Molecular mechanisms underlying cilia-related kidney disease

Hovedområde:Cell biology
 
Målgruppe:Molecular Biomedicine, Biochemistry, Biology
 
Niveau:Masters
 
Projektbeskrivelse:

Primary cilia are antenna-like signalling organelles present of the surface of most cells in our body. Mutations in genes that affect cilia formation or function result in severe diseases called ciliopathies, for which there are currently no cures. Our group is interested in the basic molecular mechanisims by which cilia are assembled and function to control signaling, as well as the molecular basis for ciliopathies. One of the most common ciliopathies is Autosomal Dominant Polycystic Kidney Disease (ADPKD), which is caused by mutations in the PKD1 or PKD2 genes encoding the membrane proteins polycystin-1 and polycystin-2, respectively. The polycystins are thought to form a receptor-channel complex that is concentrated in the ciliary membrane, but the mechanisms involved in regulating ciliary transport and homeostasis of the polycystins are not fully understood. In an ongoing study, funded by the Novo Nordisk Foundation, we have identified two new regulators of ciliary polycystin transport and homeostasis. Currently we are using a variety of molecular, biochemical and live cell imaging approaches to investigate the underlying mechanisms, and are looking for talented and motivated MSc students to join our team. If you are interested please write an e-mail to lbpedersen@bio.ku.dk to set up an informal meeting to discuss further.

 
Anvendte metoder:Cell cultures, siRNA/shRNA-mediated gene silencing, immunofluorescence microscopy, live cell imaging, SDS-PAGE and immunoblotting, immunoprecipitation, PCR and cloning
 
Keywords:cilia, signaling, kidney disease, mammalian cells, live cell imaging
 
Vejleder(e): Lotte Bang Pedersen
 
E-mail:lbpedersen@bio.ku.dk