High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein

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Standard

High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein. / Maulucci, Giuseppe; Labate, Valentina; Mele, Marina; Panieri, Emiliano; Arcovito, Giuseppe; Galeotti, Tommaso; Østergaard, Henrik; Winther, Jakob R; De Spirito, Marco; Pani, Giovambattista.

In: Science Signaling, Vol. 1, No. 43, 2008, p. pl3 1-15.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Maulucci, G, Labate, V, Mele, M, Panieri, E, Arcovito, G, Galeotti, T, Østergaard, H, Winther, JR, De Spirito, M & Pani, G 2008, 'High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein', Science Signaling, vol. 1, no. 43, pp. pl3 1-15. https://doi.org/10.1126/scisignal.143pl3

APA

Maulucci, G., Labate, V., Mele, M., Panieri, E., Arcovito, G., Galeotti, T., Østergaard, H., Winther, J. R., De Spirito, M., & Pani, G. (2008). High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein. Science Signaling, 1(43), pl3 1-15. https://doi.org/10.1126/scisignal.143pl3

Vancouver

Maulucci G, Labate V, Mele M, Panieri E, Arcovito G, Galeotti T et al. High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein. Science Signaling. 2008;1(43):pl3 1-15. https://doi.org/10.1126/scisignal.143pl3

Author

Maulucci, Giuseppe ; Labate, Valentina ; Mele, Marina ; Panieri, Emiliano ; Arcovito, Giuseppe ; Galeotti, Tommaso ; Østergaard, Henrik ; Winther, Jakob R ; De Spirito, Marco ; Pani, Giovambattista. / High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein. In: Science Signaling. 2008 ; Vol. 1, No. 43. pp. pl3 1-15.

Bibtex

@article{b5418580c78911dd9473000ea68e967b,
title = "High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein",
abstract = "We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time {"}redox maps{"} of adherent cells and to score subtle changes in the intracellular redox state, such as those induced by overexpression of redox-active proteins. This strategy for in vivo imaging of redox signaling circumvents many of the technical limitations currently encountered in the study of complex redox-based phenomena and promises to contribute substantially to this expanding area of signal transduction.",
author = "Giuseppe Maulucci and Valentina Labate and Marina Mele and Emiliano Panieri and Giuseppe Arcovito and Tommaso Galeotti and Henrik {\O}stergaard and Winther, {Jakob R} and {De Spirito}, Marco and Giovambattista Pani",
year = "2008",
doi = "10.1126/scisignal.143pl3",
language = "English",
volume = "1",
pages = "pl3 1--15",
journal = "Science Signaling",
issn = "1945-0877",
publisher = "American Association for the Advancement of Science",
number = "43",

}

RIS

TY - JOUR

T1 - High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein

AU - Maulucci, Giuseppe

AU - Labate, Valentina

AU - Mele, Marina

AU - Panieri, Emiliano

AU - Arcovito, Giuseppe

AU - Galeotti, Tommaso

AU - Østergaard, Henrik

AU - Winther, Jakob R

AU - De Spirito, Marco

AU - Pani, Giovambattista

PY - 2008

Y1 - 2008

N2 - We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time "redox maps" of adherent cells and to score subtle changes in the intracellular redox state, such as those induced by overexpression of redox-active proteins. This strategy for in vivo imaging of redox signaling circumvents many of the technical limitations currently encountered in the study of complex redox-based phenomena and promises to contribute substantially to this expanding area of signal transduction.

AB - We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time "redox maps" of adherent cells and to score subtle changes in the intracellular redox state, such as those induced by overexpression of redox-active proteins. This strategy for in vivo imaging of redox signaling circumvents many of the technical limitations currently encountered in the study of complex redox-based phenomena and promises to contribute substantially to this expanding area of signal transduction.

U2 - 10.1126/scisignal.143pl3

DO - 10.1126/scisignal.143pl3

M3 - Journal article

C2 - 18957692

VL - 1

SP - pl3 1-15

JO - Science Signaling

JF - Science Signaling

SN - 1945-0877

IS - 43

ER -

ID: 9067092