Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli. / Løbner-Olesen, A; Marinus, M G.

In: Journal of Bacteriology, Vol. 174, No. 2, 01.1992, p. 525-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Løbner-Olesen, A & Marinus, MG 1992, 'Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli', Journal of Bacteriology, vol. 174, no. 2, pp. 525-9.

APA

Løbner-Olesen, A., & Marinus, M. G. (1992). Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli. Journal of Bacteriology, 174(2), 525-9.

Vancouver

Løbner-Olesen A, Marinus MG. Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli. Journal of Bacteriology. 1992 Jan;174(2):525-9.

Author

Løbner-Olesen, A ; Marinus, M G. / Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 2. pp. 525-9.

Bibtex

@article{c8e3d040edf649beaff8e6b0b2c2af22,
title = "Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli",
abstract = "DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.",
keywords = "Amino Acid Sequence, Base Sequence, Chromosomes, Bacterial, Cloning, Molecular, DNA Transposable Elements, Escherichia coli/enzymology, Genes, Bacterial, Lac Operon, Molecular Sequence Data, Mutation, Open Reading Frames, Phosphotransferases/genetics, Phosphotransferases (Alcohol Group Acceptor), Transcription, Genetic",
author = "A L{\o}bner-Olesen and Marinus, {M G}",
year = "1992",
month = jan,
language = "English",
volume = "174",
pages = "525--9",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli

AU - Løbner-Olesen, A

AU - Marinus, M G

PY - 1992/1

Y1 - 1992/1

N2 - DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.

AB - DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Chromosomes, Bacterial

KW - Cloning, Molecular

KW - DNA Transposable Elements

KW - Escherichia coli/enzymology

KW - Genes, Bacterial

KW - Lac Operon

KW - Molecular Sequence Data

KW - Mutation

KW - Open Reading Frames

KW - Phosphotransferases/genetics

KW - Phosphotransferases (Alcohol Group Acceptor)

KW - Transcription, Genetic

M3 - Journal article

C2 - 1309529

VL - 174

SP - 525

EP - 529

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 2

ER -

ID: 200973058