Active-site residues of procarboxypeptidase Y are accessible to chemical modification
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Active-site residues of procarboxypeptidase Y are accessible to chemical modification. / Sørensen, S O; Winther, Jakob R.
I: BBA General Subjects, Bind 1205, Nr. 2, 1994, s. 289-93.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Active-site residues of procarboxypeptidase Y are accessible to chemical modification
AU - Sørensen, S O
AU - Winther, Jakob R.
PY - 1994
Y1 - 1994
N2 - The accessibility of the active-site cleft of procarboxypeptidase Y from Saccharomyces cerevisiae has been studied by chemical modifications of two specific amino-acid residues. Previous studies have shown that these residues, Cys-341 and Met-398 in the mature enzyme, are located in the S1 and S'1 substrate binding sites, respectively, of carboxypeptidase Y. We have found that these residues also in proCPY are accessible to modification with fairly bulky reagents and in the case of Met-398 the rate of modification is even faster than in carboxypeptidase Y. While the catalytic serine in the mature enzyme reacts with diisopropylfluorophosphate, this is not the case for procarboxypeptidase Y.
AB - The accessibility of the active-site cleft of procarboxypeptidase Y from Saccharomyces cerevisiae has been studied by chemical modifications of two specific amino-acid residues. Previous studies have shown that these residues, Cys-341 and Met-398 in the mature enzyme, are located in the S1 and S'1 substrate binding sites, respectively, of carboxypeptidase Y. We have found that these residues also in proCPY are accessible to modification with fairly bulky reagents and in the case of Met-398 the rate of modification is even faster than in carboxypeptidase Y. While the catalytic serine in the mature enzyme reacts with diisopropylfluorophosphate, this is not the case for procarboxypeptidase Y.
KW - Acetophenones
KW - Binding Sites
KW - Carboxypeptidases
KW - Cathepsin A
KW - Enzyme Precursors
KW - Enzyme Stability
KW - Isoflurophate
KW - Mercuric Chloride
KW - Phenylmercury Compounds
KW - Protein Conformation
KW - Saccharomyces cerevisiae
KW - Saccharomyces cerevisiae Proteins
M3 - Journal article
C2 - 8155711
VL - 1205
SP - 289
EP - 293
JO - B B A - General Subjects
JF - B B A - General Subjects
SN - 0304-4165
IS - 2
ER -
ID: 43974409