Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis
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Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis. / Christensen, P U; Bentley, N J; Martinho, R G; Nielsen, O; Carr, Antony; Nielsen, Olaf.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 6, 14.03.2000, p. 2579-84.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis
AU - Christensen, P U
AU - Bentley, N J
AU - Martinho, R G
AU - Nielsen, O
AU - Carr, Antony
AU - Nielsen, Olaf
PY - 2000/3/14
Y1 - 2000/3/14
N2 - Two paradigms exist for maintaining order during cell-cycle progression: intrinsic controls, where passage through one part of the cell cycle directly affects the ability to execute another, and checkpoint controls, where external pathways impose order in response to aberrant structures. By studying the mitotic inhibitor Mik1, we have identified evidence for an intrinsic link between unperturbed S phase and mitosis. We propose a model in which S/M linkage can be generated by the production and stabilization of Mik1 protein during S phase. The production of Mik1 during unperturbed S phase is independent of the Rad3- and Cds1-dependent checkpoint controls. In response to perturbed S phase, Rad3-Cds1 checkpoint controls are required to maintain high levels of Mik1, probably indirectly by extending the S phase period, where Mik1 is stable. In addition, we find that Mik1 protein can be moderately induced in response to irradiation of G(2) cells in a Chk1-dependent manner.
AB - Two paradigms exist for maintaining order during cell-cycle progression: intrinsic controls, where passage through one part of the cell cycle directly affects the ability to execute another, and checkpoint controls, where external pathways impose order in response to aberrant structures. By studying the mitotic inhibitor Mik1, we have identified evidence for an intrinsic link between unperturbed S phase and mitosis. We propose a model in which S/M linkage can be generated by the production and stabilization of Mik1 protein during S phase. The production of Mik1 during unperturbed S phase is independent of the Rad3- and Cds1-dependent checkpoint controls. In response to perturbed S phase, Rad3-Cds1 checkpoint controls are required to maintain high levels of Mik1, probably indirectly by extending the S phase period, where Mik1 is stable. In addition, we find that Mik1 protein can be moderately induced in response to irradiation of G(2) cells in a Chk1-dependent manner.
KW - Enzyme Inhibitors
KW - Epitopes
KW - Flow Cytometry
KW - G2 Phase
KW - Hydroxyurea
KW - Immunoblotting
KW - Microscopy, Fluorescence
KW - Mitosis
KW - Models, Biological
KW - Phosphoric Monoester Hydrolases
KW - Phosphorylation
KW - Protein Kinases
KW - Protein-Tyrosine Kinases
KW - S Phase
KW - Schizosaccharomyces
KW - Schizosaccharomyces pombe Proteins
KW - Time Factors
M3 - Journal article
C2 - 10716991
VL - 97
SP - 2579
EP - 2584
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 6
ER -
ID: 33576861