How epithelia are built? Understanding the mechanisms governing epithelial morphogenesis
|Målgruppe:||Biochemistry, Biology, Molecular Biomedicine|
Working place: Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), Mechanics of Tissue Homeostasis (Sedzinski) Lab
The Mechanics of Tissue Homeostasis lab is deeply interested in the molecular mechanisms and forces shaping tissue development. Our lab is a diverse team of cell biologists, physicists and computer scientists, fostering a unique and multidisciplinary environment.
Summary of work program:
Epithelia play many different key roles throughout animal development, being responsible for segregating compartments with different biological characteristics and maintaining homeostasis 1. In order to perform the wide variety of functions they are responsible for during development and homeostasis, certain epithelia must specialize and acquire a unique set of features. One strategy that promotes such specification relies on the movement of specialized precursor cells between different tissue layers, in a process named radial intercalation (RI)2. Interestingly, RI drives the morphogenesis and renewal of many tissues, such as the olfactory, airway, cornea and prostate epithelia among others3. We are using the Xenopus (frog) mucociliary epithelium (MCE) as a model combined with live-imaging microscopy and advanced image analysis to study RI in vivo.
We are looking for students doing their MSc in Molecular Biology, Biochemistry or similar fields and interested in doing their master thesis in our lab. Previously experience in microscopy and image analysis is highly valued but it is not required.
1. Rodriguez-Boulan, E. & Macara, I. G. Organization and execution of the epithelial polarity programme. Nat. Rev. Mol. Cell Biol. 15, 225–42 (2014).
2. Walck-Shannon, E. & Hardin, J. Cell intercalation from top to bottom. Nat. Rev. Mol. Cell Biol. 15, 34–48 (2014).
3. Sedzinski, J., Hannezo, E., Tu, F., Biro, M. & Wallingford, J. B. Emergence of an Apical Epithelial Cell Surface In Vivo. Dev. Cell 36, 24–35 (2016).
|Anvendte metoder:||Xenopus (frog) mucociliary epithelium (MCE) as a model combined with live-imaging microscopy and advanced image analysis to study RI in vivo|
|Vejleder(e):||Assoc. Prof. Jakub Sedzinski|