Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development. / Kiilerich, Pia; Pedersen, Sara H.; Kristiansen, Karsten; Madsen, Steffen S.

In: General and Comparative Endocrinology, Vol. 170, No. 2, 2011, p. 283-289.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kiilerich, P, Pedersen, SH, Kristiansen, K & Madsen, SS 2011, 'Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development', General and Comparative Endocrinology, vol. 170, no. 2, pp. 283-289. https://doi.org/10.1016/j.ygcen.2010.02.014

APA

Kiilerich, P., Pedersen, S. H., Kristiansen, K., & Madsen, S. S. (2011). Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development. General and Comparative Endocrinology, 170(2), 283-289. https://doi.org/10.1016/j.ygcen.2010.02.014

Vancouver

Kiilerich P, Pedersen SH, Kristiansen K, Madsen SS. Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development. General and Comparative Endocrinology. 2011;170(2):283-289. https://doi.org/10.1016/j.ygcen.2010.02.014

Author

Kiilerich, Pia ; Pedersen, Sara H. ; Kristiansen, Karsten ; Madsen, Steffen S. / Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development. In: General and Comparative Endocrinology. 2011 ; Vol. 170, No. 2. pp. 283-289.

Bibtex

@article{85ad89b0a8f911df928f000ea68e967b,
title = "Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development",
abstract = "The proposed mineralocorticoid-like signalling axis in teleost fish, consisting of the hormone 11-deoxycorticosterone (DOC) and a mineralocorticoid receptor (MR), has recently challenged our conception of cortisol being the only osmoregulatory corticosteroid in teleost fish. This paper aimed at comparing the osmoregulatory role of DOC with that of cortisol during the pre-adaptive development of SW-tolerance, smoltification, in Atlantic salmon. Using an in vitro gill block incubation system, the effect of DOC and cortisol in the gill was investigated from January to September, using Na(+),K(+)-ATPase alpha-subunit isoforms alpha-1a and alpha-1b mRNA levels as targets for regulation by the hormones. Cortisol and DOC both conferred significant up-regulation of alpha-1a and alpha-1b mRNA levels at specific time-points during smoltification. However, the effect of cortisol and DOC on alpha-subunit isoforms varied seasonally between isoforms and hormones. The maximum induction of alpha-1a was 3- to 4-fold compared to controls whereas a 2-fold induction was observed for alpha-1b. The pattern and capacity of stimulation of alpha-1a through smoltification were similar for cortisol and DOC, whereas cortisol had an enhanced capacity to stimulate alpha-1b as compared to DOC. Even though there was no demonstrable change in cortisol or DOC sensitivity in the gill, the magnitude of the hormonal effects were seasonally dependent. This is the first report of DOC-induced effects on osmoregulatory targets in fish, thus indicating a role for DOC and MR signalling in osmoregulation.",
author = "Pia Kiilerich and Pedersen, {Sara H.} and Karsten Kristiansen and Madsen, {Steffen S.}",
note = "Keywords: 11-Deoxycorticosterone; DOC; Cortisol; In vitro gill block incubation; Na+,K+-ATPase isoforms; Na+,K+-ATPase a1a; Na+,K+-ATPase a1b; Atlantic salmon; Gill; Smoltification; Glucocorticoid receptor; GR1; GR2; Mineralocorticoid receptor; M",
year = "2011",
doi = "10.1016/j.ygcen.2010.02.014",
language = "English",
volume = "170",
pages = "283--289",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Corticosteroid regulation of Na+,K+-ATPase α1-isoform expression in Atlantic salmon gill during smolt development

AU - Kiilerich, Pia

AU - Pedersen, Sara H.

AU - Kristiansen, Karsten

AU - Madsen, Steffen S.

N1 - Keywords: 11-Deoxycorticosterone; DOC; Cortisol; In vitro gill block incubation; Na+,K+-ATPase isoforms; Na+,K+-ATPase a1a; Na+,K+-ATPase a1b; Atlantic salmon; Gill; Smoltification; Glucocorticoid receptor; GR1; GR2; Mineralocorticoid receptor; M

PY - 2011

Y1 - 2011

N2 - The proposed mineralocorticoid-like signalling axis in teleost fish, consisting of the hormone 11-deoxycorticosterone (DOC) and a mineralocorticoid receptor (MR), has recently challenged our conception of cortisol being the only osmoregulatory corticosteroid in teleost fish. This paper aimed at comparing the osmoregulatory role of DOC with that of cortisol during the pre-adaptive development of SW-tolerance, smoltification, in Atlantic salmon. Using an in vitro gill block incubation system, the effect of DOC and cortisol in the gill was investigated from January to September, using Na(+),K(+)-ATPase alpha-subunit isoforms alpha-1a and alpha-1b mRNA levels as targets for regulation by the hormones. Cortisol and DOC both conferred significant up-regulation of alpha-1a and alpha-1b mRNA levels at specific time-points during smoltification. However, the effect of cortisol and DOC on alpha-subunit isoforms varied seasonally between isoforms and hormones. The maximum induction of alpha-1a was 3- to 4-fold compared to controls whereas a 2-fold induction was observed for alpha-1b. The pattern and capacity of stimulation of alpha-1a through smoltification were similar for cortisol and DOC, whereas cortisol had an enhanced capacity to stimulate alpha-1b as compared to DOC. Even though there was no demonstrable change in cortisol or DOC sensitivity in the gill, the magnitude of the hormonal effects were seasonally dependent. This is the first report of DOC-induced effects on osmoregulatory targets in fish, thus indicating a role for DOC and MR signalling in osmoregulation.

AB - The proposed mineralocorticoid-like signalling axis in teleost fish, consisting of the hormone 11-deoxycorticosterone (DOC) and a mineralocorticoid receptor (MR), has recently challenged our conception of cortisol being the only osmoregulatory corticosteroid in teleost fish. This paper aimed at comparing the osmoregulatory role of DOC with that of cortisol during the pre-adaptive development of SW-tolerance, smoltification, in Atlantic salmon. Using an in vitro gill block incubation system, the effect of DOC and cortisol in the gill was investigated from January to September, using Na(+),K(+)-ATPase alpha-subunit isoforms alpha-1a and alpha-1b mRNA levels as targets for regulation by the hormones. Cortisol and DOC both conferred significant up-regulation of alpha-1a and alpha-1b mRNA levels at specific time-points during smoltification. However, the effect of cortisol and DOC on alpha-subunit isoforms varied seasonally between isoforms and hormones. The maximum induction of alpha-1a was 3- to 4-fold compared to controls whereas a 2-fold induction was observed for alpha-1b. The pattern and capacity of stimulation of alpha-1a through smoltification were similar for cortisol and DOC, whereas cortisol had an enhanced capacity to stimulate alpha-1b as compared to DOC. Even though there was no demonstrable change in cortisol or DOC sensitivity in the gill, the magnitude of the hormonal effects were seasonally dependent. This is the first report of DOC-induced effects on osmoregulatory targets in fish, thus indicating a role for DOC and MR signalling in osmoregulation.

U2 - 10.1016/j.ygcen.2010.02.014

DO - 10.1016/j.ygcen.2010.02.014

M3 - Journal article

C2 - 20171217

VL - 170

SP - 283

EP - 289

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 2

ER -

ID: 21404977