Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning
Determining the stability of plasmids in bacterial populations is traditionally performed by isolating a large number of clones followed by screening for the presence of plasmids by replica transfer to plasmid-selective agar plates. This is often a laborious task, especially when the intrinsic stability of the plasmid is high. The method presented here relies on a phenotypic (green fluorescence protein) marker, which is switched on if the host bacteria loses the residing plasmid. The incorporation of flow cytometry for single-cell detection and discrimination between plasmid-free and plasmid-harboring cells in a bacterial population facilitates a very high throughput of cells and thus provides excellent sensitivity and statistics toward detecting even very low levels of plasmid instability.
Originalsprog | Engelsk |
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Titel | Horizontal Gene Transfer |
Redaktører | Maria Boekels Gogarten, Johann Peter Gogarten, Lorraine C. Olendzenski |
Vol/bind | 532 |
Forlag | Humana Press |
Publikationsdato | 2009 |
Sider | 257-268 |
ISBN (Trykt) | 978-1-60327-852-2 |
ISBN (Elektronisk) | 978-1-60327-853-9 |
DOI | |
Status | Udgivet - 2009 |
Navn | Methods in Molecular Biology |
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ISSN | 1064-3745 |
ID: 11663620