Combining CRISPR-based genome editing and synthetic biology for efficient genetic engineering

Hovedområde:Molecular biology
Målgruppe:Biochemistry, Molecular Biomedicine, Biology

CRISPR-based technology has been used to modify genomes of model organisms including yeast, plants and human cells. Some organisms are, however, difficult to be engineered due to long doubling time. In this project we aim to explore the combination of CRISPR tools, synthetic biology and virology for genome editing of hyperthemophilic archaeon Sulfolobus which has a doubling time of > 12 hours. The project involves a diverse set of techniques and will be a good learning process

Anvendte metoder:CRISPR-based genome editing, cloning, virology, synthetic biology
Vejleder(e): Xu Peng