Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon

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Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon. / Jaubert, Carole; Danioux, Chloë; Oberto, Jacques; Cortez, Diego; Bize, Ariane; Krupovic, Mart; She, Qunxin; Forterre, Patrick; Prangishvili, David; Sezonov, Guennadi.

In: Open Biology, Vol. 3, No. 4, 2013.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jaubert, C, Danioux, C, Oberto, J, Cortez, D, Bize, A, Krupovic, M, She, Q, Forterre, P, Prangishvili, D & Sezonov, G 2013, 'Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon', Open Biology, vol. 3, no. 4. https://doi.org/10.1098/rsob.130010

APA

Jaubert, C., Danioux, C., Oberto, J., Cortez, D., Bize, A., Krupovic, M., She, Q., Forterre, P., Prangishvili, D., & Sezonov, G. (2013). Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon. Open Biology, 3(4). https://doi.org/10.1098/rsob.130010

Vancouver

Jaubert C, Danioux C, Oberto J, Cortez D, Bize A, Krupovic M et al. Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon. Open Biology. 2013;3(4). https://doi.org/10.1098/rsob.130010

Author

Jaubert, Carole ; Danioux, Chloë ; Oberto, Jacques ; Cortez, Diego ; Bize, Ariane ; Krupovic, Mart ; She, Qunxin ; Forterre, Patrick ; Prangishvili, David ; Sezonov, Guennadi. / Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon. In: Open Biology. 2013 ; Vol. 3, No. 4.

Bibtex

@article{0e35d09921c24b459b5c77915ce827d5,
title = "Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon",
abstract = "The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S. islandicus strains isolated from Iceland, Russia and USA revealed a highly syntenic common core genome of approximately 2 Mb and a long hyperplastic region containing most of the strain-specific genes. In LAL14/1, the latter region is enriched in insertion sequences, CRISPR (clustered regularly interspaced short palindromic repeats), glycosyl transferase genes, toxin-antitoxin genes and MITE (miniature inverted-repeat transposable elements). The tRNA genes of LAL14/1 are preferential targets for the integration of mobile elements but clusters of atypical genes (CAG) are also integrated elsewhere in the genome. LAL14/1 carries five CRISPR loci with 10 per cent of spacers matching perfectly or imperfectly the genomes of archaeal viruses and plasmids found in the Icelandic hot springs. Strikingly, the CRISPR_2 region of LAL14/1 carries an unusually long 1.9 kb spacer interspersed between two repeat regions and displays a high similarity to pING1-like conjugative plasmids. Finally, we have developed a genetic system for S. islandicus LAL14/1 and created ¿pyrEF and ¿CRISPR_1 mutants using double cross-over and pop-in/pop-out approaches, respectively. Thus, LAL14/1 is a promising model to study virus-host interactions and the CRISPR/Cas defence mechanism in Archaea.",
author = "Carole Jaubert and Chlo{\"e} Danioux and Jacques Oberto and Diego Cortez and Ariane Bize and Mart Krupovic and Qunxin She and Patrick Forterre and David Prangishvili and Guennadi Sezonov",
year = "2013",
doi = "10.1098/rsob.130010",
language = "English",
volume = "3",
journal = "Open Biology",
issn = "2046-2441",
publisher = "TheRoyal Society Publishing",
number = "4",

}

RIS

TY - JOUR

T1 - Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon

AU - Jaubert, Carole

AU - Danioux, Chloë

AU - Oberto, Jacques

AU - Cortez, Diego

AU - Bize, Ariane

AU - Krupovic, Mart

AU - She, Qunxin

AU - Forterre, Patrick

AU - Prangishvili, David

AU - Sezonov, Guennadi

PY - 2013

Y1 - 2013

N2 - The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S. islandicus strains isolated from Iceland, Russia and USA revealed a highly syntenic common core genome of approximately 2 Mb and a long hyperplastic region containing most of the strain-specific genes. In LAL14/1, the latter region is enriched in insertion sequences, CRISPR (clustered regularly interspaced short palindromic repeats), glycosyl transferase genes, toxin-antitoxin genes and MITE (miniature inverted-repeat transposable elements). The tRNA genes of LAL14/1 are preferential targets for the integration of mobile elements but clusters of atypical genes (CAG) are also integrated elsewhere in the genome. LAL14/1 carries five CRISPR loci with 10 per cent of spacers matching perfectly or imperfectly the genomes of archaeal viruses and plasmids found in the Icelandic hot springs. Strikingly, the CRISPR_2 region of LAL14/1 carries an unusually long 1.9 kb spacer interspersed between two repeat regions and displays a high similarity to pING1-like conjugative plasmids. Finally, we have developed a genetic system for S. islandicus LAL14/1 and created ¿pyrEF and ¿CRISPR_1 mutants using double cross-over and pop-in/pop-out approaches, respectively. Thus, LAL14/1 is a promising model to study virus-host interactions and the CRISPR/Cas defence mechanism in Archaea.

AB - The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S. islandicus strains isolated from Iceland, Russia and USA revealed a highly syntenic common core genome of approximately 2 Mb and a long hyperplastic region containing most of the strain-specific genes. In LAL14/1, the latter region is enriched in insertion sequences, CRISPR (clustered regularly interspaced short palindromic repeats), glycosyl transferase genes, toxin-antitoxin genes and MITE (miniature inverted-repeat transposable elements). The tRNA genes of LAL14/1 are preferential targets for the integration of mobile elements but clusters of atypical genes (CAG) are also integrated elsewhere in the genome. LAL14/1 carries five CRISPR loci with 10 per cent of spacers matching perfectly or imperfectly the genomes of archaeal viruses and plasmids found in the Icelandic hot springs. Strikingly, the CRISPR_2 region of LAL14/1 carries an unusually long 1.9 kb spacer interspersed between two repeat regions and displays a high similarity to pING1-like conjugative plasmids. Finally, we have developed a genetic system for S. islandicus LAL14/1 and created ¿pyrEF and ¿CRISPR_1 mutants using double cross-over and pop-in/pop-out approaches, respectively. Thus, LAL14/1 is a promising model to study virus-host interactions and the CRISPR/Cas defence mechanism in Archaea.

U2 - 10.1098/rsob.130010

DO - 10.1098/rsob.130010

M3 - Journal article

C2 - 23594878

VL - 3

JO - Open Biology

JF - Open Biology

SN - 2046-2441

IS - 4

ER -

ID: 45218614