Quantitation of Dam methyltransferase in Escherichia coli
Research output: Contribution to journal › Journal article › Research › peer-review
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.
| Original language | English |
|---|---|
| Journal | Journal of Bacteriology |
| Volume | 174 |
| Issue number | 5 |
| Pages (from-to) | 1682-1685 |
| Number of pages | 4 |
| ISSN | 0021-9193 |
| DOIs | |
| Publication status | Published - 1992 |
| Externally published | Yes |
- Blotting, Western, Escherichia coli/enzymology, Escherichia coli Proteins, Immunoenzyme Techniques, Methyltransferases/analysis, Site-Specific DNA-Methyltransferase (Adenine-Specific)
Research areas
ID: 200973014