Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading. / Sørensen, Michael Askvad; Elf, J.; Bouakaz, E.; Tenson, T.; Sanyal, S.; Björk, G. R.; Ehrenberg, M.

In: Journal of Molecular Biology, Vol. 354, No. 1, 2005, p. 16-24.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sørensen, MA, Elf, J, Bouakaz, E, Tenson, T, Sanyal, S, Björk, GR & Ehrenberg, M 2005, 'Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading', Journal of Molecular Biology, vol. 354, no. 1, pp. 16-24. https://doi.org/10.1016/j.jmb.2005.08.076

APA

Sørensen, M. A., Elf, J., Bouakaz, E., Tenson, T., Sanyal, S., Björk, G. R., & Ehrenberg, M. (2005). Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading. Journal of Molecular Biology, 354(1), 16-24. https://doi.org/10.1016/j.jmb.2005.08.076

Vancouver

Sørensen MA, Elf J, Bouakaz E, Tenson T, Sanyal S, Björk GR et al. Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading. Journal of Molecular Biology. 2005;354(1):16-24. https://doi.org/10.1016/j.jmb.2005.08.076

Author

Sørensen, Michael Askvad ; Elf, J. ; Bouakaz, E. ; Tenson, T. ; Sanyal, S. ; Björk, G. R. ; Ehrenberg, M. / Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading. In: Journal of Molecular Biology. 2005 ; Vol. 354, No. 1. pp. 16-24.

Bibtex

@article{d94644a074c211dbbee902004c4f4f50,
title = "Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading",
abstract = "During mRNA translation, synonymous codons for one amino acid areoften read by different isoaccepting tRNAs. The theory of selective tRNAcharging predicts greatly varying percentages of aminoacylation amongisoacceptors in cells starved for their common amino acid. It also predictsmajor changes in tRNA charging patterns upon concentration changes ofsingle isoacceptors, which suggests a novel type of translational control ofgene expression. We therefore tested the theory by measuring withNorthern blots the charging of Leu-tRNAs in Escherichia coli under Leulimitation in response to over expression of tRNALeuGAG. As predicted, thecharged level of tRNALeuGAG increased and the charged levels of four otherLeu isoacceptors decreased or remained unchanged, but the charged levelof tRNALeuUAG increased unexpectedly. To remove this apparent inconsistencybetween theory and experiment we postulated a previouslyunknown common codon for tRNALeuGAG and tRNALeuUAG. Subsequently, wedemonstrated that the tRNALeuGAG codon CUU is, in fact, read also bytRNALeuUAG, due to a uridine-5-oxyacetic acid modification.",
author = "S{\o}rensen, {Michael Askvad} and J. Elf and E. Bouakaz and T. Tenson and S. Sanyal and Bj{\"o}rk, {G. R.} and M. Ehrenberg",
note = "Keywords: aminoacylation; systems biology; decoding; staruation; kinetics",
year = "2005",
doi = "10.1016/j.jmb.2005.08.076",
language = "English",
volume = "354",
pages = "16--24",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - Over Expression of a tRNALeu Isoacceptor Changes Charging Pattern of Leucine tRNAs and Reveals New Codon Reading

AU - Sørensen, Michael Askvad

AU - Elf, J.

AU - Bouakaz, E.

AU - Tenson, T.

AU - Sanyal, S.

AU - Björk, G. R.

AU - Ehrenberg, M.

N1 - Keywords: aminoacylation; systems biology; decoding; staruation; kinetics

PY - 2005

Y1 - 2005

N2 - During mRNA translation, synonymous codons for one amino acid areoften read by different isoaccepting tRNAs. The theory of selective tRNAcharging predicts greatly varying percentages of aminoacylation amongisoacceptors in cells starved for their common amino acid. It also predictsmajor changes in tRNA charging patterns upon concentration changes ofsingle isoacceptors, which suggests a novel type of translational control ofgene expression. We therefore tested the theory by measuring withNorthern blots the charging of Leu-tRNAs in Escherichia coli under Leulimitation in response to over expression of tRNALeuGAG. As predicted, thecharged level of tRNALeuGAG increased and the charged levels of four otherLeu isoacceptors decreased or remained unchanged, but the charged levelof tRNALeuUAG increased unexpectedly. To remove this apparent inconsistencybetween theory and experiment we postulated a previouslyunknown common codon for tRNALeuGAG and tRNALeuUAG. Subsequently, wedemonstrated that the tRNALeuGAG codon CUU is, in fact, read also bytRNALeuUAG, due to a uridine-5-oxyacetic acid modification.

AB - During mRNA translation, synonymous codons for one amino acid areoften read by different isoaccepting tRNAs. The theory of selective tRNAcharging predicts greatly varying percentages of aminoacylation amongisoacceptors in cells starved for their common amino acid. It also predictsmajor changes in tRNA charging patterns upon concentration changes ofsingle isoacceptors, which suggests a novel type of translational control ofgene expression. We therefore tested the theory by measuring withNorthern blots the charging of Leu-tRNAs in Escherichia coli under Leulimitation in response to over expression of tRNALeuGAG. As predicted, thecharged level of tRNALeuGAG increased and the charged levels of four otherLeu isoacceptors decreased or remained unchanged, but the charged levelof tRNALeuUAG increased unexpectedly. To remove this apparent inconsistencybetween theory and experiment we postulated a previouslyunknown common codon for tRNALeuGAG and tRNALeuUAG. Subsequently, wedemonstrated that the tRNALeuGAG codon CUU is, in fact, read also bytRNALeuUAG, due to a uridine-5-oxyacetic acid modification.

U2 - 10.1016/j.jmb.2005.08.076

DO - 10.1016/j.jmb.2005.08.076

M3 - Journal article

C2 - 16236318

VL - 354

SP - 16

EP - 24

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -

ID: 83580