Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II

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Standard

Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II. / McGuire, Kirsten Arnvig; McGuire, J.N.; Wettstein-Knowles, Penny von.

I: Biochemical Society Transactions, Bind 28 part 6, 2000, s. 607-610.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

McGuire, KA, McGuire, JN & Wettstein-Knowles, PV 2000, 'Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II', Biochemical Society Transactions, bind 28 part 6, s. 607-610. <http://www.biochemsoctrans.org/bst/028/bst0280607.htm>

APA

McGuire, K. A., McGuire, J. N., & Wettstein-Knowles, P. V. (2000). Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II. Biochemical Society Transactions, 28 part 6, 607-610. http://www.biochemsoctrans.org/bst/028/bst0280607.htm

Vancouver

McGuire KA, McGuire JN, Wettstein-Knowles PV. Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II. Biochemical Society Transactions. 2000;28 part 6:607-610.

Author

McGuire, Kirsten Arnvig ; McGuire, J.N. ; Wettstein-Knowles, Penny von. / Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II. I: Biochemical Society Transactions. 2000 ; Bind 28 part 6. s. 607-610.

Bibtex

@article{3862c58074c811dbbee902004c4f4f50,
title = "Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II",
abstract = "Escherichia coli b-ketoacyl synthases (KAS) I and II carry out the elongation steps in fatty acid synthesis. Analyses using the cross-linker BS3 [bis(sulphosuccinimidyl) suberate] and surface-enhanced laser desorption/ionization–time-of-flight MS disclosed only monomeric and dimeric forms of KAS II, whereas KAS I also forms higher multimers. The binding affinities for KAS I and KAS II to C14-acyl carrier protein (ACP) as well as for C14-ACP to KAS I and KAS II were determined. KAS I is sensitive to the ACP released during the transfer reaction, with 50% inhibition at 0.17 µM ACP close to the physiological concentration of ACP (0.13 µM). KAS I and II also differ in carrying out the decarboxylation step of the elongation reaction.",
author = "McGuire, {Kirsten Arnvig} and J.N. McGuire and Wettstein-Knowles, {Penny von}",
year = "2000",
language = "English",
volume = "28 part 6",
pages = "607--610",
journal = "Biochemical Society Transactions",
issn = "0300-5127",
publisher = "Portland Press Ltd.",

}

RIS

TY - JOUR

T1 - Acyl carrier protein (ACP) inhibition and other differences between b-ketoacyl synthase (KAS) I and II

AU - McGuire, Kirsten Arnvig

AU - McGuire, J.N.

AU - Wettstein-Knowles, Penny von

PY - 2000

Y1 - 2000

N2 - Escherichia coli b-ketoacyl synthases (KAS) I and II carry out the elongation steps in fatty acid synthesis. Analyses using the cross-linker BS3 [bis(sulphosuccinimidyl) suberate] and surface-enhanced laser desorption/ionization–time-of-flight MS disclosed only monomeric and dimeric forms of KAS II, whereas KAS I also forms higher multimers. The binding affinities for KAS I and KAS II to C14-acyl carrier protein (ACP) as well as for C14-ACP to KAS I and KAS II were determined. KAS I is sensitive to the ACP released during the transfer reaction, with 50% inhibition at 0.17 µM ACP close to the physiological concentration of ACP (0.13 µM). KAS I and II also differ in carrying out the decarboxylation step of the elongation reaction.

AB - Escherichia coli b-ketoacyl synthases (KAS) I and II carry out the elongation steps in fatty acid synthesis. Analyses using the cross-linker BS3 [bis(sulphosuccinimidyl) suberate] and surface-enhanced laser desorption/ionization–time-of-flight MS disclosed only monomeric and dimeric forms of KAS II, whereas KAS I also forms higher multimers. The binding affinities for KAS I and KAS II to C14-acyl carrier protein (ACP) as well as for C14-ACP to KAS I and KAS II were determined. KAS I is sensitive to the ACP released during the transfer reaction, with 50% inhibition at 0.17 µM ACP close to the physiological concentration of ACP (0.13 µM). KAS I and II also differ in carrying out the decarboxylation step of the elongation reaction.

M3 - Journal article

VL - 28 part 6

SP - 607

EP - 610

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

ER -

ID: 180327