Organismal benefits of transcription speed control at gene boundaries
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
Standard
Organismal benefits of transcription speed control at gene boundaries. / Leng, Xueyuan; Ivanov, Maxim; Kindgren, Peter; Malik, Indranil; Thieffry, Axel; Brodersen, Peter; Sandelin, Albin; Kaplan, Craig D.; Marquardt, Sebastian.
I: EMBO Reports, Bind 21, Nr. 4, e49315, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Organismal benefits of transcription speed control at gene boundaries
AU - Leng, Xueyuan
AU - Ivanov, Maxim
AU - Kindgren, Peter
AU - Malik, Indranil
AU - Thieffry, Axel
AU - Brodersen, Peter
AU - Sandelin, Albin
AU - Kaplan, Craig D.
AU - Marquardt, Sebastian
PY - 2020
Y1 - 2020
N2 - RNA polymerase II (RNAPII) transcription is crucial for gene expression. RNAPII density peaks at gene boundaries, associating these key regions for gene expression control with limited RNAPII movement. The connections between RNAPII transcription speed and gene regulation in multicellular organisms are poorly understood. Here, we directly modulate RNAPII transcription speed by point mutations in the second largest subunit of RNAPII in Arabidopsis thaliana. A RNAPII mutation predicted to decelerate transcription is inviable, while accelerating RNAPII transcription confers phenotypes resembling auto-immunity. Nascent transcription profiling revealed that RNAPII complexes with accelerated transcription clear stalling sites at both gene ends, resulting in read-through transcription. The accelerated transcription mutant NRPB2-Y732F exhibits increased association with 5' splice site (5'SS) intermediates and enhanced splicing efficiency. Our findings highlight potential advantages of RNAPII stalling through local reduction in transcription speed to optimize gene expression for the development of multicellular organisms.
AB - RNA polymerase II (RNAPII) transcription is crucial for gene expression. RNAPII density peaks at gene boundaries, associating these key regions for gene expression control with limited RNAPII movement. The connections between RNAPII transcription speed and gene regulation in multicellular organisms are poorly understood. Here, we directly modulate RNAPII transcription speed by point mutations in the second largest subunit of RNAPII in Arabidopsis thaliana. A RNAPII mutation predicted to decelerate transcription is inviable, while accelerating RNAPII transcription confers phenotypes resembling auto-immunity. Nascent transcription profiling revealed that RNAPII complexes with accelerated transcription clear stalling sites at both gene ends, resulting in read-through transcription. The accelerated transcription mutant NRPB2-Y732F exhibits increased association with 5' splice site (5'SS) intermediates and enhanced splicing efficiency. Our findings highlight potential advantages of RNAPII stalling through local reduction in transcription speed to optimize gene expression for the development of multicellular organisms.
KW - NET-seq
KW - speed
KW - splicing
KW - stalling
KW - transcription
U2 - 10.15252/embr.201949315
DO - 10.15252/embr.201949315
M3 - Journal article
C2 - 32103605
VL - 21
JO - E M B O Reports
JF - E M B O Reports
SN - 1469-221X
IS - 4
M1 - e49315
ER -
ID: 237749444