Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone

Research output: Contribution to journal › Journal article › Research

Standard

Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone. / Nielsen, Olaf.

In: Cold Spring Harbor Protocols, Vol. 2016, No. 9, 2016, p. 768-772.

Research output: Contribution to journal › Journal article › Research

Harvard

Nielsen, O 2016, 'Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone', Cold Spring Harbor Protocols, vol. 2016, no. 9, pp. 768-772. https://doi.org/10.1101/pdb.prot091272

APA

Nielsen, O. (2016). Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone. Cold Spring Harbor Protocols, 2016(9), 768-772. https://doi.org/10.1101/pdb.prot091272

Vancouver

Nielsen O. Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone. Cold Spring Harbor Protocols. 2016;2016(9):768-772. https://doi.org/10.1101/pdb.prot091272

Author

Nielsen, Olaf. / Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone. In: Cold Spring Harbor Protocols. 2016 ; Vol. 2016, No. 9. pp. 768-772.

Bibtex

@article{a6b4b25a37264cebae533c24936c9e70,
title = "Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone",
abstract = "A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.",
author = "Olaf Nielsen",
year = "2016",
doi = "10.1101/pdb.prot091272",
language = "English",
volume = "2016",
pages = "768--772",
journal = "Cold Spring Harbor Protocols",
issn = "1940-3402",
publisher = "Cold Spring Harbor Laboratory Press",
number = "9",

}

RIS

TY - JOUR

T1 - Synchronization of S phase in Schizosaccharomyces pombe cells by transient exposure to M-factor pheromone

AU - Nielsen, Olaf

PY - 2016

Y1 - 2016

N2 - A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.

AB - A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.

U2 - 10.1101/pdb.prot091272

DO - 10.1101/pdb.prot091272

M3 - Journal article

C2 - 27587782

AN - SCOPUS:84985945892

VL - 2016

SP - 768

EP - 772

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 9

ER -

ID: 171657335