Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle
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Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle. / Coffey, Vernon G; Pilegaard, Henriette; Garnham, Andrew P; O'Brien, Brendan J; Hawley, John A.
In: Journal of Applied Physiology, Vol. 106, No. 4, 2009, p. 1187-97.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle
AU - Coffey, Vernon G
AU - Pilegaard, Henriette
AU - Garnham, Andrew P
AU - O'Brien, Brendan J
AU - Hawley, John A
N1 - Keywords: Adult; Anaerobic Threshold; Bicycling; Blood Glucose; Blotting, Western; Cross-Over Studies; Diet; Exercise; Glycogen; Humans; Lactic Acid; Male; Muscle Contraction; Muscle Strength; Muscle, Skeletal; Physical Endurance; Physical Fitness; RNA; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Weight Lifting; Young Adult
PY - 2009
Y1 - 2009
N2 - We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 +/- 6.3 yr, body mass of 73.2 +/- 4.5 kg, peak O(2) uptake (Vo(2peak)) of 54.0 +/- 5.7 ml.kg(-1) x min(-1)] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% Vo(2peak)) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1(Ser473) were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling ( approximately 55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise ( approximately 52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling ( approximately 45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference."
AB - We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 +/- 6.3 yr, body mass of 73.2 +/- 4.5 kg, peak O(2) uptake (Vo(2peak)) of 54.0 +/- 5.7 ml.kg(-1) x min(-1)] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% Vo(2peak)) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1(Ser473) were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling ( approximately 55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise ( approximately 52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling ( approximately 45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference."
U2 - 10.1152/japplphysiol.91221.2008
DO - 10.1152/japplphysiol.91221.2008
M3 - Journal article
C2 - 19164772
VL - 106
SP - 1187
EP - 1197
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 4
ER -
ID: 21360279