. The connections between RNAPII transcription speed and gene regulation in multicellular organisms are poorly understood. Here, we directly modulate RNAPII transcription speed by point mutations in the second largest subunit of RNAPII in Arabidopsis thaliana. A RNAPII mutation predicted to decelerate transcription is inviable, while accelerating RNAPII transcription confers phenotypes resembling auto-immunity. Nascent transcription profiling revealed that RNAPII complexes with accelerated transcription clear stalling sites at both gene ends, resulting in read-through transcription. The accelerated transcription mutant NRPB2-Y732F exhibits increased association with 5' splice site (5'SS) intermediates and enhanced splicing efficiency. Our findings highlight potential advantages of RNAPII stalling through local reduction in transcription speed to optimize gene expression for the development of multicellular organisms.

AB - RNA polymerase II (RNAPII) transcription is crucial for gene expression. RNAPII density peaks at gene boundaries, associating these key regions for gene expression control with limited RNAPII movement. The connections between RNAPII transcription speed and gene regulation in multicellular organisms are poorly understood. Here, we directly modulate RNAPII transcription speed by point mutations in the second largest subunit of RNAPII in Arabidopsis thaliana. A RNAPII mutation predicted to decelerate transcription is inviable, while accelerating RNAPII transcription confers phenotypes resembling auto-immunity. Nascent transcription profiling revealed that RNAPII complexes with accelerated transcription clear stalling sites at both gene ends, resulting in read-through transcription. The accelerated transcription mutant NRPB2-Y732F exhibits increased association with 5' splice site (5'SS) intermediates and enhanced splicing efficiency. Our findings highlight potential advantages of RNAPII stalling through local reduction in transcription speed to optimize gene expression for the development of multicellular organisms.

KW - NET-seq

KW - speed

KW - splicing

KW - stalling

KW - transcription

U2 - 10.15252/embr.201949315

DO - 10.15252/embr.201949315

M3 - Journal article

C2 - 32103605

VL - 21

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 4

M1 - e49315

ER -

ID: 237749444