Anti-viral immune system CRISPR-Cas type I-D working mechanism

Hovedområde:Molecular biology
Målgruppe:Biochemistry, Biology, Molecular Biomedicine

The prokaryotic adaptive anti-viral immune system, Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and their associated Cas proteins, constitutes one of the hottest research topics recently. The basic mechanistic aspects and the application power of the systems received huge attention in Microbiology field as well as in other biological disciplines.

The diverse CRISPR systems have been divided into six types, I to IV, with each subdivided into a number of subtypes. Among type I, the type I-A and type I-E have been studied most extensively, but little is known about subtype ID. We have obtained some preliminary results on type ID system in the hyperthermphilic archaeon Sulfolobus, which need to be further investigated.

It is the lab's interest to understand the working mechanism of CRISPR type I-D system and the exact aim of the Master's project is available upon face to face meeting.

Anvendte metoder:protein purification using FPLC or simpler methods, DNA technology such as cloning, PCR and plasmid purification, virology techniques, genetics
Keywords:anti-viral immune system, CRISPR-Cas
Vejleder(e): Xu Peng