Expression of recombinant proteins: an introduction
Research output: Contribution to journal › Journal article › Research › peer-review
To better understand and characterize the function (or lack thereof) of proteins-wild-type vs mutants-it is advantageous to be able to express these proteins in a controlled, chosen setup. For downstream applications such as enzymatic assays, X-ray crystallography/nuclear magnetic resonance (NMR), raising antibodies, or characterization of physical interaction with other proteins, it may furthermore be desirable to obtain large quantities of pure protein.
Original language | English |
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Journal | Methods in Molecular Biology |
Volume | 232 |
Pages (from-to) | 93-101 |
Number of pages | 8 |
ISSN | 1064-3745 |
DOIs | |
Publication status | Published - 2003 |
Externally published | Yes |
Bibliographical note
Keywords: Animals; Biotechnology; Protein Engineering; Recombinant Proteins
ID: 11230768