Genetic and metabolic diversity of arctic bacterioplankton during the post-spring phytoplankton bloom in Disko Bay, western Greenland
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Genetic and metabolic diversity of arctic bacterioplankton during the post-spring phytoplankton bloom in Disko Bay, western Greenland. / Frette, Lone; Winding, Anne; Kroer, Niels.
In: Aquatic Microbial Ecology, Vol. 60, No. 1, 04.05.2010, p. 29-41.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Genetic and metabolic diversity of arctic bacterioplankton during the post-spring phytoplankton bloom in Disko Bay, western Greenland
AU - Frette, Lone
AU - Winding, Anne
AU - Kroer, Niels
PY - 2010/5/4
Y1 - 2010/5/4
N2 - Changes in the genetic and metabolic diversity of the bacterioplankton in Disko Bay, western Greenland, were followed during the post-spring phytoplankton bloom. Samples were taken at different depths every 2 to 3 d from 1 to 23 June 2001. The highest bacterial abundance was found at the depth of the fluorescence maximum (FM). Average cell numbers at FM were around 6 x 105 cells ml-1, but numbers were highly variable. Immediately following a rapid decline in the phytoplankton biomass from 31 to 8 mg chl a m-3, a peak in numbers of culturable bacteria was observed reaching 3 x 104 CFU ml-1. Concomitant with the changes in phytoplankton biomass and bacterial culturability, the genetic diversity of the bacterioplankton communities changed as assessed by DGGE. At FM, DGGE fingerprints shifted between 6 and 8 June; however, they returned to the initial profile between 10 and 14 June. Similarly, successional shifts in the diversity of the culturable bacteria were observed as the populations before and after the peak in CFUs were significantly different. Accompanying the changes in genetic diversity, the metabolic diversity of the bacterial communities shifted and the communities became adapted to growth on refractory carbon sources such as cellulose and xylan. Our investigation thus demonstrates intimate links between phytoplankton and bacterioplankton and that the spring phytoplankton bloom strongly affects the genetic diversity and functional characteristics of the heterotrophic bacterial communities in Disko Bay.
AB - Changes in the genetic and metabolic diversity of the bacterioplankton in Disko Bay, western Greenland, were followed during the post-spring phytoplankton bloom. Samples were taken at different depths every 2 to 3 d from 1 to 23 June 2001. The highest bacterial abundance was found at the depth of the fluorescence maximum (FM). Average cell numbers at FM were around 6 x 105 cells ml-1, but numbers were highly variable. Immediately following a rapid decline in the phytoplankton biomass from 31 to 8 mg chl a m-3, a peak in numbers of culturable bacteria was observed reaching 3 x 104 CFU ml-1. Concomitant with the changes in phytoplankton biomass and bacterial culturability, the genetic diversity of the bacterioplankton communities changed as assessed by DGGE. At FM, DGGE fingerprints shifted between 6 and 8 June; however, they returned to the initial profile between 10 and 14 June. Similarly, successional shifts in the diversity of the culturable bacteria were observed as the populations before and after the peak in CFUs were significantly different. Accompanying the changes in genetic diversity, the metabolic diversity of the bacterial communities shifted and the communities became adapted to growth on refractory carbon sources such as cellulose and xylan. Our investigation thus demonstrates intimate links between phytoplankton and bacterioplankton and that the spring phytoplankton bloom strongly affects the genetic diversity and functional characteristics of the heterotrophic bacterial communities in Disko Bay.
KW - Arctic
KW - Bacterioplankton
KW - Genetic diversity
KW - Metabolic diversity
KW - Spring bloom
UR - http://www.scopus.com/inward/record.url?scp=77954291932&partnerID=8YFLogxK
U2 - 10.3354/ame01410
DO - 10.3354/ame01410
M3 - Journal article
AN - SCOPUS:77954291932
VL - 60
SP - 29
EP - 41
JO - Aquatic Microbial Ecology
JF - Aquatic Microbial Ecology
SN - 0948-3055
IS - 1
ER -
ID: 214687339