ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes. / Hao, Qin; Hansen, Jacob B; Petersen, Rasmus K; Hallenborg, Philip; Jørgensen, Claus; Cinti, Saverio; Larsen, Philip J; Steffensen, Knut R; Wang, Haibo; Collins, Sheila; Wang, Jun; Gustafsson, Jan-Ake; Madsen, Lise; Kristiansen, Karsten.

In: BBA General Subjects, Vol. 1801, No. 4, 2010, p. 421-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hao, Q, Hansen, JB, Petersen, RK, Hallenborg, P, Jørgensen, C, Cinti, S, Larsen, PJ, Steffensen, KR, Wang, H, Collins, S, Wang, J, Gustafsson, J-A, Madsen, L & Kristiansen, K 2010, 'ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes', BBA General Subjects, vol. 1801, no. 4, pp. 421-9. https://doi.org/10.1016/j.bbalip.2009.11.008

APA

Hao, Q., Hansen, J. B., Petersen, R. K., Hallenborg, P., Jørgensen, C., Cinti, S., Larsen, P. J., Steffensen, K. R., Wang, H., Collins, S., Wang, J., Gustafsson, J-A., Madsen, L., & Kristiansen, K. (2010). ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes. BBA General Subjects, 1801(4), 421-9. https://doi.org/10.1016/j.bbalip.2009.11.008

Vancouver

Hao Q, Hansen JB, Petersen RK, Hallenborg P, Jørgensen C, Cinti S et al. ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes. BBA General Subjects. 2010;1801(4):421-9. https://doi.org/10.1016/j.bbalip.2009.11.008

Author

Hao, Qin ; Hansen, Jacob B ; Petersen, Rasmus K ; Hallenborg, Philip ; Jørgensen, Claus ; Cinti, Saverio ; Larsen, Philip J ; Steffensen, Knut R ; Wang, Haibo ; Collins, Sheila ; Wang, Jun ; Gustafsson, Jan-Ake ; Madsen, Lise ; Kristiansen, Karsten. / ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes. In: BBA General Subjects. 2010 ; Vol. 1801, No. 4. pp. 421-9.

Bibtex

@article{797b27a05c1f11df928f000ea68e967b,
title = "ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes",
abstract = "Cold adaptation elicits a paradoxical simultaneous induction of fatty acid synthesis and beta-oxidation in brown adipose tissue. We show here that cold exposure coordinately induced liver X receptor alpha (LXRalpha), adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC1alpha) in brown and inguinal white adipose tissues, but not in epididymal white adipose tissue. Using in vitro models of white and brown adipocytes we demonstrate that beta-adrenergic stimulation induced expression of LXRalpha, ADD1/SREBP1c and PGC1alpha in cells with a brown-like adipose phenotype. We demonstrate that ADD1/SREBP1c is a powerful inducer of PGC1alpha expression via a conserved E box in the proximal promoter and that beta-adrenergic stimulation led to recruitment of ADD1/SREBP1c to this E box. The ability of ADD1/SREBP1c to activate the PGC1alpha promoter exhibited a striking cell type dependency, suggesting that additional cell type-restricted factors contribute to ADD1/SREBP1c-mediated activation. In conclusion, our data demonstrate a novel role of ADD1/SREBP1c as a regulator of PGC1alpha expression in brown adipose tissue.",
author = "Qin Hao and Hansen, {Jacob B} and Petersen, {Rasmus K} and Philip Hallenborg and Claus J{\o}rgensen and Saverio Cinti and Larsen, {Philip J} and Steffensen, {Knut R} and Haibo Wang and Sheila Collins and Jun Wang and Jan-Ake Gustafsson and Lise Madsen and Karsten Kristiansen",
note = "Keywords: Adipocytes, Brown; Animals; Cell Differentiation; Cells, Cultured; Chromatin Immunoprecipitation; Electrophoretic Mobility Shift Assay; Electroporation; Male; Mice; Mice, Inbred C57BL; Orphan Nuclear Receptors; Promoter Regions, Genetic; RNA, Messenger; Regulatory Sequences, Nucleic Acid; Reverse Transcriptase Polymerase Chain Reaction; Sterol Regulatory Element Binding Protein 1; Trans-Activators",
year = "2010",
doi = "10.1016/j.bbalip.2009.11.008",
language = "English",
volume = "1801",
pages = "421--9",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - ADD1/SREBP1c activates the PGC1-alpha promoter in brown adipocytes

AU - Hao, Qin

AU - Hansen, Jacob B

AU - Petersen, Rasmus K

AU - Hallenborg, Philip

AU - Jørgensen, Claus

AU - Cinti, Saverio

AU - Larsen, Philip J

AU - Steffensen, Knut R

AU - Wang, Haibo

AU - Collins, Sheila

AU - Wang, Jun

AU - Gustafsson, Jan-Ake

AU - Madsen, Lise

AU - Kristiansen, Karsten

N1 - Keywords: Adipocytes, Brown; Animals; Cell Differentiation; Cells, Cultured; Chromatin Immunoprecipitation; Electrophoretic Mobility Shift Assay; Electroporation; Male; Mice; Mice, Inbred C57BL; Orphan Nuclear Receptors; Promoter Regions, Genetic; RNA, Messenger; Regulatory Sequences, Nucleic Acid; Reverse Transcriptase Polymerase Chain Reaction; Sterol Regulatory Element Binding Protein 1; Trans-Activators

PY - 2010

Y1 - 2010

N2 - Cold adaptation elicits a paradoxical simultaneous induction of fatty acid synthesis and beta-oxidation in brown adipose tissue. We show here that cold exposure coordinately induced liver X receptor alpha (LXRalpha), adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC1alpha) in brown and inguinal white adipose tissues, but not in epididymal white adipose tissue. Using in vitro models of white and brown adipocytes we demonstrate that beta-adrenergic stimulation induced expression of LXRalpha, ADD1/SREBP1c and PGC1alpha in cells with a brown-like adipose phenotype. We demonstrate that ADD1/SREBP1c is a powerful inducer of PGC1alpha expression via a conserved E box in the proximal promoter and that beta-adrenergic stimulation led to recruitment of ADD1/SREBP1c to this E box. The ability of ADD1/SREBP1c to activate the PGC1alpha promoter exhibited a striking cell type dependency, suggesting that additional cell type-restricted factors contribute to ADD1/SREBP1c-mediated activation. In conclusion, our data demonstrate a novel role of ADD1/SREBP1c as a regulator of PGC1alpha expression in brown adipose tissue.

AB - Cold adaptation elicits a paradoxical simultaneous induction of fatty acid synthesis and beta-oxidation in brown adipose tissue. We show here that cold exposure coordinately induced liver X receptor alpha (LXRalpha), adipocyte determination and differentiation-dependent factor 1 (ADD1)/sterol regulatory element-binding protein-1c (SREBP1c) and peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC1alpha) in brown and inguinal white adipose tissues, but not in epididymal white adipose tissue. Using in vitro models of white and brown adipocytes we demonstrate that beta-adrenergic stimulation induced expression of LXRalpha, ADD1/SREBP1c and PGC1alpha in cells with a brown-like adipose phenotype. We demonstrate that ADD1/SREBP1c is a powerful inducer of PGC1alpha expression via a conserved E box in the proximal promoter and that beta-adrenergic stimulation led to recruitment of ADD1/SREBP1c to this E box. The ability of ADD1/SREBP1c to activate the PGC1alpha promoter exhibited a striking cell type dependency, suggesting that additional cell type-restricted factors contribute to ADD1/SREBP1c-mediated activation. In conclusion, our data demonstrate a novel role of ADD1/SREBP1c as a regulator of PGC1alpha expression in brown adipose tissue.

U2 - 10.1016/j.bbalip.2009.11.008

DO - 10.1016/j.bbalip.2009.11.008

M3 - Journal article

C2 - 19962449

VL - 1801

SP - 421

EP - 429

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 4

ER -

ID: 19664243