DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes
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- DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes
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BACKGROUND: Initiation of chromosome replication in E. coli requires the DnaA and DnaC proteins and conditionally-lethal dnaA and dnaC mutants are often used to synchronize cell populations.
METHODOLOGY/PRINCIPAL FINDINGS: DNA microarrays were used to measure mRNA steady-state levels in initiation-deficient dnaA46 and dnaC2 bacteria at permissive and non-permissive temperatures and their expression profiles were compared to MG1655 wildtype cells. For both mutants there was altered expression of genes involved in nucleotide biosynthesis at the non-permissive temperature. Transcription of the dnaA and dnaC genes was increased at the non-permissive temperature in the respective mutant strains indicating auto-regulation of both genes. Induction of the SOS regulon was observed in dnaC2 cells at 38 degrees C and 42 degrees C. Flow cytometric analysis revealed that dnaC2 mutant cells at non-permissive temperature had completed the early stages of chromosome replication initiation.
CONCLUSION/SIGNIFICANCE: We suggest that in dnaC2 cells the SOS response is triggered by persistent open-complex formation at oriC and/or by arrested forks that require DnaC for replication restart.
Original language | English |
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Article number | e2984 |
Journal | P L o S One |
Volume | 3 |
Issue number | 8 |
Number of pages | 8 |
ISSN | 1932-6203 |
DOIs | |
Publication status | Published - 2008 |
Externally published | Yes |
- Bacterial Proteins/genetics, Chromosomes, Bacterial/genetics, DNA Replication/genetics, DNA-Binding Proteins/genetics, Escherichia coli K12/genetics, Escherichia coli Proteins/antagonists & inhibitors, Flow Cytometry, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Genes, Bacterial, Genes, Lethal, Heat-Shock Proteins/genetics, Nucleotides/biosynthesis, Oligonucleotide Array Sequence Analysis, RNA, Bacterial/genetics, RNA, Messenger/genetics, SOS Response (Genetics), Thermodynamics, Transcription, Genetic
Research areas
ID: 200971647