Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals
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Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals. / Christensen, Søren Tvorup; Quie, H; Kemp, K; Rasmussen, L.
In: Cell Biology International, Vol. 20, No. 6, 1996, p. 437-44.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals
AU - Christensen, Søren Tvorup
AU - Quie, H
AU - Kemp, K
AU - Rasmussen, L.
N1 - Keywords: Animals; Cattle; Cell Division; Culture Media; Dose-Response Relationship, Drug; Hemin; Humans; Insulin; Recombinant Proteins; Signal Transduction; Tetrahymena thermophila
PY - 1996
Y1 - 1996
N2 - Cells of Tetrahymena may produce autocrine signal molecules with effects on survival and proliferation. Here we have tested the effects of human recombinant and bovine insulin, and the B22-B30 fragment of bovine insulin over a wide range of concentrations (10(-5)-10(-18) M) on cell survival and proliferation in a synthetic nutrient medium. The cells were grown in conical flasks at low initial cell densities (40 and 400 cells/ml). Insulin prevented rapid cell death and/or promoted cell proliferation over two separate concentration ranges: down to nanomolar levels and again in the low pico- and femtomolar range. At an initial population density of 400 cells/ml the cells multiplied at both concentration intervals. At 40 or fewer organisms/ml the cells multiplied in the high concentration interval, whereas in the low interval they survived for about four times longer than those in the control cultures. B22-B30 added to cultures of 40 initial cells/ml produced a stimulation of cell survival in the low pico- and high femtomolar range. In the presence of hemin (50 nM) cells at 400 initial organisms/ml multiplied at insulin concentrations down to about 3 nM and again from 300 am to 10 pM. In some cases, hemin plus insulin activated cell proliferation between the two concentration intervals as well. At 40 cells/ml the cells not only survived but proliferated in the femtomolar range. Cells in cultures supplemented with both hemin and B22-B30 multiplied at the low concentration interval (from about 100 fM to 10 pM).
AB - Cells of Tetrahymena may produce autocrine signal molecules with effects on survival and proliferation. Here we have tested the effects of human recombinant and bovine insulin, and the B22-B30 fragment of bovine insulin over a wide range of concentrations (10(-5)-10(-18) M) on cell survival and proliferation in a synthetic nutrient medium. The cells were grown in conical flasks at low initial cell densities (40 and 400 cells/ml). Insulin prevented rapid cell death and/or promoted cell proliferation over two separate concentration ranges: down to nanomolar levels and again in the low pico- and femtomolar range. At an initial population density of 400 cells/ml the cells multiplied at both concentration intervals. At 40 or fewer organisms/ml the cells multiplied in the high concentration interval, whereas in the low interval they survived for about four times longer than those in the control cultures. B22-B30 added to cultures of 40 initial cells/ml produced a stimulation of cell survival in the low pico- and high femtomolar range. In the presence of hemin (50 nM) cells at 400 initial organisms/ml multiplied at insulin concentrations down to about 3 nM and again from 300 am to 10 pM. In some cases, hemin plus insulin activated cell proliferation between the two concentration intervals as well. At 40 cells/ml the cells not only survived but proliferated in the femtomolar range. Cells in cultures supplemented with both hemin and B22-B30 multiplied at the low concentration interval (from about 100 fM to 10 pM).
U2 - 10.1006/cbir.1996.0055
DO - 10.1006/cbir.1996.0055
M3 - Journal article
C2 - 8858828
VL - 20
SP - 437
EP - 444
JO - Cell Biology International
JF - Cell Biology International
SN - 1065-6995
IS - 6
ER -
ID: 11256053