The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
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The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity. / Gao, Chun-Hui; Cao, Hui; Cai, Peng; Sørensen, Søren J.
I: ISME Journal, Bind 15, 2021, s. 29-40.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity
AU - Gao, Chun-Hui
AU - Cao, Hui
AU - Cai, Peng
AU - Sørensen, Søren J.
PY - 2021
Y1 - 2021
N2 - Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we established a simple coculture system with two model bacteria in various carbon sources and investigated the influence of initial inoculum ratios of 1:1000 to 1000:1 on community structure, function, and bacterial interaction. We found that the final ratio of the cocultures with different initial inoculum ratios differed in approximately five-sixths of the carbon sources, suggesting that the final ratio is highly dependent on the initial inoculum ratio, while the carbon source preferences of bacteria could not predict the final ratio of cocultures. Furthermore, we found that the initial ratio could regulate the metabolic capacity of the coculture, as only cocultures with initial ratios of 1:1 and 1000:1 gained high capacity on 14 specific carbon sources. The underlying reason may be that the pattern of species interaction is changed by the initial ratio. In conclusion, we showed that the initial ratio can induce emergent properties in coculture. These findings suggest that the initial ratio not only impacts the reproducibility of coculture experiments but also can influence our understanding of generic microbial ecology.
AB - Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we established a simple coculture system with two model bacteria in various carbon sources and investigated the influence of initial inoculum ratios of 1:1000 to 1000:1 on community structure, function, and bacterial interaction. We found that the final ratio of the cocultures with different initial inoculum ratios differed in approximately five-sixths of the carbon sources, suggesting that the final ratio is highly dependent on the initial inoculum ratio, while the carbon source preferences of bacteria could not predict the final ratio of cocultures. Furthermore, we found that the initial ratio could regulate the metabolic capacity of the coculture, as only cocultures with initial ratios of 1:1 and 1000:1 gained high capacity on 14 specific carbon sources. The underlying reason may be that the pattern of species interaction is changed by the initial ratio. In conclusion, we showed that the initial ratio can induce emergent properties in coculture. These findings suggest that the initial ratio not only impacts the reproducibility of coculture experiments but also can influence our understanding of generic microbial ecology.
KW - MULTISPECIES BIOFILM FORMATION
KW - MICROBIAL COMMUNITY
KW - ESCHERICHIA-COLI
KW - COEXISTENCE
KW - COMPETITION
U2 - 10.1038/s41396-020-00751-7
DO - 10.1038/s41396-020-00751-7
M3 - Journal article
C2 - 32887945
VL - 15
SP - 29
EP - 40
JO - I S M E Journal
JF - I S M E Journal
SN - 1751-7362
ER -
ID: 248891483