7-Deaza purine bases offer a higher ion stability in the analysis of dna by matrix-assisted laser desorption/ionization mass spectrometry
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7-Deaza purine bases offer a higher ion stability in the analysis of dna by matrix-assisted laser desorption/ionization mass spectrometry. / Kirpekar, F.; Nordhoff, E.; Kristiansen, Karsten; Roepstorff, P.; Hahner, S.; Hillenkamp, F.
I: Rapid Communications in Mass Spectrometry, Bind 9, Nr. 6, 1995, s. 525 - 531.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - 7-Deaza purine bases offer a higher ion stability in the analysis of dna by matrix-assisted laser desorption/ionization mass spectrometry
AU - Kirpekar, F.
AU - Nordhoff, E.
AU - Kristiansen, Karsten
AU - Roepstorff, P.
AU - Hahner, S.
AU - Hillenkamp, F.
PY - 1995
Y1 - 1995
N2 - Oligodeoxynucleotides which contain 7-deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified Oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI-MS with positive ion detection mode are presented. Additionally, the potential use of 7-deaza purine nucleotides in the MALDI-MS analysis of DNA sequencing reactions is discussed.
AB - Oligodeoxynucleotides which contain 7-deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified Oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI-MS with positive ion detection mode are presented. Additionally, the potential use of 7-deaza purine nucleotides in the MALDI-MS analysis of DNA sequencing reactions is discussed.
U2 - 10.1002/rcm.1290090611
DO - 10.1002/rcm.1290090611
M3 - Journal article
VL - 9
SP - 525
EP - 531
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
SN - 0951-4198
IS - 6
ER -
ID: 11231924