Overproduction of DnaA protein stimulates initiation of chromosome and minichromosome replication in Escherichia coli
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Overproduction of DnaA protein stimulates initiation of chromosome and minichromosome replication in Escherichia coli. / Atlung, T; Løbner-Olesen, A; Hansen, F G.
I: Molecular General Genetics, Bind 206, Nr. 1, 01.1987, s. 51-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Overproduction of DnaA protein stimulates initiation of chromosome and minichromosome replication in Escherichia coli
AU - Atlung, T
AU - Løbner-Olesen, A
AU - Hansen, F G
PY - 1987/1
Y1 - 1987/1
N2 - Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an increase in chromosomal gene dosage of oriC proximal DNA. The additional replication forks which were initiated on the chromosome did not lead to an overall increase in DNA content. DNA/DNA hybridization showed an amplification encompassing less than a few hundred kilobases on each side of oriC. Kinetic studies showed that the overinitiation occurred very rapidly after the induction, and that the initiation frequency then decreased to a near normal frequency per oriC. The results indicate that the DnaA protein is one important factor in regulation of initiation of DNA replication from oriC.
AB - Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an increase in chromosomal gene dosage of oriC proximal DNA. The additional replication forks which were initiated on the chromosome did not lead to an overall increase in DNA content. DNA/DNA hybridization showed an amplification encompassing less than a few hundred kilobases on each side of oriC. Kinetic studies showed that the overinitiation occurred very rapidly after the induction, and that the initiation frequency then decreased to a near normal frequency per oriC. The results indicate that the DnaA protein is one important factor in regulation of initiation of DNA replication from oriC.
KW - Bacterial Proteins/genetics
KW - Chromosomes, Bacterial/physiology
KW - DNA Replication
KW - DNA Restriction Enzymes
KW - Escherichia coli/genetics
KW - Gene Amplification
KW - Genes
KW - Genes, Bacterial
KW - Plasmids
M3 - Journal article
C2 - 3033441
VL - 206
SP - 51
EP - 59
JO - Molecular General Genetics
JF - Molecular General Genetics
SN - 0026-8925
IS - 1
ER -
ID: 200973552