Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis
Research output: Contribution to journal › Journal article › Research › peer-review
The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
Original language | English |
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Journal | European Journal of Biochemistry |
Volume | 83 |
Issue number | 2 |
Pages (from-to) | 389-94 |
Number of pages | 5 |
ISSN | 0014-2956 |
Publication status | Published - 1978 |
Externally published | Yes |
Bibliographical note
Keywords: Acetone; Animals; Cell Fractionation; Molecular Weight; Protein Biosynthesis; Ribosomal Proteins; Ribosomes; Tetrahymena pyriformis
ID: 11368600