Jakob R. Winther

Jakob R. Winther

Professor

ORCID: 0000-0001-6995-9154

Publication year:
All
1 - 113 out of 113Page size: 500
  1. 2023
  2. Published

    Global analysis of multi-mutants to improve protein function

    Johansson, Kristoffer Enøe, Lindorff-Larsen, Kresten & Winther, Jakob R., 2023, In: Journal of Molecular Biology. 435, 8, 15 p., 168034.

    Research output: Contribution to journal › Journal article › Research › peer-review

  3. 2022
  4. Published

    Co-evolution of drug resistance and broadened substrate recognition in HIV protease variants isolated from an Escherichia coli genetic selection system

    Koivisto, J. M., Poulsen, N. R., Larsen, B. S., Weibull, M. G. M., Stein, Amelie, Doro, F., Winther, Jakob R., Lindorff-Larsen, Kresten & Willemoës, Martin, 2022, In: The Biochemical journal. 479, 4, p. 479-501

    Research output: Contribution to journal › Journal article › Research › peer-review

  5. Published

    Engineering Bifidobacterium longum Endo-α-N-acetylgalactosaminidase for Neu5Acα2-3Ga1β1-3Ga1NAc reactivity on Fetuin

    Hansen, D. K., Hansen, A. L., Koivisto, J. M., Shuoker, B., Abou Hachem, M., Winther, Jakob R. & Willemoës, Martin, 2022, In: Archives of Biochemistry and Biophysics. 725, 8 p., 109280.

    Research output: Contribution to journal › Journal article › Research › peer-review

  6. Published

    Increasing protein stability by inferring substitution effects from high-throughput experiments

    Norrild, R. K., Johansson, Kristoffer Enøe, O'Shea, Charlotte, Morth, J. P., Lindorff-Larsen, Kresten & Winther, Jakob R., 2022, In: Cell Reports Methods. 2, 11, 11 p., 100333.

    Research output: Contribution to journal › Journal article › Research › peer-review

  7. Published

    The functional role of the autolysis loop in the regulation of factor X upon hemostatic response

    Bonde, A. C., Lund, J., Hansen, J. J., Winther, Jakob R., Nielsen, P. F., Zahn, S., Tiainen, P., Olsen, O. H., Petersen, H. H. & Rose Bjelke, J., 2022, In: Journal of Thrombosis and Haemostasis. 20, 3, p. 589-599 11 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  8. 2021
  9. Published

    VM i molekylære puslespil

    Abildgaard, A. B., Winther, Jakob R., Lindorff-Larsen, Kresten & Hartmann-Petersen, Rasmus, 16 Apr 2021, In: Weekendavisen: Ideer. 15, p. 11

    Research output: Contribution to journal › Contribution to newspaper - Feature article › Communication

  10. Published

    Molekylære stavefejl

    Abildgaard, A. B., Winther, Jakob R., Lindorff-Larsen, Kresten & Hartmann-Petersen, Rasmus, 28 Jan 2021, In: Weekendavisen, Ideer.

    Research output: Contribution to journal › Contribution to newspaper - Feature article › Communication

  11. Published

    Charge Engineering Reveals the Roles of Ionizable Side Chains in Electrospray Ionization Mass Spectrometry

    Abramsson, M. L., Sahin, C., Hopper, J. T. S., Branca, R. M. M., Danielsson, J., Xu, M., Chandler, S. A., Österlund, N., Ilag, L. L., Leppert, A., Costeira-Paulo, J., Lang, L., Teilum, K., Laganowsky, A., Benesch, J. L. P., Oliveberg, M., Robinson, C. V., Marklund, E. G., Allison, T. M., Winther, J. R. & 1 others, Landreh, M., 2021, In: JACS Au. 1, 12, p. 2385-2393 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  12. Published

    Charge Interactions in a Highly Charge-Depleted Protein

    Hervø-Hansen, S., Højgaard, C., Johansson, Kristoffer Enøe, Wang, Yong, Wahni, K., Young, D., Messens, J., Teilum, Kaare, Lindorff-Larsen, Kresten & Winther, Jakob R., 2021, In: Journal of the American Chemical Society. 143, 6, p. 2500-2508 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  13. Published

    Computational and Experimental Assessment of Backbone Templates for Computational Redesign of the Thioredoxin Fold

    Marin, Frederikke Isa, Johansson, Kristoffer Enøe, O'Shea, Charlotte, Lindorff-Larsen, Kresten & Winther, Jakob R., 2021, In: The Journal of Physical Chemistry B. 125, 40, p. 11141-11149

    Research output: Contribution to journal › Journal article › Research › peer-review

  14. Published

    Computational and experimental assessment of backbone templates for computational protein design

    Marin, Frederikke Isa, Johansson, K. E., O'Shea, Charlotte, Lindorff-Larsen, Kresten & Winther, Jakob R., 2021, 15 p.

    Research output: Working paper › Preprint › Research

  15. Published

    Flash properties of Gaussia luciferase are the result of covalent inhibition after a limited number of cycles

    Dijkema, F. M., Nordentoft, M. K., Didriksen, A. K., Corneliussen, A. S., Willemoës, Martin & Winther, Jakob R., 2021, In: Protein Science. 30, 3, p. 638-649 12 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  16. Published

    Rational Protein Engineering to Increase the Activity and Stability of IsPETase Using the PROSS Algorithm

    Rennison, A., Winther, Jakob R. & Varrone, C., 2021, In: Polymers. 13, 22, 11 p., 3884.

    Research output: Contribution to journal › Journal article › Research › peer-review

  17. Published

    Substitutional landscape of a split fluorescent protein fragment using high-density peptide microarrays

    Antonescu, O. N., Rasmussen, A., Damm, N. A. M., Heidemann, Ditte Fogh, Popov, R., Nesterov-Mueller, A., Johansson, Kristoffer Enøe & Winther, Jakob R., 2021, In: PLoS ONE. 16, 2, 17 p., e0241461.

    Research output: Contribution to journal › Journal article › Research › peer-review

  18. 2020
  19. Published

    Mutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water

    Kampmeyer, Caroline, Johansen, Jens Vilstrup, Holmberg, Christian, Karlson, M., Andersen, Sarah Gersing, Nunes Bordallo, Heloisa, Kragelund, Birthe Brandt, Lerche, M. H., Jourdain, I., Winther, Jakob R. & Hartmann-Petersen, Rasmus, 2020, In: A C S Synthetic Biology. 9, 4, p. 733-748 16 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  20. 2019
  21. Published

    Improving folding properties of computationally designed proteins

    Bjerre, B., Nissen, J., Madsen, M., Kamarauskaite, J., Norrild, R. K., Holm, Peter Christoffer, Nordentoft, M. K., O'Shea, Charlotte, Willemoës, Martin, Johansson, Kristoffer Enøe & Winther, Jakob R., 13 Dec 2019, In: Protein Engineering Design and Selection (Print). 32, 3, p. 145-151 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  22. Published

    Shining light on split fluorescent protein fitness landscapes using peptide array-based deep substitutional scanning

    ANTONESCU, O., Johansson, Kristoffer Enøe & Winther, Jakob R., 30 Jun 2019.

    Research output: Contribution to conference › Poster › Research › peer-review

  23. Published

    Pulmonary effects of nanofibrillated celluloses in mice suggest that carboxylation lowers the inflammatory and acute phase responses

    Hadrup, N., Knudsen, K. B., Berthing, T., Wolff, H., Bengtson, S., Kofoed, Christian, Espersen, R., Højgaard, C., Winther, Jakob R., Willemoës, Martin, Wedin, I., Nuopponen, M., Alenius, H., Norppa, H., Wallin, H. & Vogel, U., 2019, In: Environmental Toxicology and Pharmacology. 66, p. 116-125 10 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  24. Published

    Steady state kinetic analysis of O-linked GalNAc glycan release catalyzed by endo-α-N-acetylgalactosaminidase

    Hansen, D. K., Winther, Jakob R. & Willemoës, Martin, 2019, In: Carbohydrate Research. 480, p. 54-60

    Research output: Contribution to journal › Journal article › Research › peer-review

  25. 2018
  26. Published

    Comprehensive mapping of GFP structure-function relationship using high-density peptide arrays

    Antonescu, O., Johansson, Kristoffer Enøe & Winther, Jakob R., 10 Jul 2018.

    Research output: Contribution to conference › Poster › Research › peer-review

  27. Published

    A Robust Proton Flux (pHlux) Assay for Studying the Function and Inhibition of the Influenza A M2 Proton Channel

    Santner, P., Martins, J. M. D. S., Laursen, J. S., Behrendt, L., Riber, Leise, Olsen, Christian Adam, Arkin, I. T., Winther, Jakob R., Willemoës, Martin & Lindorff-Larsen, Kresten, 2018, In: Biochemistry. 57, 41, p. 5949-5956

    Research output: Contribution to journal › Journal article › Research › peer-review

  28. Published

    Can a Charged Surfactant Unfold an Uncharged Protein?

    Højgaard, C., Sørensen, H. V., Pedersen, J. S., Winther, Jakob R. & Otzen, D. E., 2018, In: Biophysical Journal. 115, 11, p. 2081-2086 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  29. Published

    Editorial overview: A perspective on protein evolution

    Hoecker, B. & Winther, Jakob R., 2018, In: Current Opinion in Structural Biology. 48, p. viii-ix 2 p.

    Research output: Contribution to journal › Editorial › Research › peer-review

  30. Published

    Nanofibrillated cellulose causes acute pulmonary inflammation that subsides within a month

    Ilves, M., Vilske, S., Aimonen, K., Lindberg, H. K., Pesonen, S., Wedin, I., Nuopponen, M., Vanhala, E., Højgaard, C., Winther, Jakob R., Willemoës, Martin, Vogel, U., Wolff, H., Norppa, H., Savolainen, K. & Alenius, H., 2018, In: Nanotoxicology. 12, 7, p. 729-746 18 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  31. Published

    Protein evolution: Hacking an enzyme

    Johansson, Kristoffer Enøe & Winther, Jakob R., 2018, In: Nature Chemical Biology. 14, 3, p. 202-204 3 p.

    Research output: Contribution to journal › Comment/debate › Research › peer-review

  32. Published

    Random mutagenesis analysis of the Influenza A M2 proton channel reveals novel resistance mutants

    Santner, P., Martins, J. M. D. S., Kampmeyer, Caroline, Hartmann-Petersen, Rasmus, Laursen, J. S., Stein, Amelie, Olsen, Christian Adam, Arkin, I. T., Winther, Jakob R., Willemoës, Martin & Lindorff-Larsen, Kresten, 2018, In: Biochemistry. 57, 41, p. 5957-5968

    Research output: Contribution to journal › Journal article › Research › peer-review

  33. 2017
  34. Published

    Evolution i overhalingsbanen

    Abildgaard, A. B., Hartmann-Petersen, Rasmus, Lindorff-Larsen, Kresten & Winther, Jakob R., 15 Dec 2017, In: Weekendavisen. 2017, 50, p. Ideer: 13 1 p.

    Research output: Contribution to journal › Contribution to newspaper - Feature article › Communication

  35. Published

    Endoplasmic reticulum transport of glutathione by Sec61 is regulated by Ero1 and Bip

    Ponsero, A. J., Igbaria, A., Darch, M. A., Miled, S., Outten, C. E., Winther, Jakob R., Palais, G., D'Autréaux, B., Delaunay-Moisan, A. & Toledano, M. B., 21 Sep 2017, In: Molecular Cell. 67, 6, p. 962-973.e5 18 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  36. Published

    Positive staining for cellulose in oral pulse granuloma

    Virkkunen, S., Wolff, H., Haglund, C., Højgaard, C., Winther, Jakob R., Willemoës, Martin, Vogel, U. & Hagström, J., Apr 2017, In: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 123, 4, p. 464-467 4 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  37. Published

    Exploring the antigenic response to multiplexed immunizations in a chicken model of antibody production

    Kousted, T. M., Kalliokoski, Otto, Christensen, S. K., Winther, Jakob R. & Hau, Jann, Mar 2017, In: Heliyon. 3, 3, 19 p., e00267.

    Research output: Contribution to journal › Journal article › Research › peer-review

  38. 2016
  39. Published

    A soluble, folded protein without charged amino acid residues

    Højgaard, C., Kofoed, Christian, Espersen, R., Johansson, Kristoffer Enøe, Villa, M., Willemoës, Martin, Lindorff-Larsen, Kresten, Teilum, Kaare & Winther, Jakob R., 2016, In: Biochemistry. 55, 28, p. 3949–3956 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  40. Published

    Computational redesign of thioredoxin is hypersensitive towards minor conformational changes in the backbone template

    Johansson, Kristoffer Enøe, Johansen, Nicolai Tidemand, Christensen, S., Horowitz, S., Bardwell, J. C. A., Olsen, Johan Gotthardt, Willemoës, Martin, Lindorff-Larsen, Kresten, Ferkinghoff-Borg, J., Hamelryck, Thomas Wim & Winther, Jakob R., 2016, In: Journal of Molecular Biology. 428, 21, p. 4361-4377 17 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  41. 2015
  42. Published

    Mutational analysis of divalent metal ion binding in the active site of class II α-mannosidase from Sulfolobus solfataricus

    Hansen, D., Webb, H., Nielsen, J. W., Harris, P., Winther, Jakob R. & Willemoës, Martin, 2015, In: Biochemistry. 54, 11, p. 2032-2039 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  43. Published

    Visualization of nanofibrillar cellulose in biological tissues using a biotinylated carbohydrate binding module of β-1,4-Glycanase

    Knudsen, K. B., Kofoed, Christian, Espersen, R., Højgaard, C., Winther, Jakob R., Willemoës, Martin, Wedin, I., Nuopponen, M., Vilske, S., Aimonen, K., Weydahl, I. E. K., Alenius, H., Norppa, H., Wolff, H., Wallin, E. H. R. & Vogel, U., 2015, In: Chemical Research in Toxicology. 28, 8, p. 1627-1635 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  44. 2014
  45. Published

    In Silico prediction of mutant HIV-1 proteases cleaving a target sequence

    Jensen, Jan Halborg, Willemoës, Martin, Winther, Jakob R. & De Vico, L., 2014, In: P L o S One. 9, 5, 11 p., 5.

    Research output: Contribution to journal › Journal article › Research › peer-review

  46. Published

    Catalytic site interactions in yeast OMP synthase

    Hansen, M. R., Barr, E. W., Jensen, K. F., Willemoës, Martin, Grubmeyer, C. & Winther, Jakob R., 2014, In: Archives of Biochemistry and Biophysics. 542, p. 28-38 11 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  47. Published

    Non-invasive in-cell determination of free cytosolic [NAD+]/[NADH] ratios using hyperpolarized glucose show large variations in metabolic phenotypes

    Christensen, Caspar Elo, Karlsson, M., Winther, Jakob R., Jensen, P. R. & Lerche, M. H., 2014, In: The Journal of Biological Chemistry. 289, 4, p. 2344-2352 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  48. Published

    Quantification of thiols and disulfides

    Winther, Jakob R. & Thorpe, C., 2014, In: BBA General Subjects. 1840, 2, p. 838-846 9 p.

    Research output: Contribution to journal › Review › Research › peer-review

  49. Published

    Rapid TaqMan-based quantification of chlorophyll d-containing cyanobacteria in the genus Acaryochloris

    Behrendt, L., Nielsen, J. L., Sørensen, Søren Johannes, Larkum, A. W. D., Winther, Jakob R. & Kühl, Michael, 2014, In: Applied and Environmental Microbiology. 80, 10, p. 3244-3249 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  50. Published

    The pKa value and accessibility of cysteine residues are key determinants for protein substrate discrimination by glutaredoxin

    Jensen, K. S., Pedersen, J. T., Winther, Jakob R. & Teilum, Kaare, 2014, In: Biochemistry. 53, 15, p. 2533-2540 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  51. 2013
  52. Published

    Intracellular glutathione pools are heterogeneously concentrated

    Montero, D., Tachibana, C., Winther, Jakob R. & Appenzeller-Herzog, C., 2013, In: Redox Biology. 1, 1, p. 508-513 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  53. Published

    Quantifying changes in the cellular thiol-disulfide status during differentiation of B cells into antibody-secreting plasma cells

    Hansen, R. R. E., Otsu, M., Braakman, I. & Winther, Jakob R., 2013, In: International Journal of Cell Biology. 2013, 10 p., 898563.

    Research output: Contribution to journal › Journal article › Research › peer-review

  54. Published

    Redox control: a black hole for oxidized glutathione

    Winther, Jakob R. & Jakob, U., 2013, In: Nature Chemical Biology. 9, 2, p. 69-70 2 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  55. Published

    Saccharopepsin

    Winther, Jakob R., Webb, H. & Kay, J., 2013, Handbook of Proteolytic Enzymes. Rawlings, N. D. & Salvesen, G. (eds.). Third Edition ed. Academic Press, Vol. 1. p. 128-133 6 p.

    Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

  56. 2012
  57. Published

    A fluorescent probe which allows highly specific thiol labeling at low pH

    Nielsen, J. W., Jensen, K. S., Hansen, R. R. E., Gotfredsen, C. H. & Winther, Jakob R., 2012, In: Analytical Biochemistry. 421, 1, p. 115-120 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  58. Published

    Metal-ion dependent catalytic properties of Sulfolobus solfataricus class II α-mannosidase

    Nielsen, J. W., Poulsen, N. R., Johnsson, A. M. S., Winther, Jakob R., Stipp, S. L. S. & Willemoës, Martin, 2012, In: Biochemistry. 51, 40, p. 8039-8046 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  59. Published

    Polysaccharide effects on calcite growth: the influence of composition and branching

    Nielsen, J. W., Sand, Karina Krarup, Pedersen, C. S., Lakshtanov, L. Z., Winther, Jakob R., Willemoës, Martin & Stipp, S. L. S., 2012, In: Crystal Growth & Design. 12, 10, p. 4906-4910 5 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  60. Published

    The human selenoprotein VCP-interacting membrane protein (VIMP) is non-globular and harbors a reductase function in an intrinsically disordered region

    Christensen, L. C., Jensen, N. W., Lages Lino Vala, A., Kamarauskaite, J., Johansson, L. C., Winther, Jakob R., Hofmann, K., Teilum, Kaare & Ellgaard, Lars, 2012, In: Journal of Biological Chemistry. 287, 31, p. 26388-26399 12 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  61. 2011
  62. Published

    Millisecond dynamics in glutaredoxin during catalytic turnover is dependent on substrate binding and absent in the resting states

    Jensen, K. S., Winther, Jakob R. & Teilum, Kaare, 2011, In: Journal of the American Chemical Society. 133, 9, p. 3034-42 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  63. Published

    Trisulfides in Proteins

    Nielsen, R. W., Tachibana, C., Hansen, N. E. & Winther, Jakob R., 2011, In: Antioxidants & Redox Signaling. 15, 1, p. 67-75 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  64. 2010
  65. Published

    Kinetic and thermodynamic properties of two barley thioredoxin h isozymes, HvTrxh1 and HvTrxh2

    Maeda, K., Hägglund, Per Mårten, Björnberg, O., Winther, Jakob R. & Svensson, B., 2010, In: FEBS Letters. 584, 15, p. 3376-3380 4 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  66. Published

    Thiol-Disulfide Exchange between Glutaredoxin and Glutathione

    Iversen, R., Andersen, P. A., Jensen, K. S., Winther, Jakob R. & Sigurskjold, B. W., 2010, In: Biochemistry. 49, 4, p. 810-820 11 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  67. 2009
  68. Published

    An introduction to methods for analyzing thiols and disulfides: Reactions, reagents, and practical considerations

    Hansen, R. E. & Winther, Jakob R., 2009, In: Analytical Biochemistry. 394, 2, p. 147-58 11 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  69. Published

    Kinetic and Thermodynamic Aspects of Cellular Thiol-Disulfide Redox Regulation

    Jensen, K. S., Hansen, R. E. & Winther, Jakob R., 2009, In: Antioxidants & Redox Signaling. 11, 5, p. 1047-1058

    Research output: Contribution to journal › Journal article › Research › peer-review

  70. Published

    Quantifying the global cellular thiol-disulfide status

    Hansen, R. E., Roth, D. & Winther, Jakob R., 2009, In: Proceedings of the National Academy of Science of the United States of America. 106, 2, p. 422-427 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  71. 2008
  72. Published

    High-resolution imaging of redox signaling in live cells through an oxidation-sensitive yellow fluorescent protein

    Maulucci, G., Labate, V., Mele, M., Panieri, E., Arcovito, G., Galeotti, T., Østergaard, H., Winther, Jakob R., De Spirito, M. & Pani, G., 2008, In: Science Signaling. 1, 43, p. pl3 1-15 15 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  73. Published

    Mutations in the RAM network confer resistance to the thiol oxidant 4,4'-dipyridyl disulfide

    López-Mirabal, H. R., Winther, Jakob R., Thorsen, M. & Kielland-Brandt, M. C., 2008, In: Molecular Genetics and Genomics. 279, 6, p. 629-42 13 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  74. Published

    Oxidant resistance in a yeast mutant deficient in the Sit4 phosphatase

    López-Mirabal, H. R., Winther, Jakob R. & Kielland-Brandt, M. C., 2008, In: Current Genetics. 53, 5, p. 275-86 11 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  75. 2007
  76. Published

    Cytoplasmic glutathione redox status determines survival upon exposure to the thiol-oxidant 4,4'-dipyridyl disulfide.

    López-Mirabal, H. R., Thorsen, M., Kielland-Brandt, M. C., Toledano, M. B. & Winther, Jakob R., 2007, In: FEMS Yeast Research. 7, 3, p. 391-403 12 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  77. Published

    Erv2p: characterization of the redox behavior of a yeast sulfhydryl oxidase.

    Wang, W., Winther, Jakob R. & Thorpe, C., 2007, In: Biochemistry. 46, 11, p. 3246-54 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  78. Published

    Genetic interaction between the ero1-1 and leu2 mutations in Saccharomyces cerevisiae.

    López-Mirabal, H. R., Winther, Jakob R. & Kielland-Brandt, M. C., 2007, In: Bioscience, Biotechnology, and Biochemistry. 71, 12, p. 2934-42 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  79. Published

    Quantification of protein thiols and dithiols in the picomolar range using sodium borohydride and 4,4'-dithiodipyridine.

    Hansen, R. E., Østergaard, H., Nørgaard, P. & Winther, Jakob R., 2007, In: Analytical Biochemistry. 363, 1, p. 77-82 5 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  80. Published

    Redox characteristics of the eukaryotic cytosol.

    López-Mirabal, H. R. & Winther, Jakob R., 2007, In: BBA General Subjects.

    Research output: Contribution to journal › Journal article › Research › peer-review

  81. Published

    Structure of glutaredoxin Grx1p C30S mutant from yeast.

    Håkansson, K. O. & Winther, Jakob R., 2007, In: Acta Crystallographica. Section D: Biological Crystallography. 63, Pt 3, p. 288-94 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  82. Published

    The thiol oxidant dipyridyl disulfide can supply the PDI-Ero1p pathway with additional oxidative equivalents.

    López-Mirabal, H. R. & Winther, Jakob R., 2007, In: Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology. 92, 4, p. 463-72 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  83. 2006
  84. Published

    Crystallization of mutant forms of glutaredoxin Grx1p from yeast

    Håkansson, K. O., Østergaard, H. & Winther, Jakob R., 2006, In: Acta Crystallographica. Section F : Structural Biology and Crystallization Communications. 62, 9, p. 920-922

    Research output: Contribution to journal › Journal article › Research › peer-review

  85. Published

    Evidence that translation reinitiation leads to a partially functional Menkes protein containing two copper-binding sites

    Paulsen, M., Lund, C., Akram, Z., Winther, Jakob R., Horn, N. & Møller, L. B., 2006, In: American Journal of Human Genetics. 79, 2, p. 214-29 15 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  86. Published

    Measuring intracellular redox conditions using GFP-based sensors

    Björnberg, O., Ostergaard, H. & Winther, Jakob R., 2006, In: Antioxidants & Redox Signaling. 8, 3-4, p. 354-61 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  87. Published

    Mechanistic insight provided by glutaredoxin within a fusion to redox-sensitive yellow fluorescent protein

    Björnberg, O., Østergaard, H. & Winther, Jakob R., 2006, In: Biochemistry. 45, 7, p. 2362-71 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  88. 2005
  89. Published

    Increasing the reactivity of an artificial dithiol-bisulfide pair through modification of the electrostatic milieu

    Hansen, R. E., Østergaard, H. & Winther, Jakob R., 2005, In: Biochemistry.

    Research output: Contribution to journal › Journal article › Research › peer-review

  90. Published

    Increasing the reactivity of an artificial dithiol-disulfide pair through modification of the electrostatic milieu

    Hansen, R. E., Østergaard, H. & Winther, Jakob R., 2005, In: Biochemistry. 44, 15, p. 5899-906 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  91. 2004

  92. Fluorometric polyethyleneglycol-peptide hybrid substrates for quantitative assay of protein disulfide isomerase

    Christiansen, C., Hilaire, P. M. S. & Winther, Jakob R., 2004, In: Analytical Biochemistry. 333, 1, p. 148-155 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  93. Monitoring disulfide bond formation in the eukaryotic cytosol

    Østergaard, H., Tachibana, C. & Winther, Jakob R., 2004, In: Journal of Cell Biology. 166, 3, p. 337-345 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  94. Saccharopepsin

    Winther, Jakob R., Phylip, L. H. & Kay, J., 2004, Aspartic and Metallo Peptidases. Barrett, A. J., Rawlings, N. D. & Woessner, J. F. (eds.). 2. ed. Elsevier, p. 87-90 4 p. (Handbook of Proteolytic Enzymes, Vol. 1).

    Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

  95. The Contributions of Protein Disulfide Isomerase and Its Homologues to Oxidative Protein Folding in the Yeast Endoplasmic Reticulum

    Xiao, R., Wilkinson, B., Solovyov, A., Winther, Jakob R., Holmgren, A., Lundström-Ljung, J. & Gilbert, H. F., 2004, In: Journal of Biological Chemistry. 279, 48, p. 49780-49786 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  96. 2003

  97. Gene regulation in response to protein disulphide isomerase deficiency

    Nørgaard, P., Tachibana, C., Bruun, A. W. & Winther, Jakob R., 2003, In: Yeast. 20, 7, p. 645-652 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  98. 2001

  99. Barley lipid transfer protein, LTP1, contains a new type of lipid-like post-translational modification

    Lindorff-Larsen, K., Lerche, M. H., Poulsen, F. M., Roepstorff, P. & Winther, Jakob R., 2001, In: Journal of Biological Chemistry. 276, 36, p. 33547-53 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  100. Functional differences in yeast protein disulfide isomerases

    Nørgaard, P., Westphal, V., Tachibana, C., Alsøe, L., Holst, B. & Winther, Jakob R., 2001, In: Journal of Cell Biology. 152, 3, p. 553-562 10 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  101. Mutation of yeast Eug1p CXXS active sites to CXXC results in a dramatic increase in protein disulphide isomerase activity

    Nørgaard, P. & Winther, Jakob R., 2001, In: Biochemical Journal. 358, 1, p. 269-274 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  102. Production of a heterologous proteinase A by Saccharomyces kluyveri

    Møller, K., Tidemand, L. D., Winther, Jakob R., Olsson, L., Piskur, J. & Nielsen, J., 2001, In: Applied Microbiology and Biotechnology. 57, 1-2, p. 216-219 4 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  103. Shedding light on disulfide bond formation: engineering a redox switch in green fluorescent protein

    Østergaard, H., Henriksen, A., Hansen, F. G. & Winther, Jakob R., 2001, In: EMBO Journal. 20, 21, p. 5853-5862 10 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  104. Surprisingly high stability of barley lipid transfer protein, LTP1, towards denaturant, heat and proteases

    Lindorff-Larsen, Kresten & Winther, Jakob R., 2001, In: FEBS Letters. 488, 3, p. 145-148 4 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  105. The Potency and Specificity of the Interaction between the IA3 Inhibitor and Its Target Aspartic Proteinase from Saccharomyces cerevisiae

    Phylip, L. H., Lees, W. E., Brownsey, B. G., Bur, D., Dunn, B. M., Winther, Jakob R., Gustchina, A., Li, M., Copeland, T., Wlodawer, A. & Kay, J., 2001, In: Journal of Biological Chemistry. 276, 3, p. 2023-2030 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  106. 2000
  107. Published

    Purification, co-crystallization and preliminary X-ray analysis of the natural aspartic proteinase inhibitor I(A)3 complexed with saccharopepsin from Saccharomyces cerevisiae

    Badasso, M. O., Read, J. A., Dhanaraj, V., Cooper, J. B., Wood, S. P., Blundell, T. L., Dreyer, T. & Winther, Jakob R., 2000, In: Acta Crystallographica Section D: Biological Crystallography.

    Research output: Contribution to journal › Journal article › Research › peer-review

  108. The aspartic proteinase from Saccharomyces cerevisiae folds its own inhibitor into a helix

    Li, M., Phylip, L. H., Lees, W. E., Winther, Jakob R., Dunn, B. M., Wlodawer, A., Kay, J. & Gustchina, A., 2000, In: Nature Structural & Molecular Biology. 7, 2, p. 113-117 5 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  109. 1999

  110. Functional properties of the two redox-active sites in yeast protein disulphide isomerase in vitro and in vivo

    Westphal, V., Darby, N. J. & Winther, Jakob R., 1999, In: Journal of Molecular Biology. 286, 4, p. 1229-1239 11 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  111. Ligand recognition and domain structure of Vps10p, a vacuolar protein sorting receptor in Saccharomyces cerevisiae

    Jørgensen, M. U., Emr, S. D. & Winther, Jakob R., 1999, In: European Journal of Biochemistry. 260, 2, p. 461-469 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  112. Protein oxidation: prime suspect found 'not guilty'

    Bader, M., Winther, Jakob R. & Bardwell, J. C. A., 1999, In: Nature Cell Biology. 1, 3, p. E57-E58

    Research output: Contribution to journal › Comment/debate › Communication

  113. 1998

  114. A High-Affinity Inhibitor of Yeast Carboxypeptidase Y Is Encoded by TFS1 and Shows Homology to a Family of Lipid Binding Proteins

    Bruun, A. W., Svendsen, I., Sørensen, S. O., Kielland-Brandt, M. C. & Winther, Jakob R., 1998, In: Biochemistry. 37, 10, p. 3351-3357 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  115. Published

    Kinetic analysis of the mechanism and specificity of protein-disulfide isomerase using fluorescence-quenched peptides

    Westphal, V., Spetzler, J. C., Meldal, Morten Peter, Christensen, U. & Winther, Jakob R., 1998, In: Journal of Biological Chemistry. 273, 39, p. 24992-9 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  116. Preparation of fluorescence quenched libraries containing interchain disulphide bonds for studies of protein disulphide isomerases

    Spetzler, J. C., Westphal, V., Winther, Jakob R. & Meldal, M., 1998, In: Journal of Peptide Science. 4, 2, p. 128-137 10 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  117. 1997

  118. Active site mutations in yeast protein disulfide isomerase cause dithiothreitol sensitivity and a reduced rate of protein folding in the endoplasmic reticulum

    Holst, B., Tachibana, C. & Winther, Jakob R., 1997, In: Journal of Cell Biology. 138, 6, p. 1229-1238 10 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  119. Published

    Mechanism and ion-dependence of in vitro autoactivation of yeast proteinase A: possible implications for compartmentalized activation in vivo

    Van Den Hazel, H., Wolff, A. M., Kielland-Brandt, M. & Winther, Jakob R., 1997, In: Biochemical Journal. 326 ( Pt 2), p. 339-44 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  120. 1996
  121. Published

    Competition between folding and glycosylation in the endoplasmic reticulum

    Holst, B., Bruun, A. W., Kielland-Brandt, M. & Winther, Jakob R., 1996, In: E M B O Journal. 15, 14, p. 3538-46 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  122. Published

    Exchange of regions of the carboxypeptidase Y propeptide. Sequence specificity and function in folding in vivo

    Ramos, C. & Winther, Jakob R., 1996, In: European Journal of Biochemistry. 242, 1, p. 29-35 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  123. Published

    Multiple pathways for vacuolar sorting of yeast proteinase A

    Westphal, V., Marcusson, E. G., Winther, Jakob R., Emr, S. D. & van den Hazel, H. B., 1996, In: Journal of Biological Chemistry. 271, 20, p. 11865-70 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  124. Published

    Mutational analysis of the vacuolar sorting signal of procarboxypeptidase Y in yeast shows a low requirement for sequence conservation

    van Voorst, F., Kielland-Brandt, M. & Winther, Jakob R., 1996, In: Journal of Biological Chemistry. 271, 2, p. 841-6 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  125. Published

    Review: biosynthesis and function of yeast vacuolar proteases

    Van Den Hazel, H. B., Kielland-Brandt, M. & Winther, Jakob R., 1996, In: Yeast (Chichester, England). 12, 1, p. 1-16 16 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  126. 1995
  127. Published

    Random substitution of large parts of the propeptide of yeast proteinase A

    van den Hazel, H. B., Kielland-Brandt, M. & Winther, Jakob R., 1995, In: Journal of Biological Chemistry. 270, 15, p. 8602-9 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  128. Published

    Why is DsbA such an oxidizing disulfide catalyst?

    Grauschopf, U., Winther, Jakob R., Korber, P., Zander, T., Dallinger, P. & Bardwell, J. C., 1995, In: Cell. 83, 6, p. 947-55 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  129. 1994
  130. Published

    Active-site residues of procarboxypeptidase Y are accessible to chemical modification

    Sørensen, S. O. & Winther, Jakob R., 1994, In: BBA General Subjects. 1205, 2, p. 289-93 5 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  131. Published

    Refolding of a carboxypeptidase Y folding intermediate in vitro by low-affinity binding of the proregion

    Winther, Jakob R., Sørensen, P. & Kielland-Brandt, M., 1994, In: Journal of Biological Chemistry. 269, 35, p. 22007-13 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  132. Published

    Requirement of the propeptide for in vivo formation of active yeast carboxypeptidase Y

    Ramos, C., Winther, Jakob R. & Kielland-Brandt, M., 1994, In: Journal of Biological Chemistry. 269, 9, p. 7006-12 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  133. Published

    pH-dependent processing of yeast procarboxypeptidase Y by proteinase A in vivo and in vitro

    Sørensen, S. O., van den Hazel, H. B., Kielland-Brandt, M. & Winther, Jakob R., 1994, In: European Journal of Biochemistry. 220, 1, p. 19-27 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  134. 1993
  135. Published

    The pro region required for folding of carboxypeptidase Y is a partially folded domain with little regular structural core

    Sørensen, P., Winther, Jakob R., Kaarsholm, N. C. & Poulsen, F. M., 1993, In: Biochemistry. 32, 45, p. 12160-6 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  136. Published

    The propeptide is required for in vivo formation of stable active yeast proteinase A and can function even when not covalently linked to the mature region

    van den Hazel, H. B., Kielland-Brandt, M. & Winther, Jakob R., 1993, In: Journal of Biological Chemistry. 268, 24, p. 18002-7 6 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  137. 1992
  138. Published

    Autoactivation of proteinase A initiates activation of yeast vacuolar zymogens

    van den Hazel, H. B., Kielland-Brandt, M. & Winther, Jakob R., 1992, In: European Journal of Biochemistry. 207, 1, p. 277-83 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  139. 1991
  140. Published

    Propeptide of carboxypeptidase Y provides a chaperone-like function as well as inhibition of the enzymatic activity

    Winther, Jakob R. & Sørensen, P., 1991, In: Proceedings of the National Academy of Sciences of the United States of America. 88, 20, p. 9330-4 5 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  141. Published

    Yeast carboxypeptidase Y requires glycosylation for efficient intracellular transport, but not for vacuolar sorting, in vivo stability, or activity

    Winther, Jakob R., Stevens, T. H. & Kielland-Brandt, M., 1991, In: European Journal of Biochemistry. 197, 3, p. 681-9 9 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  142. 1990
  143. Published

    Yeast carboxypeptidase Y vacuolar targeting signal is defined by four propeptide amino acids

    Valls, L. A., Winther, Jakob R. & Stevens, T. H., 1990, In: Journal of Cell Biology. 111, 2, p. 361-8 8 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  144. 1987
  145. Published

    THE FREE SULFHYDRYL-GROUP (CYS341) OF CARBOXYPEPTIDASE-Y - FUNCTIONAL-EFFECTS OF MUTATIONAL SUBSTITUTIONS

    WINTHER, J., BREDDAM, K. & Winther, Jakob R., 1987, In: Carlsberg Research Communications. 52, 4, p. 263-273

    Research output: Contribution to journal › Journal article › Research › peer-review

  146. 1986
  147. Published

    Mutational replacement of methionine by arginine in the S′1 substrate binding site of yeast carboxypeptidase

    Bech, L. M., Nielsen, J., Winther, Jakob R., Kielland-Brandt, M. C. & Breddam, K., 1986, In: Carlsberg Research Communications. 51, 6, p. 459-465 7 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

  148. 1985

  149. Increased hydrophobicity of the S′1 binding site in carboxypeptidase Y obtained by site-directed mutagenesis

    Winther, Jakob R., Kielland-Brandt, M. C. & Breddam, K., 1985, In: Carlsberg Research Communications. 50, 5, p. 273-284 12 p.

    Research output: Contribution to journal › Journal article › Research › peer-review

ID: 5143