The LipB protein is a negative regulator of dam gene expression in Escherichia coli
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The LipB protein is a negative regulator of dam gene expression in Escherichia coli. / Vaisvila, Romas; Rasmussen, Lene Juel; Løbner-Olesen, Anders; von Freiesleben, Ulrik; Marinus, M. G.
In: Biochimica et Biophysica Acta, Gene Structure and Expression, Vol. 1494, No. 1-2, 2000, p. 43-53.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The LipB protein is a negative regulator of dam gene expression in Escherichia coli
AU - Vaisvila, Romas
AU - Rasmussen, Lene Juel
AU - Løbner-Olesen, Anders
AU - von Freiesleben, Ulrik
AU - Marinus, M. G.
PY - 2000
Y1 - 2000
N2 - Transcription initiation of the major promoter (P2) of the Escherichia coli dam gene increases with growth rate. The presence of three partially palindromic motifs, (TTCAGT(N(20))TGAG), designated G (growth)-boxes, within the -52 to +31 region of the promoter, may be related to growth rate control. Deletion of two of these repeats, downstream of the transcription initiation point, result in constitutive high activity of the promoter. The unlinked cde-4::miniTn10 insertion also results in severalfold higher activity of the dam P2 promoter, suggesting that this mutation resulted in the loss of a putative dam P2 repressor. The cde-4 mutation was mapped to the lipB (lipoic acid) gene, which we show encodes a 24 kDa protein initiating at a TTG codon. LipB is a highly conserved protein in animal and plant species, other bacteria, Archaea, and yeast. Plasmids expressing the native or hexahistidine-tagged LipB complement the phenotype of the cde-4 mutant strain. The level of LipB in vivo was higher in exponentially growing cells than those in the stationary phase. Three G-box motifs were also found in the lipB region. Models for the regulation of expression of the two genes are discussed.
AB - Transcription initiation of the major promoter (P2) of the Escherichia coli dam gene increases with growth rate. The presence of three partially palindromic motifs, (TTCAGT(N(20))TGAG), designated G (growth)-boxes, within the -52 to +31 region of the promoter, may be related to growth rate control. Deletion of two of these repeats, downstream of the transcription initiation point, result in constitutive high activity of the promoter. The unlinked cde-4::miniTn10 insertion also results in severalfold higher activity of the dam P2 promoter, suggesting that this mutation resulted in the loss of a putative dam P2 repressor. The cde-4 mutation was mapped to the lipB (lipoic acid) gene, which we show encodes a 24 kDa protein initiating at a TTG codon. LipB is a highly conserved protein in animal and plant species, other bacteria, Archaea, and yeast. Plasmids expressing the native or hexahistidine-tagged LipB complement the phenotype of the cde-4 mutant strain. The level of LipB in vivo was higher in exponentially growing cells than those in the stationary phase. Three G-box motifs were also found in the lipB region. Models for the regulation of expression of the two genes are discussed.
KW - Amino Acid Sequence
KW - Bacterial Proteins/chemistry
KW - Base Sequence
KW - Blotting, Western
KW - Cell Division
KW - Codon, Initiator/genetics
KW - Escherichia coli/genetics
KW - Gene Expression Regulation, Bacterial
KW - Genetic Complementation Test
KW - Molecular Sequence Data
KW - Mutation/genetics
KW - Operon/genetics
KW - Promoter Regions, Genetic/genetics
KW - Recombinant Fusion Proteins/isolation & purification
KW - Response Elements/genetics
KW - Sequence Alignment
U2 - 10.1016/S0167-4781(00)00209-8
DO - 10.1016/S0167-4781(00)00209-8
M3 - Journal article
C2 - 11072067
VL - 1494
SP - 43
EP - 53
JO - Biochimica et Biophysica Acta, Gene Structure and Expression
JF - Biochimica et Biophysica Acta, Gene Structure and Expression
SN - 0167-4781
IS - 1-2
ER -
ID: 200972423